MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 230)

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+31

Accession Score Description
1 OXAA_PSEPK 175 Inner membrane protein oxaA OS=Pseudomonas putida (strain KT2440) GN=oxaA PE=3 SV=1

+32

Accession Score Description
Family member distances as a dendrogram 1 RS3_PSEE4 173 30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1
2 RS3_NITOC 80 30S ribosomal protein S3 OS=Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) GN=rpsC PE=3 SV=1

+33

Accession Score Description
1 ODO1_AZOVI 172 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

+34

Accession Score Description
1 RS11_PSEE4 169 30S ribosomal protein S11 OS=Pseudomonas entomophila (strain L48) GN=rpsK PE=3 SV=1

+35

Accession Score Description
Family member distances as a dendrogram 1 RS9_PSEE4 161 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1
2 RS9_PSE14 90 30S ribosomal protein S9 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsI PE=3 SV=1

-36

Accession Score Description
1 RISB_PSEPG 159 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1
Score Mass Matches Sequences emPAI
36.1 RISB_PSEPG 159 16467 8 (6) 5 (4) 0.96
6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1
3 samesets of RISB_PSEPG
RISB_PSEPK 159 16467 8 (6) 5 (4) 0.96
6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain KT2440) GN=ribH PE=3 SV=1
RISB_PSEP1 159 16467 8 (6) 5 (4) 0.96
6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ribH PE=3 SV=1
RISB_PSEE4 159 16467 8 (6) 5 (4) 0.96
6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas entomophila (strain L48) GN=ribH PE=3 SV=1

-8 peptide matches (7 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
627   389.2352 776.4558 776.4545 1.78 0 29 0.14 +1Score > 39 indicates identity
Score > 33 indicates homology		 R.YALVVGR.F
1306   538.8058 1075.5970 1075.5914 5.29 1 32 0.034 +1Score > 40 indicates identity
Score > 30 indicates homology		 U K.TIEGTFIAPK.G
1832   442.9068 1325.6986 1325.6939 3.51 1 17 0.1 +1Score > 40 indicates identity
Score > 20 indicates homology		 U R.HGVSESDITIIR.A
1833   663.8619 1325.7092 1325.6939 11.6 1 34 0.011 +1Score > 39 indicates identity
Score > 27 indicates homology		 U R.HGVSESDITIIR.A
1853 +1 670.8918 1339.7690 1339.7500 14.2 1 60 1.2e-005 +1Score > 36 indicates identity
Score > 24 indicates homology		 U R.APGAFEIPLVAQK.V
2902   915.0229 1828.0312 1828.0207 5.79 0 82 1.3e-007 +1Score > 34 indicates identity
Score > 25 indicates homology		 U K.VAQQGAYDAIIALGAVIR.G
2903   610.3533 1828.0381 1828.0207 9.53 0 47 0.001 +1Score > 33 indicates identity
Score > 30 indicates homology		 U K.VAQQGAYDAIIALGAVIR.G

4 subsets and intersections (7 subset proteins in total)

Score Mass Subset of
RISB_PSEU5 72 16536 36.1
6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas stutzeri (strain A1501) GN=ribH PE=3 SV=1
RISB_PSEMY 37 16541 36.1
6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas mendocina (strain ymp) GN=ribH PE=3 SV=1
RISB1_PSESM 34 16567 36.1
6,7-dimethyl-8-ribityllumazine synthase 1 OS=Pseudomonas syringae pv. tomato GN=ribH1 PE=3 SV=1
RISB_PSEA7 32 16460 36.1
6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas aeruginosa (strain PA7) GN=ribH PE=3 SV=1
3 samesets of RISB_PSEA7
RISB_PSEAB 32 16460
6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=ribH PE=3 SV=1
RISB_PSEAE 32 16460
6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas aeruginosa GN=ribH PE=3 SV=1
RISB_PSEA8 32 16460
6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas aeruginosa (strain LESB58) GN=ribH PE=3 SV=1

+37

Accession Score Description
1 CYOA_PSEPU 155 Ubiquinol oxidase subunit 2 OS=Pseudomonas putida GN=cyoA PE=3 SV=1

+38

Accession Score Description
1 AROG_ECOLI 155 Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive OS=Escherichia coli (strain K12) GN=aroG PE=1 SV=1

+39

Accession Score Description
1 RL28_PSEP1 150 50S ribosomal protein L28 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpmB PE=3 SV=1

+40

Accession Score Description
1 DBHB_PSEAE 148 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3
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