MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 sp |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 22:50:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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41| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS4_PSEP1 | 144 | 30S ribosomal protein S4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsD PE=3 SV=1 |
42| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IF2_PSEE4 | 143 | Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1 |
43| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL20_PSEE4 | 140 | 50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1 | |
| 2 | RL20_POLNS | 61 | 50S ribosomal protein L20 OS=Polynucleobacter necessarius (strain STIR1) GN=rplT PE=3 SV=1 | |
44| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODO2_PSEPU | 132 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (Fragment) OS=Pseudomonas putida GN=sucB PE=3 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 44.1 | ODO2_PSEPU |
132 | 6687 | 12 (4) | 4 (1) | 0.38 | |
| Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (Fragment) OS=Pseudomonas putida GN=sucB PE=3 SV=2 | |||||||
12 peptide matches (5 non-duplicate, 7 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 252 |
545.3285 | 544.3212 | 544.3221 | -1.52 | 0 | 20 | 0.69 | 1Score > 43 indicates identity Score > 31 indicates homology |
R.LIDGK.E | ||
| 283 |
4 |
586.3860 | 585.3787 | 585.3737 | 8.50 | 0 | 25 | 0.58 | 1Score > 39 indicates identity Score > 35 indicates homology |
U | R.LLLDI.- |
| 1024 |
943.4783 | 942.4710 | 942.4770 | -6.38 | 1 | 19 | 5.6 | 8Score > 39 indicates identity |
U | K.NLLEDPSR.L | |
| 1025 |
472.2466 | 942.4786 | 942.4770 | 1.71 | 1 | 25 | 0.19 | 1Score > 39 indicates identity Score > 30 indicates homology |
U | K.NLLEDPSR.L | |
| 1397 |
3 |
560.8389 | 1119.6632 | 1119.6540 | 8.29 | 1 | 68 | 1e-005 | 1Score > 32 indicates identity Score > 31 indicates homology |
U | K.EAVTFLVTIK.N |
45| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL13_PSEP1 | 132 | 50S ribosomal protein L13 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplM PE=3 SV=1 |
46| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EXBB_PSEPU | 129 | Biopolymer transport protein exbB OS=Pseudomonas putida GN=exbB PE=3 SV=1 |
47| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PCXB_PSEPU | 126 | Protocatechuate 3,4-dioxygenase beta chain OS=Pseudomonas putida GN=pcaH PE=1 SV=3 |
48| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_PSEPG | 123 | Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1 |
49| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | METN1_PSEPK | 122 | Methionine import ATP-binding protein metN 1 OS=Pseudomonas putida (strain KT2440) GN=metN1 PE=3 SV=1 |
50| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACON2_PSEAE | 113 | Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1 |
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