MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 230)

Page: Previous 1 2 3 4 5 6 7 8 9 … 23 Next 

+31

Accession Score Description
1 OXAA_PSEPK 175 Inner membrane protein oxaA OS=Pseudomonas putida (strain KT2440) GN=oxaA PE=3 SV=1

+32

Accession Score Description
Family member distances as a dendrogram 1 RS3_PSEE4 173 30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1
2 RS3_NITOC 80 30S ribosomal protein S3 OS=Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) GN=rpsC PE=3 SV=1

+33

Accession Score Description
1 ODO1_AZOVI 172 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

+34

Accession Score Description
1 RS11_PSEE4 169 30S ribosomal protein S11 OS=Pseudomonas entomophila (strain L48) GN=rpsK PE=3 SV=1

-35

Accession Score Description
Family member distances as a dendrogram 1 RS9_PSEE4 161 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1
2 RS9_PSE14 90 30S ribosomal protein S9 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsI PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
RS9_PSEE4 161 14597 12 (7) 5 (4) 1.46
30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1
3 samesets of RS9_PSEE4
RS9_PSEP1 161 14583 12 (7) 5 (4) 1.48
30S ribosomal protein S9 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsI PE=3 SV=1
RS9_PSEPG 161 14597 12 (7) 5 (4) 1.46
30S ribosomal protein S9 OS=Pseudomonas putida (strain GB-1) GN=rpsI PE=3 SV=1
RS9_PSEPK 161 14583 12 (7) 5 (4) 1.48
30S ribosomal protein S9 OS=Pseudomonas putida (strain KT2440) GN=rpsI PE=3 SV=1
RS9_PSE14 90 14593 6 (5) 3 (3) 0.83
30S ribosomal protein S9 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsI PE=3 SV=1
3 samesets of RS9_PSE14
RS9_PSEFS 90 14607 6 (5) 3 (3) 0.82
30S ribosomal protein S9 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsI PE=3 SV=1
RS9_PSESM 90 14593 6 (5) 3 (3) 0.83
30S ribosomal protein S9 OS=Pseudomonas syringae pv. tomato GN=rpsI PE=3 SV=1
RS9_PSEU2 90 14593 6 (5) 3 (3) 0.83
30S ribosomal protein S9 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsI PE=3 SV=1

-13 peptide matches (10 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
394   333.6828 665.3510 665.3497 2.07 0 32 0.11 +1Score > 42 indicates identity
Score > 35 indicates homology		 X R.AGYVTR.D
395   666.3594 665.3521 665.3497 3.69 0 23 1.1 +2Score > 39 indicates identity
Score > 36 indicates homology		 X R.AGYVTR.D
1004 +2 470.7502 939.4858 939.4814 4.70 0 50 0.0048 +1Score > 39 indicates identity U X R.SLDVFFGR.E
1006   940.4968 939.4895 939.4814 8.62 0 37 0.012 +1Score > 39 indicates identity
Score > 30 indicates homology		 U X R.SLDVFFGR.E
1254   527.7517 1053.4888 1053.4839 4.67 0 68 9.5e-005 +1Score > 40 indicates identity U X X M.SATQNYGTGR.R
1684   620.7993 1239.5840 1239.5805 2.86 1 24 0.025 +1Score > 40 indicates identity
Score > 21 indicates homology		 U X R.ALMQYDETLR.G + Deamidated (NQ)
2536 +1 553.3102 1656.9088 1656.9060 1.69 0 39 0.017 +1Score > 38 indicates identity
Score > 34 indicates homology		 U X X R.VFLRPGTGNISINNR.S
2537   829.4620 1656.9094 1656.9060 2.10 0 51 0.0015 +1Score > 38 indicates identity
Score > 35 indicates homology		 U X X R.VFLRPGTGNISINNR.S
2540   553.6454 1657.9144 1657.8900 14.7 0 22 0.086 +1Score > 37 indicates identity
Score > 24 indicates homology		 U X X R.VFLRPGTGNISINNR.S + Deamidated (NQ)
3082   977.5109 1953.0072 1952.9956 5.95 0 75 2.1e-007 +1Score > 39 indicates identity
Score > 20 indicates homology		 U X K.FDIYVTVSGGGVSGQAGAIR.H

3 subsets and intersections (13 subset proteins in total)

Score Mass Subset of
RS9_PSEF5 89 14610 35.1
30S ribosomal protein S9 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsI PE=3 SV=1
2 samesets of RS9_PSEF5
RS9_PSEMY 89 14637
30S ribosomal protein S9 OS=Pseudomonas mendocina (strain ymp) GN=rpsI PE=3 SV=1
RS9_PSEPF 89 14597
30S ribosomal protein S9 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsI PE=3 SV=1
RS9_AZOVD 68 14723 35.1
30S ribosomal protein S9 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsI PE=3 SV=1
6 samesets of RS9_AZOVD
RS9_PSEA7 68 14616
30S ribosomal protein S9 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsI PE=3 SV=1
RS9_PSEA8 68 14606
30S ribosomal protein S9 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsI PE=3 SV=1
RS9_PSEAB 68 14606
30S ribosomal protein S9 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsI PE=3 SV=1
RS9_PSEAE 68 14606
30S ribosomal protein S9 OS=Pseudomonas aeruginosa GN=rpsI PE=3 SV=1
RS9_PSEPW 68 14581
30S ribosomal protein S9 OS=Pseudomonas putida (strain W619) GN=rpsI PE=3 SV=1
RS9_PSEU5 68 14604
30S ribosomal protein S9 OS=Pseudomonas stutzeri (strain A1501) GN=rpsI PE=3 SV=1
RS9_AERHH 24 14776 35.2
30S ribosomal protein S9 OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) GN=rpsI PE=3 SV=1
2 samesets of RS9_AERHH
RS9_AERS4 24 14760
30S ribosomal protein S9 OS=Aeromonas salmonicida (strain A449) GN=rpsI PE=3 SV=1
RS9_MARMS 24 14733
30S ribosomal protein S9 OS=Marinomonas sp. (strain MWYL1) GN=rpsI PE=3 SV=1

+36

Accession Score Description
1 RISB_PSEPG 159 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

+37

Accession Score Description
1 CYOA_PSEPU 155 Ubiquinol oxidase subunit 2 OS=Pseudomonas putida GN=cyoA PE=3 SV=1

+38

Accession Score Description
1 AROG_ECOLI 155 Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive OS=Escherichia coli (strain K12) GN=aroG PE=1 SV=1

+39

Accession Score Description
1 RL28_PSEP1 150 50S ribosomal protein L28 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpmB PE=3 SV=1

+40

Accession Score Description
1 DBHB_PSEAE 148 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3
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