MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 sp |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 22:50:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
51| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL14_PSE14 | 112 | 50S ribosomal protein L14 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplN PE=3 SV=1 |
52| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL21_PSEE4 | 111 | 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1 |
53| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | STHA_PSEPG | 110 | Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1 |
54| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CARB_PSEPK | 108 | Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2 |
55| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCD_PSEAE | 105 | Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2 |
56| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GUAA_PSEPG | 103 | GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas putida (strain GB-1) GN=guaA PE=3 SV=1 |
57| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_PSEPK | 102 | Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1 |
58| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYA_PSEPG | 102 | Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 58.1 | SYA_PSEPG |
102 | 95050 | 6 (4) | 5 (4) | 0.10 | |
| Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2 | |||||||
| 2 samesets of SYA_PSEPG | |||||||
| SYA_PSEPK |
102 | 95109 | 6 (4) | 5 (4) | 0.10 | ||
| Alanyl-tRNA synthetase OS=Pseudomonas putida (strain KT2440) GN=alaS PE=3 SV=1 | |||||||
| SYA_PSEP1 |
102 | 95108 | 6 (4) | 5 (4) | 0.10 | ||
| Alanyl-tRNA synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=alaS PE=3 SV=2 | |||||||
6 peptide matches (6 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 510 |
360.7375 | 719.4604 | 719.4581 | 3.22 | 0 | 29 | 0.056 | 1Score > 29 indicates identity |
U | K.IIALYK.D | |
| 1317 |
542.3464 | 1082.6782 | 1082.6699 | 7.69 | 0 | 32 | 0.054 | 1Score > 32 indicates identity |
U | K.ALLALVDQLK.N | |
| 1523 |
585.8439 | 1169.6732 | 1169.6656 | 6.57 | 1 | 29 | 0.097 | 1Score > 38 indicates identity Score > 32 indicates homology |
U | R.ILEQDLAQLK.G | |
| 2373 |
794.8977 | 1587.7808 | 1587.7740 | 4.31 | 1 | 88 | 7.7e-008 | 1Score > 41 indicates identity Score > 29 indicates homology |
U | K.AASAAGDDLSNAAVEVK.G | |
| 4220 |
713.8875 | 2851.5209 | 2851.4868 | 12.0 | 1 | 33 | 0.0013 | 1Score > 34 indicates identity Score > 17 indicates homology |
U | R.VSAVLQHVHSNYEIDLFQNLLAAAAK.A + Deamidated (NQ) | |
| 4221 |
951.5157 | 2851.5253 | 2851.4868 | 13.5 | 1 | 17 | 1 | 1Score > 34 indicates identity Score > 30 indicates homology |
U | R.VSAVLQHVHSNYEIDLFQNLLAAAAK.A + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| SYA_PSEE4 |
90 | 95309 | 58.1 | ||
| Alanyl-tRNA synthetase OS=Pseudomonas entomophila (strain L48) GN=alaS PE=3 SV=1 | |||||
| SYA_PSE14 |
32 | 94784 | 58.1 | ||
| Alanyl-tRNA synthetase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=alaS PE=3 SV=1 | |||||
| 10 samesets of SYA_PSE14 | |||||
| SYA_PSEPW |
32 | 94991 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas putida (strain W619) GN=alaS PE=3 SV=2 | |||||
| SYA_PSEMY |
32 | 94797 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas mendocina (strain ymp) GN=alaS PE=3 SV=2 | |||||
| SYA_PSEAB |
32 | 95112 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=alaS PE=3 SV=1 | |||||
| SYA_PSEAE |
32 | 95099 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas aeruginosa GN=alaS PE=3 SV=1 | |||||
| SYA_PSEPF |
32 | 94840 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas fluorescens (strain Pf0-1) GN=alaS PE=3 SV=1 | |||||
| SYA_PSESM |
32 | 94865 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas syringae pv. tomato GN=alaS PE=3 SV=1 | |||||
| SYA_PSEU2 |
32 | 94751 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=alaS PE=3 SV=2 | |||||
| SYA_PSEF5 |
32 | 95022 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=alaS PE=3 SV=1 | |||||
| SYA_PSEU5 |
32 | 95029 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas stutzeri (strain A1501) GN=alaS PE=3 SV=2 | |||||
| SYA_PSEA7 |
32 | 94997 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas aeruginosa (strain PA7) GN=alaS PE=3 SV=1 | |||||
| BIOB_CARHZ |
29 | 36628 | 58.1 | ||
| Biotin synthase OS=Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) GN=bioB PE=3 SV=1 | |||||
| 41 samesets of BIOB_CARHZ |
|||||
59| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTS_PSEPK | 102 | Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1 |
60| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TIG_PSEPK | 100 | Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2 |
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