MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 230)


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-51

Accession Score Description
1 RL14_PSE14 112 50S ribosomal protein L14 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplN PE=3 SV=1
Score Mass Matches Sequences emPAI
51.1 RL14_PSE14 112 13458 17 (6) 9 (4) 1.26
50S ribosomal protein L14 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplN PE=3 SV=1
13 samesets of RL14_PSE14
RL14_PSEF5 112 13471 17 (6) 9 (4) 1.26
50S ribosomal protein L14 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplN PE=3 SV=1
RL14_PSEFS 112 13458 17 (6) 9 (4) 1.26
50S ribosomal protein L14 OS=Pseudomonas fluorescens (strain SBW25) GN=rplN PE=3 SV=1
RL14_PSEMY 112 13444 17 (6) 9 (4) 1.26
50S ribosomal protein L14 OS=Pseudomonas mendocina (strain ymp) GN=rplN PE=3 SV=1
RL14_PSEP1 112 13458 17 (6) 9 (4) 1.26
50S ribosomal protein L14 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplN PE=3 SV=1
RL14_PSEPF 112 13458 17 (6) 9 (4) 1.26
50S ribosomal protein L14 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplN PE=3 SV=1
RL14_PSEPG 112 13458 17 (6) 9 (4) 1.26
50S ribosomal protein L14 OS=Pseudomonas putida (strain GB-1) GN=rplN PE=3 SV=1
RL14_PSEPK 112 13458 17 (6) 9 (4) 1.26
50S ribosomal protein L14 OS=Pseudomonas putida (strain KT2440) GN=rplN PE=3 SV=1
RL14_PSESM 112 13458 17 (6) 9 (4) 1.26
50S ribosomal protein L14 OS=Pseudomonas syringae pv. tomato GN=rplN PE=3 SV=1
RL14_PSEU2 112 13458 17 (6) 9 (4) 1.26
50S ribosomal protein L14 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplN PE=3 SV=1
RL14_PSEA8 112 13460 15 (6) 7 (4) 1.26
50S ribosomal protein L14 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplN PE=3 SV=1
RL14_PSEAB 112 13460 15 (6) 7 (4) 1.26
50S ribosomal protein L14 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplN PE=3 SV=1
RL14_PSEAE 112 13460 15 (6) 7 (4) 1.26
50S ribosomal protein L14 OS=Pseudomonas aeruginosa GN=rplN PE=3 SV=1
RL14_PSEU5 112 13484 15 (6) 7 (4) 1.26
50S ribosomal protein L14 OS=Pseudomonas stutzeri (strain A1501) GN=rplN PE=3 SV=1

-17 peptide matches (12 non-duplicate, 5 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
276   585.3362 584.3289 584.3282 1.26 1 18 0.42 +3Score > 37 indicates identity
Score > 27 indicates homology
K.EAIPR.G
535   366.2067 730.3988 730.3973 2.07 0 36 0.29 +1Score > 45 indicates identity
Score > 43 indicates homology
U R.ADGSIIR.F
654   789.4647 788.4574 788.4545 3.73 0 19 1.3 +3Score > 39 indicates identity
Score > 33 indicates homology
R.IFGPVTR.E
656 +2 395.2362 788.4578 788.4545 4.27 0 27 0.86 +1Score > 39 indicates identity
Score > 38 indicates homology
R.IFGPVTR.E
676   400.7178 799.4210 799.4188 2.80 0 32 0.28 +1Score > 39 indicates identity U K.QEPIGTR.I
878   444.2583 886.5020 886.4984 4.07 1 27 0.3 +1Score > 42 indicates identity
Score > 34 indicates homology
U R.RADGSIIR.F
1010 +1 940.5779 939.5706 939.5641 6.97 1 31 0.05 +1Score > 35 indicates identity
Score > 30 indicates homology
U K.IVSLAPEVL.-
1011   470.7928 939.5710 939.5641 7.42 1 26 0.016 +1Score > 35 indicates identity
Score > 21 indicates homology
U K.IVSLAPEVL.-
1045   949.5393 948.5320 948.5280 4.23 0 56 0.0012 +1Score > 39 indicates identity U R.YAGIGDIIK.V
1046 +1 475.2741 948.5336 948.5280 5.94 0 39 0.053 +1Score > 39 indicates identity U R.YAGIGDIIK.V
1266   530.3098 1058.6050 1058.5907 13.6 0 35 0.0089 +1Score > 39 indicates identity
Score > 27 indicates homology
U K.GQVMTAVVVR.T
1736 +1 638.3443 1274.6740 1274.6619 9.55 0 67 0.00015 +1Score > 41 indicates identity U R.FDGNAAVLLNNK.Q

6 subsets and intersections (513 subset proteins in total)

Score Mass Subset of
RL14_AZOSE 105 13343 51.1
50S ribosomal protein L14 OS=Azoarcus sp. (strain EbN1) GN=rplN PE=3 SV=1
9 samesets of RL14_AZOSE
RL14_DECAR 105 13371
50S ribosomal protein L14 OS=Dechloromonas aromatica (strain RCB) GN=rplN PE=3 SV=1
RL14_BURA4 105 13486
50S ribosomal protein L14 OS=Burkholderia ambifaria (strain MC40-6) GN=rplN PE=3 SV=1
RL14_BURCA 105 13454
50S ribosomal protein L14 OS=Burkholderia cenocepacia (strain AU 1054) GN=rplN PE=3 SV=2
RL14_BURCC 105 13454
50S ribosomal protein L14 OS=Burkholderia cenocepacia (strain MC0-3) GN=rplN PE=3 SV=1
RL14_BURCH 105 13454
50S ribosomal protein L14 OS=Burkholderia cenocepacia (strain HI2424) GN=rplN PE=3 SV=1
RL14_BURCJ 105 13454
50S ribosomal protein L14 OS=Burkholderia cepacia (strain J2315 / LMG 16656) GN=rplN PE=3 SV=1
RL14_BURCM 105 13486
50S ribosomal protein L14 OS=Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) GN=rplN PE=3 SV=1
RL14_BURS3 105 13454
50S ribosomal protein L14 OS=Burkholderia sp. (strain 383) GN=rplN PE=3 SV=1
RL14_BURVG 105 13486
50S ribosomal protein L14 OS=Burkholderia vietnamiensis (strain G4 / LMG 22486) GN=rplN PE=3 SV=1
RL14_BURM1 87 13502 51.1
50S ribosomal protein L14 OS=Burkholderia multivorans (strain ATCC 17616 / 249) GN=rplN PE=3 SV=2
+27 samesets of RL14_BURM1
RL14_PSEA7 60 13433 51.1
50S ribosomal protein L14 OS=Pseudomonas aeruginosa (strain PA7) GN=rplN PE=3 SV=1
2 samesets of RL14_PSEA7
RL14_PSEE4 60 13445
50S ribosomal protein L14 OS=Pseudomonas entomophila (strain L48) GN=rplN PE=3 SV=1
RL14_PSEPW 60 13445
50S ribosomal protein L14 OS=Pseudomonas putida (strain W619) GN=rplN PE=3 SV=1
RL14_ALCBS 56 13502 51.1
50S ribosomal protein L14 OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=rplN PE=3 SV=1
14 samesets of RL14_ALCBS
RL14_ALTMD 56 13389
50S ribosomal protein L14 OS=Alteromonas macleodii (strain DSM 17117 / Deep ecotype) GN=rplN PE=3 SV=1
RL14_BUCBP 56 13556
50S ribosomal protein L14 OS=Buchnera aphidicola subsp. Baizongia pistaciae GN=rplN PE=3 SV=1
RL14_EDWI9 56 13646
50S ribosomal protein L14 OS=Edwardsiella ictaluri (strain 93-146) GN=rplN PE=3 SV=1
RL14_YERE8 56 13688
50S ribosomal protein L14 OS=Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) GN=rplN PE=3 SV=1
RL14_YERP3 56 13688
50S ribosomal protein L14 OS=Yersinia pseudotuberculosis serotype O:1b (strain IP 31758) GN=rplN PE=3 SV=1
RL14_YERPA 56 13688
50S ribosomal protein L14 OS=Yersinia pestis bv. Antiqua (strain Antiqua) GN=rplN PE=3 SV=1
RL14_YERPB 56 13688
50S ribosomal protein L14 OS=Yersinia pseudotuberculosis serotype IB (strain PB1/+) GN=rplN PE=3 SV=1
RL14_YERPE 56 13688
50S ribosomal protein L14 OS=Yersinia pestis GN=rplN PE=3 SV=1
RL14_YERPG 56 13688
50S ribosomal protein L14 OS=Yersinia pestis bv. Antiqua (strain Angola) GN=rplN PE=3 SV=1
RL14_YERPN 56 13688
50S ribosomal protein L14 OS=Yersinia pestis bv. Antiqua (strain Nepal516) GN=rplN PE=3 SV=1
RL14_YERPP 56 13688
50S ribosomal protein L14 OS=Yersinia pestis (strain Pestoides F) GN=rplN PE=3 SV=1
RL14_YERPS 56 13688
50S ribosomal protein L14 OS=Yersinia pseudotuberculosis GN=rplN PE=3 SV=1
RL14_YERPY 56 13688
50S ribosomal protein L14 OS=Yersinia pseudotuberculosis serotype O:3 (strain YPIII) GN=rplN PE=3 SV=1
RL14_ALHEH 56 13477
50S ribosomal protein L14 OS=Alkalilimnicola ehrlichei (strain MLHE-1) GN=rplN PE=3 SV=1
RL14_AERHH 53 13451 51.1
50S ribosomal protein L14 OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) GN=rplN PE=3 SV=1
+26 samesets of RL14_AERHH
RK14_ACOCL 31 13643 51.1
50S ribosomal protein L14, chloroplastic OS=Acorus calamus GN=rpl14 PE=3 SV=1
+429 samesets of RK14_ACOCL

+52

Accession Score Description
1 RL21_PSEE4 111 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1

+53

Accession Score Description
1 STHA_PSEPG 110 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1

+54

Accession Score Description
1 CARB_PSEPK 108 Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2

+55

Accession Score Description
1 SUCD_PSEAE 105 Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2

+56

Accession Score Description
1 GUAA_PSEPG 103 GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas putida (strain GB-1) GN=guaA PE=3 SV=1

+57

Accession Score Description
1 DNAK_PSEPK 102 Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1

+58

Accession Score Description
1 SYA_PSEPG 102 Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2

+59

Accession Score Description
1 EFTS_PSEPK 102 Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1

+60

Accession Score Description
1 TIG_PSEPK 100 Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2
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