MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 sp |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 22:50:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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51| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL14_PSE14 | 112 | 50S ribosomal protein L14 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplN PE=3 SV=1 |
52| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL21_PSEE4 | 111 | 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1 |
53| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | STHA_PSEPG | 110 | Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1 |
54| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CARB_PSEPK | 108 | Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2 |
55| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCD_PSEAE | 105 | Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 55.1 | SUCD_PSEAE |
105 | 30646 | 5 (3) | 5 (3) | 0.24 | |
| Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2 | |||||||
5 peptide matches (5 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 347 |
315.7018 | 629.3890 | 629.3861 | 4.74 | 0 | 22 | 3.3 | 3Score > 41 indicates identity Score > 39 indicates homology |
K.VGIVSR.S | ||
| 1191 |
510.2806 | 1018.5466 | 1018.5447 | 1.87 | 0 | 11 | 1.3 | 3Score > 41 indicates identity Score > 25 indicates homology |
U | K.FAALQDAGVK.T | |
| 1282 |
534.7840 | 1067.5534 | 1067.5499 | 3.34 | 1 | 73 | 7.7e-006 | 1Score > 40 indicates identity Score > 34 indicates homology |
U | R.SGTLTYEAVK.Q | |
| 1628 |
610.8345 | 1219.6544 | 1219.6448 | 7.88 | 1 | 58 | 0.00019 | 1Score > 40 indicates identity Score > 33 indicates homology |
U | K.DSILEAAFGGIK.L | |
| 2272 |
514.2925 | 1539.8557 | 1539.8410 | 9.56 | 0 | 33 | 0.0033 | 1Score > 37 indicates identity Score > 20 indicates homology |
U | K.GGTTHLGLPVFNTVK.E |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| SUCD_ECO57 |
73 | 30044 | 55.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Escherichia coli O157:H7 GN=sucD PE=3 SV=2 | |||||
| 3 samesets of SUCD_ECO57 | |||||
| SUCD_ECOLI |
73 | 30044 | |||
| Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Escherichia coli (strain K12) GN=sucD PE=1 SV=2 | |||||
| SUCD_HAEIN |
73 | 30769 | |||
| Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Haemophilus influenzae GN=sucD PE=3 SV=1 | |||||
| SUCD_ECOL6 |
73 | 30044 | |||
| Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Escherichia coli O6 GN=sucD PE=3 SV=2 | |||||
56| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GUAA_PSEPG | 103 | GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas putida (strain GB-1) GN=guaA PE=3 SV=1 |
57| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_PSEPK | 102 | Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1 |
58| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYA_PSEPG | 102 | Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2 |
59| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTS_PSEPK | 102 | Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1 |
60| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TIG_PSEPK | 100 | Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2 |
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