MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 230)

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+51

Accession Score Description
1 RL14_PSE14 112 50S ribosomal protein L14 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplN PE=3 SV=1

+52

Accession Score Description
1 RL21_PSEE4 111 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1

+53

Accession Score Description
1 STHA_PSEPG 110 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1

+54

Accession Score Description
1 CARB_PSEPK 108 Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2

+55

Accession Score Description
1 SUCD_PSEAE 105 Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2

+56

Accession Score Description
1 GUAA_PSEPG 103 GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas putida (strain GB-1) GN=guaA PE=3 SV=1

+57

Accession Score Description
1 DNAK_PSEPK 102 Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1

+58

Accession Score Description
1 SYA_PSEPG 102 Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2

-59

Accession Score Description
1 EFTS_PSEPK 102 Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1
Score Mass Matches Sequences emPAI
59.1 EFTS_PSEPK 102 30527 3 (3) 3 (3) 0.24
Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1
1 sameset of EFTS_PSEPK
EFTS_PSEP1 102 30513 3 (3) 3 (3) 0.24
Elongation factor Ts OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=tsf PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
674   399.7778 797.5410 797.5375 4.48 0 47 0.0013 +1Score > 30 indicates identity U K.IGAVVVLK.G
2085   486.5859 1456.7359 1456.7310 3.31 1 41 0.0032 +1Score > 40 indicates identity
Score > 29 indicates homology		 U R.VEGDVVGAYLHGNK.I
4111   910.1217 2727.3433 2727.3127 11.2 1 73 2.3e-006 +1Score > 37 indicates identity
Score > 29 indicates homology		 U K.TDGTSAVLLEVNSQTDFLALQDDFK.N + Deamidated (NQ)

1 subset or intersection (3 subset proteins in total)

Score Mass Subset of
EFTS_PSEE4 58 30581 59.1
Elongation factor Ts OS=Pseudomonas entomophila (strain L48) GN=tsf PE=3 SV=1
2 samesets of EFTS_PSEE4
EFTS_PSEPG 58 30483
Elongation factor Ts OS=Pseudomonas putida (strain GB-1) GN=tsf PE=3 SV=1
EFTS_PSEPW 58 30452
Elongation factor Ts OS=Pseudomonas putida (strain W619) GN=tsf PE=3 SV=1

+60

Accession Score Description
1 TIG_PSEPK 100 Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2
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