MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 230)

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+51

Accession Score Description
1 RL14_PSE14 112 50S ribosomal protein L14 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplN PE=3 SV=1

-52

Accession Score Description
1 RL21_PSEE4 111 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1
Score Mass Matches Sequences emPAI
52.1 RL21_PSEE4 111 11596 7 (3) 4 (2) 0.76
50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1
4 samesets of RL21_PSEE4
RL21_PSEP1 111 11624 7 (3) 4 (2) 0.76
50S ribosomal protein L21 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplU PE=3 SV=1
RL21_PSEPG 111 11624 7 (3) 4 (2) 0.76
50S ribosomal protein L21 OS=Pseudomonas putida (strain GB-1) GN=rplU PE=3 SV=1
RL21_PSEPK 111 11624 7 (3) 4 (2) 0.76
50S ribosomal protein L21 OS=Pseudomonas putida (strain KT2440) GN=rplU PE=3 SV=2
RL21_PSEPW 111 11638 7 (3) 4 (2) 0.76
50S ribosomal protein L21 OS=Pseudomonas putida (strain W619) GN=rplU PE=3 SV=1

-7 peptide matches (6 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
300   602.3524 601.3451 601.3435 2.67 0 18 1.3 +4Score > 45 indicates identity
Score > 32 indicates homology		 K.ITGIQA.-
1051   951.4968 950.4895 950.4862 3.54 1 36 0.1 +1Score > 42 indicates identity
Score > 39 indicates homology		 R.QWFTEIK.I
1052 +1 476.2534 950.4922 950.4862 6.41 1 28 0.49 +1Score > 41 indicates identity
Score > 37 indicates homology		 R.QWFTEIK.I
1132   994.5598 993.5525 993.5495 3.04 0 87 6.7e-007 +1Score > 38 indicates identity U M.SYAVIVTGGK.Q
1133   497.7836 993.5526 993.5495 3.17 0 34 0.021 +1Score > 38 indicates identity
Score > 30 indicates homology		 U M.SYAVIVTGGK.Q
1239   522.2972 1042.5798 1042.5771 2.62 1 60 0.0003 +1Score > 40 indicates identity
Score > 37 indicates homology		 U K.VVAEVVSQGR.H

1 subset or intersection (3 subset proteins in total)

Score Mass Subset of
RL21_HAES1 60 11492 52.1
50S ribosomal protein L21 OS=Haemophilus somnus (strain 129Pt) GN=rplU PE=3 SV=1
2 samesets of RL21_HAES1
RL21_PSEMY 60 11605
50S ribosomal protein L21 OS=Pseudomonas mendocina (strain ymp) GN=rplU PE=3 SV=1
RL21_HAES2 60 11492
50S ribosomal protein L21 OS=Haemophilus somnus (strain 2336) GN=rplU PE=3 SV=1

+53

Accession Score Description
1 STHA_PSEPG 110 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1

+54

Accession Score Description
1 CARB_PSEPK 108 Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2

+55

Accession Score Description
1 SUCD_PSEAE 105 Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2

+56

Accession Score Description
1 GUAA_PSEPG 103 GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas putida (strain GB-1) GN=guaA PE=3 SV=1

+57

Accession Score Description
1 DNAK_PSEPK 102 Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1

+58

Accession Score Description
1 SYA_PSEPG 102 Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2

+59

Accession Score Description
1 EFTS_PSEPK 102 Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1

+60

Accession Score Description
1 TIG_PSEPK 100 Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2
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