MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 230)


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+61

Accession Score Description
1 DHSB_ECOLI 100 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

+62

Accession Score Description
1 BETI_PSEF5 99 HTH-type transcriptional regulator betI OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=betI PE=3 SV=1

+63

Accession Score Description
1 ILVC_PSEPG 99 Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1

-64

Accession Score Description
1 RL6_PSEP1 99 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1
Score Mass Matches Sequences emPAI
64.1 RL6_PSEP1 99 19131 6 (3) 6 (3) 0.42
50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1
6 samesets of RL6_PSEP1
RL6_PSEPG 99 19131 6 (3) 6 (3) 0.42
50S ribosomal protein L6 OS=Pseudomonas putida (strain GB-1) GN=rplF PE=3 SV=1
RL6_PSEPK 99 19131 6 (3) 6 (3) 0.42
50S ribosomal protein L6 OS=Pseudomonas putida (strain KT2440) GN=rplF PE=3 SV=1
RL6_PSEF5 99 19170 5 (3) 5 (3) 0.41
50S ribosomal protein L6 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplF PE=3 SV=1
RL6_PSEFS 99 19185 5 (3) 5 (3) 0.41
50S ribosomal protein L6 OS=Pseudomonas fluorescens (strain SBW25) GN=rplF PE=3 SV=1
RL6_PSEPF 99 19184 5 (3) 5 (3) 0.41
50S ribosomal protein L6 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplF PE=3 SV=1
RL6_PSEPW 99 19115 5 (3) 5 (3) 0.42
50S ribosomal protein L6 OS=Pseudomonas putida (strain W619) GN=rplF PE=3 SV=1

+6 peptide matches (6 non-duplicate, 0 duplicate)

4 subsets and intersections (21 subset proteins in total)

Score Mass Subset of
RL6_PSE14 86 19506 64.1
50S ribosomal protein L6 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplF PE=3 SV=1
8 samesets of RL6_PSE14
RL6_PSESM 86 19266
50S ribosomal protein L6 OS=Pseudomonas syringae pv. tomato GN=rplF PE=3 SV=1
RL6_PSEU2 86 19236
50S ribosomal protein L6 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplF PE=3 SV=1
RL6_PSEA7 86 19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa (strain PA7) GN=rplF PE=3 SV=1
RL6_PSEA8 86 19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplF PE=3 SV=1
RL6_PSEAB 86 19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplF PE=3 SV=1
RL6_PSEAE 86 19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa GN=rplF PE=3 SV=1
RL6_PSEU5 86 19167
50S ribosomal protein L6 OS=Pseudomonas stutzeri (strain A1501) GN=rplF PE=3 SV=1
RL6_PSEMY 86 19130
50S ribosomal protein L6 OS=Pseudomonas mendocina (strain ymp) GN=rplF PE=3 SV=1
RL6_CHRVO 69 18841 64.1
50S ribosomal protein L6 OS=Chromobacterium violaceum GN=rplF PE=3 SV=1
3 samesets of RL6_CHRVO
RL6_SHELP 69 18777
50S ribosomal protein L6 OS=Shewanella loihica (strain ATCC BAA-1088 / PV-4) GN=rplF PE=3 SV=1
RL6_SHESH 69 18751
50S ribosomal protein L6 OS=Shewanella sediminis (strain HAW-EB3) GN=rplF PE=3 SV=1
RL6_SHEWM 69 18852
50S ribosomal protein L6 OS=Shewanella woodyi (strain ATCC 51908 / MS32) GN=rplF PE=3 SV=1
RL6_PSEE4 68 19015 64.1
50S ribosomal protein L6 OS=Pseudomonas entomophila (strain L48) GN=rplF PE=3 SV=1
RL6_ACIB3 52 19085 64.1
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain AB307-0294) GN=rplF PE=3 SV=1
6 samesets of RL6_ACIB3
RL6_ACIB5 52 19085
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain AB0057) GN=rplF PE=3 SV=1
RL6_ACIBS 52 19113
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain SDF) GN=rplF PE=3 SV=1
RL6_ACIBT 52 19085
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) GN=rplF PE=3 SV=2
RL6_ACIBY 52 19085
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain AYE) GN=rplF PE=3 SV=1
RL6_AERHH 52 18568
50S ribosomal protein L6 OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) GN=rplF PE=3 SV=1
RL6_AERS4 52 18556
50S ribosomal protein L6 OS=Aeromonas salmonicida (strain A449) GN=rplF PE=3 SV=1

+65

Accession Score Description
1 RS7_PSEE4 99 30S ribosomal protein S7 OS=Pseudomonas entomophila (strain L48) GN=rpsG PE=3 SV=1

+66

Accession Score Description
1 OPRI_PSEAE 99 Major outer membrane lipoprotein OS=Pseudomonas aeruginosa GN=oprI PE=3 SV=1

+67

Accession Score Description
1 RL25_PSEPK 98 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2

+68

Accession Score Description
1 FTSA_PSEAE 98 Cell division protein ftsA OS=Pseudomonas aeruginosa GN=ftsA PE=3 SV=2

+69

Accession Score Description
1 RL10_PSEPG 97 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+70

Accession Score Description
1 PCAB_PSEPU 97 3-carboxy-cis,cis-muconate cycloisomerase (Fragment) OS=Pseudomonas putida GN=pcaB PE=1 SV=3
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