MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 sp |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 22:50:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
41| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS4_PSEP1 | 144 | 30S ribosomal protein S4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsD PE=3 SV=1 |
42| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IF2_PSEE4 | 143 | Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 42.1 | IF2_PSEE4 |
143 | 91973 | 6 (3) | 6 (3) | 0.08 | |
| Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1 | |||||||
| 4 samesets of IF2_PSEE4 | |||||||
| IF2_PSEP1 |
143 | 91620 | 5 (3) | 5 (3) | 0.08 | ||
| Translation initiation factor IF-2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=infB PE=3 SV=1 | |||||||
| IF2_PSEPG |
143 | 91294 | 5 (3) | 5 (3) | 0.08 | ||
| Translation initiation factor IF-2 OS=Pseudomonas putida (strain GB-1) GN=infB PE=3 SV=1 | |||||||
| IF2_PSEPK |
143 | 91576 | 5 (3) | 5 (3) | 0.08 | ||
| Translation initiation factor IF-2 OS=Pseudomonas putida (strain KT2440) GN=infB PE=3 SV=1 | |||||||
| IF2_PSEPW |
143 | 91289 | 5 (3) | 5 (3) | 0.08 | ||
| Translation initiation factor IF-2 OS=Pseudomonas putida (strain W619) GN=infB PE=3 SV=1 | |||||||
6 peptide matches (6 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 682 |
400.7674 | 799.5202 | 799.5167 | 4.41 | 0 | 20 | 0.91 | 1Score > 36 indicates identity Score > 32 indicates homology |
U | K.TLNIVLK.T | |
| 1114 |
490.7776 | 979.5406 | 979.5338 | 6.96 | 0 | 39 | 0.048 | 1Score > 39 indicates identity |
U | K.TSLLDYIR.R | |
| 2060 |
720.4216 | 1438.8286 | 1438.8144 | 9.91 | 0 | 73 | 1.1e-005 | 1Score > 36 indicates identity |
U | R.GPVATILVQDGTLR.Q | |
| 2317 |
522.2715 | 1563.7927 | 1563.7906 | 1.30 | 0 | 23 | 0.54 | 1Score > 41 indicates identity Score > 33 indicates homology |
U | R.NQHGFQNPTGPVIR.D | |
| 2795 |
887.4505 | 1772.8864 | 1772.8904 | -2.25 | 1 | 4 | 1.4 | 4Score > 40 indicates identity Score > 18 indicates homology |
U | E.ALQGSLSSLGNDEVQVR.V + Deamidated (NQ) | |
| 4272 |
973.8608 | 2918.5606 | 2918.5138 | 16.0 | 1 | 98 | 3.1e-009 | 1Score > 34 indicates identity Score > 25 indicates homology |
U | R.VIGGGVGGITESDANLALASNAVLFGFNVR.A + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| IF2_CAMC5 |
74 | 99413 | 42.1 | ||
| Translation initiation factor IF-2 OS=Campylobacter curvus (strain 525.92) GN=infB PE=3 SV=1 | |||||
| 2 samesets of IF2_CAMC5 | |||||
| IF2_CAMHC |
74 | 100767 | |||
| Translation initiation factor IF-2 OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=infB PE=3 SV=1 | |||||
| IF2_CAMLR |
74 | 96725 | |||
| Translation initiation factor IF-2 OS=Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060) GN=infB PE=3 SV=1 | |||||
| IF2_CYAP4 |
73 | 108559 | 42.1 | ||
| Translation initiation factor IF-2 OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN=infB PE=3 SV=1 | |||||
| 10 samesets of IF2_CYAP4 | |||||
| IF2_MICAN |
73 | 109614 | |||
| Translation initiation factor IF-2 OS=Microcystis aeruginosa (strain NIES-843) GN=infB PE=3 SV=1 | |||||
| IF2_SYNE7 |
73 | 110858 | |||
| Translation initiation factor IF-2 OS=Synechococcus elongatus (strain PCC 7942) GN=infB PE=3 SV=1 | |||||
| IF2_SYNJA |
73 | 111852 | |||
| Translation initiation factor IF-2 OS=Synechococcus sp. (strain JA-3-3Ab) GN=infB PE=3 SV=1 | |||||
| IF2_SYNJB |
73 | 113485 | |||
| Translation initiation factor IF-2 OS=Synechococcus sp. GN=infB PE=3 SV=1 | |||||
| IF2_SYNP6 |
73 | 110787 | |||
| Translation initiation factor IF-2 OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=infB PE=3 SV=1 | |||||
| IF2_CYAP7 |
73 | 121749 | |||
| Translation initiation factor IF-2 OS=Cyanothece sp. (strain PCC 7424) GN=infB PE=3 SV=1 | |||||
| IF2_THEEB |
73 | 104469 | |||
| Translation initiation factor IF-2 OS=Thermosynechococcus elongatus (strain BP-1) GN=infB PE=3 SV=1 | |||||
| IF2_SYNP2 |
73 | 105884 | |||
| Translation initiation factor IF-2 OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) GN=infB PE=3 SV=1 | |||||
| IF2_SYNPW |
73 | 119022 | |||
| Translation initiation factor IF-2 OS=Synechococcus sp. (strain WH7803) GN=infB PE=3 SV=1 | |||||
| IF2_SYNS3 |
73 | 118793 | |||
| Translation initiation factor IF-2 OS=Synechococcus sp. (strain CC9311) GN=infB PE=3 SV=1 | |||||
| IF2C_EUGGR |
39 | 88047 | 42.1 | ||
| Translation initiation factor IF-2, chloroplastic (Fragment) OS=Euglena gracilis GN=INFB PE=2 SV=1 | |||||
| 252 samesets of IF2C_EUGGR |
|||||
43| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL20_PSEE4 | 140 | 50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1 | |
| 2 | RL20_POLNS | 61 | 50S ribosomal protein L20 OS=Polynucleobacter necessarius (strain STIR1) GN=rplT PE=3 SV=1 | |
44| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODO2_PSEPU | 132 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (Fragment) OS=Pseudomonas putida GN=sucB PE=3 SV=2 |
45| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL13_PSEP1 | 132 | 50S ribosomal protein L13 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplM PE=3 SV=1 |
46| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EXBB_PSEPU | 129 | Biopolymer transport protein exbB OS=Pseudomonas putida GN=exbB PE=3 SV=1 |
47| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PCXB_PSEPU | 126 | Protocatechuate 3,4-dioxygenase beta chain OS=Pseudomonas putida GN=pcaH PE=1 SV=3 |
48| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_PSEPG | 123 | Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1 |
49| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | METN1_PSEPK | 122 | Methionine import ATP-binding protein metN 1 OS=Pseudomonas putida (strain KT2440) GN=metN1 PE=3 SV=1 |
50| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACON2_PSEAE | 113 | Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1 |
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