MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 230)


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+41

Accession Score Description
1 RS4_PSEP1 144 30S ribosomal protein S4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsD PE=3 SV=1

+42

Accession Score Description
1 IF2_PSEE4 143 Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1

+43

Accession Score Description
Family member distances as a dendrogram 1 RL20_PSEE4 140 50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1
2 RL20_POLNS 61 50S ribosomal protein L20 OS=Polynucleobacter necessarius (strain STIR1) GN=rplT PE=3 SV=1

+44

Accession Score Description
1 ODO2_PSEPU 132 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (Fragment) OS=Pseudomonas putida GN=sucB PE=3 SV=2

-45

Accession Score Description
1 RL13_PSEP1 132 50S ribosomal protein L13 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplM PE=3 SV=1
Score Mass Matches Sequences emPAI
45.1 RL13_PSEP1 132 15852 11 (7) 7 (4) 1.00
50S ribosomal protein L13 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplM PE=3 SV=1
2 samesets of RL13_PSEP1
RL13_PSEPG 132 15852 11 (7) 7 (4) 1.00
50S ribosomal protein L13 OS=Pseudomonas putida (strain GB-1) GN=rplM PE=3 SV=1
RL13_PSEPK 132 15852 11 (7) 7 (4) 1.00
50S ribosomal protein L13 OS=Pseudomonas putida (strain KT2440) GN=rplM PE=3 SV=1

-11 peptide matches (8 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
257   556.3223 555.3150 555.3129 3.86 0 24 0.35 +1Score > 35 indicates identity
Score > 32 indicates homology
K.NPLGR.D
375   326.1716 650.3286 650.3275 1.74 1 9 7.9 +9Score > 43 indicates identity
Score > 31 indicates homology
E.INFEK.L + Deamidated (NQ)
586   759.4636 758.4563 758.4538 3.33 1 44 0.024 +1Score > 40 indicates identity U R.VIETAVK.G
850   874.5008 873.4935 873.4920 1.79 1 53 0.004 +1Score > 42 indicates identity U R.LATEIATR.L
852 +1 437.7545 873.4944 873.4920 2.85 1 42 0.05 +1Score > 42 indicates identity U R.LATEIATR.L
1400   374.5459 1120.6159 1120.6128 2.72 1 22 0.15 +1Score > 39 indicates identity
Score > 27 indicates homology
K.TFTAKPETVK.R
2284 +2 774.8990 1547.7834 1547.7733 6.59 1 64 4.6e-005 +1Score > 41 indicates identity
Score > 33 indicates homology
U R.EWFVVDAAGQTLGR.L
3072   487.5048 1945.9901 1945.9646 13.1 1 28 0.005 +1Score > 39 indicates identity
Score > 17 indicates homology
U K.VYAGAAHPHTAQQPQELK.I + Deamidated (NQ)

4 subsets and intersections (25 subset proteins in total)

Score Mass Subset of
RL13_PSEE4 118 15838 45.1
50S ribosomal protein L13 OS=Pseudomonas entomophila (strain L48) GN=rplM PE=3 SV=1
1 sameset of RL13_PSEE4
RL13_PSEPW 118 15852
50S ribosomal protein L13 OS=Pseudomonas putida (strain W619) GN=rplM PE=3 SV=1
RL13_COLP3 53 15993 45.1
50S ribosomal protein L13 OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) GN=rplM PE=3 SV=1
8 samesets of RL13_COLP3
RL13_PSYA2 53 15834
50S ribosomal protein L13 OS=Psychrobacter arcticus (strain DSM 17307 / 273-4) GN=rplM PE=3 SV=1
RL13_PSYCK 53 15701
50S ribosomal protein L13 OS=Psychrobacter cryohalolentis (strain K5) GN=rplM PE=3 SV=1
RL13_SHEB2 53 15728
50S ribosomal protein L13 OS=Shewanella baltica (strain OS223) GN=rplM PE=3 SV=1
RL13_SHEB5 53 15728
50S ribosomal protein L13 OS=Shewanella baltica (strain OS155 / ATCC BAA-1091) GN=rplM PE=3 SV=1
RL13_SHEB9 53 15728
50S ribosomal protein L13 OS=Shewanella baltica (strain OS195) GN=rplM PE=3 SV=1
RL13_SHEFN 53 15736
50S ribosomal protein L13 OS=Shewanella frigidimarina (strain NCIMB 400) GN=rplM PE=3 SV=1
RL13_SHEPC 53 15721
50S ribosomal protein L13 OS=Shewanella putrefaciens (strain CN-32 / ATCC BAA-453) GN=rplM PE=3 SV=1
RL13_SHESW 53 15721
50S ribosomal protein L13 OS=Shewanella sp. (strain W3-18-1) GN=rplM PE=3 SV=1
RL13_AZOVD 44 15838 45.1
50S ribosomal protein L13 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplM PE=3 SV=1
11 samesets of RL13_AZOVD
RL13_PSEMY 44 15890
50S ribosomal protein L13 OS=Pseudomonas mendocina (strain ymp) GN=rplM PE=3 SV=1
RL13_PSE14 44 15868
50S ribosomal protein L13 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplM PE=3 SV=1
RL13_PSESM 44 15853
50S ribosomal protein L13 OS=Pseudomonas syringae pv. tomato GN=rplM PE=3 SV=1
RL13_PSEU2 44 15868
50S ribosomal protein L13 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplM PE=3 SV=1
RL13_PSEU5 44 15896
50S ribosomal protein L13 OS=Pseudomonas stutzeri (strain A1501) GN=rplM PE=3 SV=1
RL13_HAHCH 44 16147
50S ribosomal protein L13 OS=Hahella chejuensis (strain KCTC 2396) GN=rplM PE=3 SV=1
RL13_PSEA7 44 16046
50S ribosomal protein L13 OS=Pseudomonas aeruginosa (strain PA7) GN=rplM PE=3 SV=1
RL13_PSEA8 44 16018
50S ribosomal protein L13 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplM PE=3 SV=1
RL13_PSEAB 44 16018
50S ribosomal protein L13 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplM PE=3 SV=1
RL13_PSEAE 44 16018
50S ribosomal protein L13 OS=Pseudomonas aeruginosa GN=rplM PE=3 SV=1
RL13_STRM5 44 16204
50S ribosomal protein L13 OS=Stenotrophomonas maltophilia (strain R551-3) GN=rplM PE=3 SV=1
RL13_PSEF5 43 15810 45.1
50S ribosomal protein L13 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplM PE=3 SV=1
1 sameset of RL13_PSEF5
RL13_PSEPF 43 15824
50S ribosomal protein L13 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplM PE=3 SV=1

+46

Accession Score Description
1 EXBB_PSEPU 129 Biopolymer transport protein exbB OS=Pseudomonas putida GN=exbB PE=3 SV=1

+47

Accession Score Description
1 PCXB_PSEPU 126 Protocatechuate 3,4-dioxygenase beta chain OS=Pseudomonas putida GN=pcaH PE=1 SV=3

+48

Accession Score Description
1 AMPA_PSEPG 123 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1

+49

Accession Score Description
1 METN1_PSEPK 122 Methionine import ATP-binding protein metN 1 OS=Pseudomonas putida (strain KT2440) GN=metN1 PE=3 SV=1

+50

Accession Score Description
1 ACON2_PSEAE 113 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1
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