MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 230)


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+41

Accession Score Description
1 RS4_PSEP1 144 30S ribosomal protein S4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsD PE=3 SV=1

+42

Accession Score Description
1 IF2_PSEE4 143 Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1

-43

Accession Score Description
Family member distances as a dendrogram 1 RL20_PSEE4 140 50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1
2 RL20_POLNS 61 50S ribosomal protein L20 OS=Polynucleobacter necessarius (strain STIR1) GN=rplT PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
43.1 RL20_PSEE4 140 13272 20 (9) 10 (5) 1.69
50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1
4 samesets of RL20_PSEE4
RL20_PSEP1 140 13272 20 (9) 10 (5) 1.69
50S ribosomal protein L20 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplT PE=3 SV=1
RL20_PSEPG 140 13272 20 (9) 10 (5) 1.69
50S ribosomal protein L20 OS=Pseudomonas putida (strain GB-1) GN=rplT PE=3 SV=1
RL20_PSEPK 140 13272 20 (9) 10 (5) 1.69
50S ribosomal protein L20 OS=Pseudomonas putida (strain KT2440) GN=rplT PE=3 SV=1
RL20_PSEPW 140 13272 20 (9) 10 (5) 1.69
50S ribosomal protein L20 OS=Pseudomonas putida (strain W619) GN=rplT PE=3 SV=1
43.2 RL20_POLNS 61 13738 7 (5) 3 (2) 0.61
50S ribosomal protein L20 OS=Polynucleobacter necessarius (strain STIR1) GN=rplT PE=3 SV=1
1 sameset of RL20_POLNS
RL20_POLSQ 61 13742 7 (5) 3 (2) 0.61
50S ribosomal protein L20 OS=Polynucleobacter sp. (strain QLW-P1DMWA-1) GN=rplT PE=3 SV=1

+23 peptide matches (15 non-duplicate, 8 duplicate)

9 subsets and intersections (257 subset proteins in total)

Score Mass Subset of
RL20_PSEF5 127 13287 43.1
50S ribosomal protein L20 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplT PE=3 SV=1
3 samesets of RL20_PSEF5
RL20_PSEFL 127 13287
50S ribosomal protein L20 OS=Pseudomonas fluorescens GN=rplT PE=3 SV=1
RL20_PSEMY 127 13241
50S ribosomal protein L20 OS=Pseudomonas mendocina (strain ymp) GN=rplT PE=3 SV=1
RL20_PSEU5 127 13284
50S ribosomal protein L20 OS=Pseudomonas stutzeri (strain A1501) GN=rplT PE=3 SV=1
RL20_PSE14 121 13306 43.1
50S ribosomal protein L20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplT PE=3 SV=1
4 samesets of RL20_PSE14
RL20_PSEPF 121 13306
50S ribosomal protein L20 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplT PE=3 SV=1
RL20_PSESM 121 13306
50S ribosomal protein L20 OS=Pseudomonas syringae pv. tomato GN=rplT PE=3 SV=1
RL20_PSESY 121 13306
50S ribosomal protein L20 OS=Pseudomonas syringae pv. syringae GN=rplT PE=3 SV=1
RL20_PSEU2 121 13306
50S ribosomal protein L20 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplT PE=3 SV=1
RL20_PSEA7 93 13357 43.1
50S ribosomal protein L20 OS=Pseudomonas aeruginosa (strain PA7) GN=rplT PE=3 SV=1
3 samesets of RL20_PSEA7
RL20_PSEA8 93 13357
50S ribosomal protein L20 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplT PE=3 SV=1
RL20_PSEAB 93 13357
50S ribosomal protein L20 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplT PE=3 SV=1
RL20_PSEAE 93 13357
50S ribosomal protein L20 OS=Pseudomonas aeruginosa GN=rplT PE=3 SV=1
RL20_AZOVD 81 13385 43.1
50S ribosomal protein L20 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplT PE=3 SV=1
1 sameset of RL20_AZOVD
RL20_AZOVI 81 13429
50S ribosomal protein L20 OS=Azotobacter vinelandii GN=rplT PE=3 SV=1
RL20_PROMH 74 13449 43.1
50S ribosomal protein L20 OS=Proteus mirabilis (strain HI4320) GN=rplT PE=3 SV=1
RL20_ERWT9 51 13490 43.1
50S ribosomal protein L20 OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=rplT PE=3 SV=1
RL20_MARAV 45 13234 43.1
50S ribosomal protein L20 OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=rplT PE=3 SV=1
2 samesets of RL20_MARAV
RL20_SACD2 45 13355
50S ribosomal protein L20 OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rplT PE=3 SV=1
RL20_TERTT 45 13373
50S ribosomal protein L20 OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=rplT PE=3 SV=1
RL20_ACIAC 41 13531 43.1
50S ribosomal protein L20 OS=Acidovorax avenae subsp. citrulli (strain AAC00-1) GN=rplT PE=3 SV=1
+230 samesets of RL20_ACIAC
ARLY_LACRD 38 52058 43.1
Argininosuccinate lyase OS=Lactobacillus reuteri (strain DSM 20016) GN=argH PE=3 SV=1
5 samesets of ARLY_LACRD
ARLY_LACRJ 38 52058
Argininosuccinate lyase OS=Lactobacillus reuteri (strain JCM 1112) GN=argH PE=3 SV=1
ORN_PHOLL 38 21012
Oligoribonuclease OS=Photorhabdus luminescens subsp. laumondii GN=orn PE=3 SV=1
SRYC_DROME 38 96737
Serendipity locus protein H-1 OS=Drosophila melanogaster GN=wdn PE=1 SV=2
SYR_DESMR 38 60602
Arginyl-tRNA synthetase OS=Desulfovibrio magneticus (strain ATCC 700980 / DSM 13731 / RS-1) GN=argS PE=3 SV=1
RL20_ANADF 38 13101
50S ribosomal protein L20 OS=Anaeromyxobacter sp. (strain Fw109-5) GN=rplT PE=3 SV=1

+44

Accession Score Description
1 ODO2_PSEPU 132 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (Fragment) OS=Pseudomonas putida GN=sucB PE=3 SV=2

+45

Accession Score Description
1 RL13_PSEP1 132 50S ribosomal protein L13 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplM PE=3 SV=1

+46

Accession Score Description
1 EXBB_PSEPU 129 Biopolymer transport protein exbB OS=Pseudomonas putida GN=exbB PE=3 SV=1

+47

Accession Score Description
1 PCXB_PSEPU 126 Protocatechuate 3,4-dioxygenase beta chain OS=Pseudomonas putida GN=pcaH PE=1 SV=3

+48

Accession Score Description
1 AMPA_PSEPG 123 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1

+49

Accession Score Description
1 METN1_PSEPK 122 Methionine import ATP-binding protein metN 1 OS=Pseudomonas putida (strain KT2440) GN=metN1 PE=3 SV=1

+50

Accession Score Description
1 ACON2_PSEAE 113 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1
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