MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 230)


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-71

Accession Score Description
1 RL22_PSEA7 96 50S ribosomal protein L22 OS=Pseudomonas aeruginosa (strain PA7) GN=rplV PE=3 SV=1
Score Mass Matches Sequences emPAI
71.1 RL22_PSEA7 96 11961 5 (3) 4 (2) 0.73
50S ribosomal protein L22 OS=Pseudomonas aeruginosa (strain PA7) GN=rplV PE=3 SV=1
15 samesets of RL22_PSEA7
RL22_PSEA8 96 11961 5 (3) 4 (2) 0.73
50S ribosomal protein L22 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplV PE=3 SV=1
RL22_PSEAB 96 11961 5 (3) 4 (2) 0.73
50S ribosomal protein L22 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplV PE=3 SV=1
RL22_PSEAE 96 11961 5 (3) 4 (2) 0.73
50S ribosomal protein L22 OS=Pseudomonas aeruginosa GN=rplV PE=3 SV=1
RL22_PSEE4 96 11961 5 (3) 4 (2) 0.73
50S ribosomal protein L22 OS=Pseudomonas entomophila (strain L48) GN=rplV PE=3 SV=1
RL22_PSEF5 96 12092 5 (3) 4 (2) 0.72
50S ribosomal protein L22 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplV PE=3 SV=1
RL22_PSEFS 96 11961 5 (3) 4 (2) 0.73
50S ribosomal protein L22 OS=Pseudomonas fluorescens (strain SBW25) GN=rplV PE=3 SV=1
RL22_PSEMY 96 11961 5 (3) 4 (2) 0.73
50S ribosomal protein L22 OS=Pseudomonas mendocina (strain ymp) GN=rplV PE=3 SV=1
RL22_PSEP1 96 11961 5 (3) 4 (2) 0.73
50S ribosomal protein L22 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplV PE=3 SV=1
RL22_PSEPF 96 11961 5 (3) 4 (2) 0.73
50S ribosomal protein L22 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplV PE=3 SV=1
RL22_PSEPG 96 11961 5 (3) 4 (2) 0.73
50S ribosomal protein L22 OS=Pseudomonas putida (strain GB-1) GN=rplV PE=3 SV=1
RL22_PSEPK 96 11961 5 (3) 4 (2) 0.73
50S ribosomal protein L22 OS=Pseudomonas putida (strain KT2440) GN=rplV PE=3 SV=1
RL22_PSEPW 96 11961 5 (3) 4 (2) 0.73
50S ribosomal protein L22 OS=Pseudomonas putida (strain W619) GN=rplV PE=3 SV=1
RL22_PSE14 96 11943 4 (3) 3 (2) 0.73
50S ribosomal protein L22 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplV PE=3 SV=1
RL22_PSESM 96 11943 4 (3) 3 (2) 0.73
50S ribosomal protein L22 OS=Pseudomonas syringae pv. tomato GN=rplV PE=3 SV=1
RL22_PSEU2 96 11943 4 (3) 3 (2) 0.73
50S ribosomal protein L22 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplV PE=3 SV=1

-5 peptide matches (5 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
388   331.6856 661.3566 661.3469 14.8 1 17 2.4 +5Score > 42 indicates identity
Score > 33 indicates homology
U K.AAEIMK.K
703   407.7442 813.4738 813.4708 3.70 0 30 0.31 +1Score > 41 indicates identity
Score > 37 indicates homology
R.LVADQIR.G
1368   554.2952 1106.5758 1106.5720 3.44 1 60 0.00011 +1Score > 41 indicates identity
Score > 33 indicates homology
U K.VSTVFVNEGR.S
1863   674.8824 1347.7502 1347.7398 7.76 1 62 2.8e-005 +1Score > 38 indicates identity
Score > 29 indicates homology
U K.VGEALNLLAFSSK.K
1867   675.3793 1348.7440 1348.7238 15.0 1 34 0.016 +1Score > 38 indicates identity
Score > 28 indicates homology
U K.VGEALNLLAFSSK.K + Deamidated (NQ)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
RL22_PSEU5 60 11947 71.1
50S ribosomal protein L22 OS=Pseudomonas stutzeri (strain A1501) GN=rplV PE=3 SV=1

+72

Accession Score Description
1 ODO1_COXBU 96 2-oxoglutarate dehydrogenase E1 component OS=Coxiella burnetii GN=sucA PE=3 SV=3

+73

Accession Score Description
1 GLNA_AZOVI 95 Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1

+74

Accession Score Description
1 KPRS_PSEPK 93 Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1

+75

Accession Score Description
1 NUOI_PSEPK 87 NADH-quinone oxidoreductase subunit I OS=Pseudomonas putida (strain KT2440) GN=nuoI PE=3 SV=1

+76

Accession Score Description
1 AROB_PSEPK 85 3-dehydroquinate synthase OS=Pseudomonas putida (strain KT2440) GN=aroB PE=3 SV=1

+77

Accession Score Description
1 RS18_PSEE4 84 30S ribosomal protein S18 OS=Pseudomonas entomophila (strain L48) GN=rpsR PE=3 SV=1

+78

Accession Score Description
1 ARCA_PSEPK 82 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+79

Accession Score Description
1 ATPE_PSEPG 82 ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1

+80

Accession Score Description
1 PUR5_PSEP1 80 Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=purM PE=3 SV=1
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