MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 230)


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+31

Accession Score Description
1 OXAA_PSEPK 175 Inner membrane protein oxaA OS=Pseudomonas putida (strain KT2440) GN=oxaA PE=3 SV=1

+32

Accession Score Description
Family member distances as a dendrogram 1 RS3_PSEE4 173 30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1
2 RS3_NITOC 80 30S ribosomal protein S3 OS=Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) GN=rpsC PE=3 SV=1

+33

Accession Score Description
1 ODO1_AZOVI 172 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

+34

Accession Score Description
1 RS11_PSEE4 169 30S ribosomal protein S11 OS=Pseudomonas entomophila (strain L48) GN=rpsK PE=3 SV=1

+35

Accession Score Description
Family member distances as a dendrogram 1 RS9_PSEE4 161 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1
2 RS9_PSE14 90 30S ribosomal protein S9 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsI PE=3 SV=1

+36

Accession Score Description
1 RISB_PSEPG 159 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

+37

Accession Score Description
1 CYOA_PSEPU 155 Ubiquinol oxidase subunit 2 OS=Pseudomonas putida GN=cyoA PE=3 SV=1

+38

Accession Score Description
1 AROG_ECOLI 155 Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive OS=Escherichia coli (strain K12) GN=aroG PE=1 SV=1

-39

Accession Score Description
1 RL28_PSEP1 150 50S ribosomal protein L28 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpmB PE=3 SV=1
Score Mass Matches Sequences emPAI
39.1 RL28_PSEP1 150 8974 6 (4) 4 (2) 1.06
50S ribosomal protein L28 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpmB PE=3 SV=1
3 samesets of RL28_PSEP1
RL28_PSEPG 150 8974 6 (4) 4 (2) 1.06
50S ribosomal protein L28 OS=Pseudomonas putida (strain GB-1) GN=rpmB PE=3 SV=1
RL28_PSEPK 150 8974 6 (4) 4 (2) 1.06
50S ribosomal protein L28 OS=Pseudomonas putida (strain KT2440) GN=rpmB PE=3 SV=1
RL28_PSEE4 150 8988 5 (4) 3 (2) 1.06
50S ribosomal protein L28 OS=Pseudomonas entomophila (strain L48) GN=rpmB PE=3 SV=1

-6 peptide matches (5 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1286 +1 535.8203 1069.6260 1069.6132 12.1 0 48 0.0052 +1Score > 38 indicates identity U R.GIDAVLVDIR.K
1507   387.8816 1160.6230 1160.6203 2.30 0 7 1.4 +7Score > 41 indicates identity
Score > 21 indicates homology
R.FLPNLQHHR.F
1590   400.2456 1197.7150 1197.7081 5.73 1 28 0.34 +1Score > 36 indicates identity
Score > 35 indicates homology
U R.GIDAVLVDIRK.A
2024   474.9090 1421.7052 1421.7011 2.85 0 69 1.9e-006 +1Score > 41 indicates identity
Score > 25 indicates homology
U K.GPVTGNNISHANNK.T
2025   711.8599 1421.7052 1421.7011 2.90 0 97 5.3e-008 +1Score > 41 indicates identity
Score > 37 indicates homology
U K.GPVTGNNISHANNK.T

2 subsets and intersections (11 subset proteins in total)

Score Mass Subset of
RL28_AZOVD 135 9074 39.1
50S ribosomal protein L28 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpmB PE=3 SV=1
9 samesets of RL28_AZOVD
RL28_PSE14 135 8987
50S ribosomal protein L28 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmB PE=3 SV=1
RL28_PSEF5 135 9046
50S ribosomal protein L28 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpmB PE=3 SV=1
RL28_PSEFS 135 8902
50S ribosomal protein L28 OS=Pseudomonas fluorescens (strain SBW25) GN=rpmB PE=3 SV=1
RL28_PSEMY 135 9060
50S ribosomal protein L28 OS=Pseudomonas mendocina (strain ymp) GN=rpmB PE=3 SV=1
RL28_PSEPF 135 9003
50S ribosomal protein L28 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpmB PE=3 SV=1
RL28_PSEPW 135 9094
50S ribosomal protein L28 OS=Pseudomonas putida (strain W619) GN=rpmB PE=3 SV=1
RL28_PSESM 135 8987
50S ribosomal protein L28 OS=Pseudomonas syringae pv. tomato GN=rpmB PE=3 SV=1
RL28_PSEU2 135 8987
50S ribosomal protein L28 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpmB PE=3 SV=1
RL28_PSEU5 135 9060
50S ribosomal protein L28 OS=Pseudomonas stutzeri (strain A1501) GN=rpmB PE=3 SV=1
RL28_VIBSL 49 9081 39.1
50S ribosomal protein L28 OS=Vibrio splendidus (strain LGP32) GN=rpmB PE=3 SV=1

+40

Accession Score Description
1 DBHB_PSEAE 148 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3
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