MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 sp |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 22:50:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPG | 1648 | ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1 | |
| 2 | ATPB_LEGPA | 425 | ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 1599 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 5 | EFTU_MYCS5 | 42 | Elongation factor Tu OS=Mycoplasma synoviae (strain 53) GN=tuf PE=3 SV=1 | |
| 3 | EFTU_CARRP | 146 | Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1 | |
| 4 | EFTU_ANATD | 54 | Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 1484 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPK | 907 | ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1 | |
| 3 | ATPA_RICAH | 176 | ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1 | |
| 2 | ATPA_VEREI | 178 | ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSESY | 690 | Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1 | |
| 2 | PORF_PSEAE | 143 | Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 | |
29 peptide matches (11 non-duplicate, 18 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | 1 | 2 | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | 1 | 2 | Peptide |
| 400 |
2 |
336.1563 | 670.2980 | 670.2962 | 2.68 | 0 | 14 | 0.98 | 1Score > 37 indicates identity Score > 26 indicates homology |
|
|
K.FDFDK.S | |
| 402 |
671.3054 | 670.2981 | 670.2962 | 2.80 | 0 | 27 | 0.091 | 1Score > 37 indicates identity Score > 29 indicates homology |
|
|
K.FDFDK.S | ||
| 476 |
702.4055 | 701.3982 | 701.3959 | 3.24 | 1 | 46 | 0.033 | 1Score > 43 indicates identity |
U | |
|
R.VeLDVK.F | |
| 477 |
1 |
351.7064 | 701.3982 | 701.3959 | 3.28 | 1 | 34 | 0.37 | 1Score > 43 indicates identity Score > 42 indicates homology |
U | |
|
R.VeLDVK.F |
| 755 |
838.4151 | 837.4078 | 837.4055 | 2.83 | 0 | 46 | 0.016 | 1Score > 40 indicates identity |
U | |
K.NLADFMK.Q | ||
| 757 |
2 |
419.7119 | 837.4092 | 837.4055 | 4.53 | 0 | 38 | 0.0072 | 1Score > 40 indicates identity Score > 29 indicates homology |
U | |
K.NLADFMK.Q | |
| 1793 |
2 |
436.2396 | 1305.6970 | 1305.6929 | 3.15 | 0 | 53 | 0.00027 | 1Score > 40 indicates identity Score > 30 indicates homology |
U | |
K.SVVKPNSYGDIK.N | |
| 1794 |
1 |
653.8558 | 1305.6970 | 1305.6929 | 3.21 | 0 | 66 | 8.7e-005 | 1Score > 40 indicates identity Score > 38 indicates homology |
U | |
K.SVVKPNSYGDIK.N | |
| 1875 |
2 |
452.2384 | 1353.6934 | 1353.6816 | 8.67 | 1 | 64 | 4e-005 | 1Score > 40 indicates identity Score > 33 indicates homology |
U | |
R.VQLDVKFDFDK.S + Deamidated (NQ) | |
| 3694 |
6 |
1152.0616 | 2302.1086 | 2302.0753 | 14.5 | 1 | 122 | 1.1e-010 | 1Score > 39 indicates identity Score > 35 indicates homology |
U | |
K.NNGNLFGGSIGYFLTDDVELR.L + 2 Deamidated (NQ) | |
| 3698 |
2 |
768.3794 | 2302.1164 | 2302.0753 | 17.8 | 1 | 93 | 2.3e-007 | 1Score > 39 indicates identity |
U | |
K.NNGNLFGGSIGYFLTDDVELR.L + 2 Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| PORF_PSEFL |
46 | 34461 | 4.1 | ||
| Outer membrane porin F OS=Pseudomonas fluorescens GN=oprF PE=1 SV=1 | |||||
| 1 sameset of PORF_PSEFL | |||||
| THIC_THELT |
46 | 47776 | |||
| Phosphomethylpyrimidine synthase OS=Thermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / TMO) GN=thiC PE=3 SV=1 | |||||
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_PSEPG | 513 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1 | |
| 5 | FTSZ_PSEPK | 129 | Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3 | |
| 2 | RPOC_PSEP1 | 418 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoC PE=3 SV=1 | |
| 3 | RPOC_PSEU5 | 335 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas stutzeri (strain A1501) GN=rpoC PE=3 SV=1 | |
| 6 | RPOC_BIFLD | 91 | DNA-directed RNA polymerase subunit beta' OS=Bifidobacterium longum (strain DJO10A) GN=rpoC PE=3 SV=1 | |
| 4 | RPOC_SYNAS | 202 | DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1 | |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEE4 | 428 | 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL3_PSEP1 | 378 | 50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARPC_PSEPU | 369 | Antibiotic efflux pump outer membrane protein arpC OS=Pseudomonas putida GN=arpC PE=2 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL2_PSEP1 | 329 | 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DLDH2_PSEPU | 325 | Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4 | |
| 2 | DLDH_AZOVI | 161 | Dihydrolipoyl dehydrogenase OS=Azotobacter vinelandii PE=1 SV=1 | |
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