MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 230)

Page: 1 2 3 4 5 6 … 23 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 1648 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_LEGPA 425 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1599 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
5 EFTU_MYCS5 42 Elongation factor Tu OS=Mycoplasma synoviae (strain 53) GN=tuf PE=3 SV=1
3 EFTU_CARRP 146 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
4 EFTU_ANATD 54 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1484 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 907 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
3 ATPA_RICAH 176 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
2 ATPA_VEREI 178 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 PORF_PSESY 690 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1
2 PORF_PSEAE 143 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

-5

Accession Score Description
Family member distances as a dendrogram 1 RPOB_PSEPG 513 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
5 FTSZ_PSEPK 129 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOC_PSEP1 418 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoC PE=3 SV=1
3 RPOC_PSEU5 335 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas stutzeri (strain A1501) GN=rpoC PE=3 SV=1
6 RPOC_BIFLD 91 DNA-directed RNA polymerase subunit beta' OS=Bifidobacterium longum (strain DJO10A) GN=rpoC PE=3 SV=1
4 RPOC_SYNAS 202 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
RPOB_PSEPG 513 151555 34 (17) 29 (15) 0.29
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
3 samesets of RPOB_PSEPG
RPOB_PSEPK 513 151468 34 (17) 29 (15) 0.29
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1
RPOB_PSEP1 513 151468 34 (17) 29 (15) 0.29
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoB PE=3 SV=1
RPOB_PSEPW 513 151379 33 (17) 28 (15) 0.29
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain W619) GN=rpoB PE=3 SV=1
RPOC_PSEP1 418 155358 26 (16) 24 (16) 0.26
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoC PE=3 SV=1
1 sameset of RPOC_PSEP1
RPOC_PSEPK 418 155386 26 (16) 24 (16) 0.26
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1
RPOC_PSEU5 335 155574 20 (11) 18 (11) 0.17
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas stutzeri (strain A1501) GN=rpoC PE=3 SV=1
RPOC_SYNAS 202 154698 6 (4) 6 (4) 0.06
DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
FTSZ_PSEPK 129 41918 5 (4) 5 (4) 0.24
Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
RPOC_BIFLD 91 149913 4 (3) 4 (3) 0.05
DNA-directed RNA polymerase subunit beta' OS=Bifidobacterium longum (strain DJO10A) GN=rpoC PE=3 SV=1
1 sameset of RPOC_BIFLD
RPOC_BIFLO 91 149913 4 (3) 4 (3) 0.05
DNA-directed RNA polymerase subunit beta' OS=Bifidobacterium longum GN=rpoC PE=3 SV=1

-72 peptide matches (67 non-duplicate, 5 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 5 6 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 5 6 Peptide
228 +1 516.3041 515.2968 515.2955 2.60 0 10 1.5 +6Score > 47 indicates identity
Score > 25 indicates homology		 X R.ALAIE.K
424   339.6830 677.3514 677.3497 2.62 1 32 0.066 +1Score > 43 indicates identity
Score > 33 indicates homology		 X R.AGFEVR.D
461   347.7042 693.3938 693.3922 2.38 0 19 0.2 +2Score > 39 indicates identity
Score > 25 indicates homology		 X X X X R.APTLHR.L
483   352.2225 702.4304 702.4276 4.08 0 17 1.4 +4Score > 38 indicates identity
Score > 31 indicates homology		 U X R.GLATTIK.A
504   358.7194 715.4242 715.4228 1.97 0 37 0.16 +1Score > 43 indicates identity
Score > 41 indicates homology		 U X K.TLVDIR.N
515   361.7141 721.4136 721.4122 1.94 0 21 0.62 +1Score > 39 indicates identity
Score > 32 indicates homology		 X K.LLYSAR.I
544   367.7330 733.4514 733.4486 3.83 0 29 0.13 +1Score > 35 indicates identity
Score > 33 indicates homology		 U X K.VYLAIR.R
563   372.2564 742.4982 742.4953 4.01 0 31 0.072 +1Score > 32 indicates identity U X X R.VLLGITK.A
642 +1 392.7388 783.4630 783.4603 3.53 0 33 0.078 +1Score > 37 indicates identity
Score > 34 indicates homology		 X R.QAVPTLR.A
678   400.7366 799.4586 799.4552 4.31 1 16 0.83 +2Score > 42 indicates identity
Score > 28 indicates homology		 U X K.VIVEQGR.R
828   430.7469 859.4792 859.4763 3.37 0 22 1.8 +4Score > 42 indicates identity
Score > 37 indicates homology		 U X X R.IVDTTVGR.A
830   430.7499 859.4852 859.4803 5.70 0 37 0.16 +1Score > 41 indicates identity
Score > 41 indicates homology		 U X X K.VIDLWSK.A
842   436.2866 870.5586 870.5538 5.53 0 54 0.00043 +1Score > 33 indicates identity U X R.LLGVSALAK.Y
863   441.2482 880.4818 880.4766 5.92 1 22 0.71 +1Score > 38 indicates identity
Score > 33 indicates homology		 U X R.HINQLEK.A
948   458.7777 915.5408 915.5389 2.12 1 54 0.0016 +1Score > 39 indicates identity U X X X R.KGLADTALK.T
1000   470.2334 938.4522 938.4498 2.63 1 23 0.23 +1Score > 41 indicates identity
Score > 29 indicates homology		 U X X R.SWSFGEVK.K
1048   475.7526 949.4906 949.4869 3.97 1 35 0.2 +1Score > 42 indicates identity
Score > 40 indicates homology		 U X R.EFLQAGASK.D
1054   317.8635 950.5687 950.5661 2.66 0 33 0.068 +1Score > 34 indicates identity X R.VIVSQLHR.S
1055   318.5075 952.5007 952.4978 3.03 0 21 0.49 +1Score > 39 indicates identity
Score > 30 indicates homology		 X K.LNHLVDDK.M
1071   480.2906 958.5666 958.5600 6.92 0 35 0.021 +1Score > 37 indicates identity
Score > 30 indicates homology		 U X R.GVTFAVPLR.V
1125   494.7892 987.5638 987.5601 3.84 1 56 0.0012 +1Score > 39 indicates identity U X X R.VLTEAAVTGK.R
1125   494.7892 987.5638 987.5600 3.85 1 34 0.18 +4Score > 39 indicates identity U X R.VLTEASLAGK.V
1130   496.7619 991.5092 991.5087 0.57 0 25 0.21 +1Score > 41 indicates identity
Score > 31 indicates homology		 U X K.AQQYIVDR.R
1152   501.2749 1000.5352 1000.5302 5.09 0 36 0.0066 +1Score > 41 indicates identity
Score > 27 indicates homology		 U X R.ADGNLVAVSR.S
1166   503.2654 1004.5162 1004.5138 2.40 0 59 0.00016 +1Score > 42 indicates identity
Score > 34 indicates homology		 U X R.TSAADSVQVK.N
1169   503.7581 1005.5016 1005.4978 3.78 0 15 1.4 +2Score > 41 indicates identity
Score > 29 indicates homology		 U X R.TSAADSVQVK.N + Deamidated (NQ)
1184   339.5147 1015.5223 1015.5199 2.31 0 32 0.027 +1Score > 42 indicates identity
Score > 29 indicates homology		 U X X R.TFHIGGAASR.T
1202   514.3008 1026.5870 1026.5822 4.72 0 53 0.0024 +1Score > 39 indicates identity
Score > 39 indicates homology		 U X R.VSALGPGGLTR.E
1237   521.8186 1041.6226 1041.6183 4.22 0 30 0.08 +1Score > 38 indicates identity
Score > 32 indicates homology		 U X X R.SVITVGPTLR.L
1255   527.8199 1053.6252 1053.6182 6.67 1 47 0.0024 +1Score > 36 indicates identity
Score > 33 indicates homology		 U X K.LSLELVPQR.L
1265   530.2897 1058.5648 1058.5608 3.85 1 38 0.022 +1Score > 42 indicates identity
Score > 34 indicates homology		 U X R.IIGNATDEVK.E
1314   361.1786 1080.5140 1080.5101 3.58 0 28 0.43 +1Score > 41 indicates identity
Score > 37 indicates homology		 X R.DVHPTHYGR.V
1422   565.8429 1129.6712 1129.6707 0.50 0 27 0.048 +1Score > 35 indicates identity
Score > 26 indicates homology		 U X R.TILQLGTGVTK.G
1478   578.3442 1154.6738 1154.6659 6.88 1 51 0.0021 +1Score > 37 indicates identity
Score > 36 indicates homology		 U X K.QLIDELVAVR.H
1486 +1 578.8210 1155.6274 1155.6499 -19.4 1 50 0.0037 +1Score > 40 indicates identity
Score > 38 indicates homology		 U X K.QLIDELVAVR.H + Deamidated (NQ)
1539   392.8537 1175.5393 1175.5360 2.79 0 49 0.00038 +1Score > 41 indicates identity
Score > 27 indicates homology		 U X R.SPGVFFDHDR.G
1574   596.3060 1190.5974 1190.5932 3.61 1 48 0.0029 +1Score > 42 indicates identity
Score > 36 indicates homology		 U X X R.VFADLQEVDR.V
1667   617.8517 1233.6888 1233.6718 13.8 0 51 0.00013 +1Score > 38 indicates identity
Score > 24 indicates homology		 U X K.GTVIDVQVFTR.D
1806   657.8246 1313.6346 1313.6252 7.22 0 69 1.7e-005 +1Score > 40 indicates identity
Score > 34 indicates homology		 U X X X X R.FATSDLNDLYR.R
1828   441.8957 1322.6653 1322.6579 5.59 0 55 4.9e-005 +1Score > 41 indicates identity
Score > 24 indicates homology		 U X K.GIVDDIDHLGNR.R
1830   662.8994 1323.7842 1323.7762 6.09 0 63 6e-005 +1Score > 33 indicates identity U X X R.LLDLSAPDIIVR.N
1849   446.6024 1336.7854 1336.7755 7.42 1 52 0.00013 +1Score > 34 indicates identity
Score > 25 indicates homology		 U X X R.YKLPYGAVISVK.E
1873   677.3732 1352.7318 1352.7300 1.38 0 61 2.8e-005 +1Score > 38 indicates identity
Score > 28 indicates homology		 U X K.LQQGDDLAPGVLK.I
1943   693.3758 1384.7370 1384.7310 4.33 0 61 5.1e-005 +1Score > 40 indicates identity
Score > 30 indicates homology		 U X R.SALNGQVVDGGAGLK.K
2006   706.3684 1410.7222 1410.7143 5.63 1 82 3.5e-006 +1Score > 40 indicates identity U X X YIVNEIQDVYR.L
2059   720.3916 1438.7686 1438.7416 18.8 1 65 1.7e-005 +1Score > 39 indicates identity
Score > 30 indicates homology		 U X R.NPLLEDVNLQGAR.G + Deamidated (NQ)
2116   737.8554 1473.6962 1473.6888 5.03 1 63 0.00029 +1Score > 40 indicates identity U X R.TNQYGFLESPYR.V
2146   495.6019 1483.7839 1483.7783 3.75 1 70 6.3e-006 +1Score > 40 indicates identity
Score > 30 indicates homology		 U X X R.LIPAGTGLAYHSER.K
2230   757.4431 1512.8716 1512.8552 10.9 1 47 0.0033 +4Score > 34 indicates identity U X R.LGIQAFEPILVEGK.A
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_1_20250714114344.raw

Score > 39 indicates identity

Score > 19 indicates homology
-6.91 0 7 0.91 1 LLKPTVMMNLLGR   + Deamidated (NQ); 2 Oxidation (M)
14.1 0 6 1.1 2 LLVNITNDAWFGR  
-18.6 1 6 1.1 3 LLQTKNIMATGAIK   + Deamidated (NQ); Oxidation (M)
2236 +2 759.9175 1517.8204 1517.8453 -16.4 1 5 1.4 -4Score > 39 indicates identity
Score > 19 indicates homology		 U X R.LLGVSALARYIVNE.I + Deamidated (NQ)
1.82 0 4 1.9 5 LLMPPTWTGHPLR  
-7.36 0 3 2.1 6 MPNTVFIFLVPPK   + Oxidation (M)
-2.05 1 3 2.2 7 ILRSMIDTGIGGTGK  
16.8 1 3 2.2 8 QPNGPHLGIKATQR   + 2 Deamidated (NQ)
-8.98 0 3 2.3 9 VKPNLQTFNTILK   + 3 Deamidated (NQ)
-8.99 1 3 2.3 10 ILFPDIQIDTKSK   + Deamidated (NQ)
2332   393.9612 1571.8157 1571.8096 3.86 1 12 0.15 +1Score > 40 indicates identity
Score > 16 indicates homology		 U X K.WDPHTHPIVTELK.G
2455   811.9255 1621.8364 1621.8312 3.26 0 94 2e-008 +1Score > 40 indicates identity
Score > 30 indicates homology		 U X R.STGSYSLVTQQPLGGK.A
2619   565.6517 1693.9333 1693.9185 8.72 1 27 0.068 +1Score > 36 indicates identity
Score > 28 indicates homology		 U X R.MNVGQILETHLGLAAK.G
2642   569.6180 1705.8322 1705.8271 2.96 1 70 5.3e-006 +1Score > 41 indicates identity
Score > 30 indicates homology		 U X K.GEVISDGPSNPHDILR.L + Deamidated (NQ)
2642   569.6180 1705.8322 1705.8271 2.95 1 68 7.7e-006 +2Score > 41 indicates identity
Score > 30 indicates homology		 U X R.GEVISDGPSDPHDILR.L
2736   874.4435 1746.8724 1746.8611 6.51 1 90 6.6e-007 +1Score > 41 indicates identity U X K.LADLPESGQMVLFDGR.T
2803   889.4735 1776.9324 1776.9159 9.33 1 1 4 +7Score > 39 indicates identity
Score > 20 indicates homology		 U X R.EGLSVLQYFISTHGAR.K
2804   593.3192 1776.9358 1776.9159 11.2 1 52 0.00022 +1Score > 39 indicates identity
Score > 28 indicates homology		 U X R.EGLSVLQYFISTHGAR.K
3183   1009.5233 2017.0320 2017.0156 8.13 0 87 5e-007 +1Score > 39 indicates identity
Score > 36 indicates homology		 U X K.LNPQDDLDYLDIPAFLR.R
3211   1017.0008 2031.9870 2031.9749 5.98 1 114 1.8e-009 +1Score > 39 indicates identity
Score > 39 indicates homology		 U X K.ASLSTQSFISAASFQETTR.V + Deamidated (NQ)
3506   728.0535 2181.1387 2181.1066 14.7 1 87 6.3e-007 +1Score > 38 indicates identity U X K.QVVSVAASLIPFLEHDDANR.A + Deamidated (NQ)
3552   1103.5690 2205.1234 2205.1106 5.83 1 96 5.1e-008 +1Score > 38 indicates identity
Score > 35 indicates homology		 U X K.SVFPIISYSGNAALEYVGYR.L
3554   736.3828 2206.1266 2206.0946 14.5 1 36 0.0012 +1Score > 39 indicates identity
Score > 20 indicates homology		 U X K.SVFPIISYSGNAALEYVGYR.L + Deamidated (NQ)
3573   741.3990 2221.1752 2221.1340 18.5 0 42 0.0014 +1Score > 37 indicates identity
Score > 26 indicates homology		 U X K.LPDVMDVPYLLAIQLDSYR.E + Deamidated (NQ)
3791   791.4042 2371.1908 2371.1768 5.90 1 35 0.12 +1Score > 38 indicates identity U X K.VVDNTLQTAQQAYEASNPAPVR.Q

+57 subsets and intersections (904 subset proteins in total)

+6

Accession Score Description
1 RS5_PSEE4 428 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+7

Accession Score Description
1 RL3_PSEP1 378 50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1

+8

Accession Score Description
1 ARPC_PSEPU 369 Antibiotic efflux pump outer membrane protein arpC OS=Pseudomonas putida GN=arpC PE=2 SV=1

+9

Accession Score Description
1 RL2_PSEP1 329 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1

+10

Accession Score Description
Family member distances as a dendrogram 1 DLDH2_PSEPU 325 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4
2 DLDH_AZOVI 161 Dihydrolipoyl dehydrogenase OS=Azotobacter vinelandii PE=1 SV=1
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