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Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 230)

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+31

Accession Score Description
1 OXAA_PSEPK 175 Inner membrane protein oxaA OS=Pseudomonas putida (strain KT2440) GN=oxaA PE=3 SV=1

+32

Accession Score Description
Family member distances as a dendrogram 1 RS3_PSEE4 173 30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1
2 RS3_NITOC 80 30S ribosomal protein S3 OS=Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) GN=rpsC PE=3 SV=1

+33

Accession Score Description
1 ODO1_AZOVI 172 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

-34

Accession Score Description
1 RS11_PSEE4 169 30S ribosomal protein S11 OS=Pseudomonas entomophila (strain L48) GN=rpsK PE=3 SV=1
Score Mass Matches Sequences emPAI
34.1 RS11_PSEE4 169 13765 8 (4) 5 (4) 0.89
30S ribosomal protein S11 OS=Pseudomonas entomophila (strain L48) GN=rpsK PE=3 SV=1
6 samesets of RS11_PSEE4
RS11_PSEMY 169 13753 8 (4) 5 (4) 0.89
30S ribosomal protein S11 OS=Pseudomonas mendocina (strain ymp) GN=rpsK PE=3 SV=1
RS11_PSEP1 169 13765 8 (4) 5 (4) 0.89
30S ribosomal protein S11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsK PE=3 SV=1
RS11_PSEPG 169 13779 8 (4) 5 (4) 0.89
30S ribosomal protein S11 OS=Pseudomonas putida (strain GB-1) GN=rpsK PE=3 SV=1
RS11_PSEPK 169 13765 8 (4) 5 (4) 0.89
30S ribosomal protein S11 OS=Pseudomonas putida (strain KT2440) GN=rpsK PE=3 SV=1
RS11_PSEPW 169 13765 8 (4) 5 (4) 0.89
30S ribosomal protein S11 OS=Pseudomonas putida (strain W619) GN=rpsK PE=3 SV=1
RS11_PSEU5 169 13749 8 (4) 5 (4) 0.89
30S ribosomal protein S11 OS=Pseudomonas stutzeri (strain A1501) GN=rpsK PE=3 SV=1

-8 peptide matches (6 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
683   401.2282 800.4418 800.4392 3.30 0 10 1.1 +2Score > 43 indicates identity
Score > 23 indicates homology		 U K.NLDVNVK.G
1390 +1 560.8047 1119.5948 1119.5924 2.19 1 66 0.00015 +1Score > 41 indicates identity U R.AGQAALEYGLK.N
1710   631.3314 1260.6482 1260.6462 1.60 1 67 8.8e-006 +1Score > 42 indicates identity
Score > 29 indicates homology		 U K.STPFAAQIAAER.A
2383   798.3823 1594.7500 1594.7488 0.77 0 90 5.7e-007 +1Score > 40 indicates identity U R.QGNALSWATSGGSGFR.G
3918   624.5867 2494.3177 2494.2816 14.5 0 28 0.18 +1Score > 36 indicates identity
Score > 33 indicates homology		 U K.TVVDGIAHIHASFNNTIVTITDR.Q + Deamidated (NQ)
3921 +1 832.4497 2494.3273 2494.2816 18.3 0 54 0.00074 +1Score > 36 indicates identity U K.TVVDGIAHIHASFNNTIVTITDR.Q + Deamidated (NQ)

6 subsets and intersections (51 subset proteins in total)

Score Mass Subset of
RS11_AZOVD 133 13735 34.1
30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1
10 samesets of RS11_AZOVD
RS11_PSE14 133 13721
30S ribosomal protein S11 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsK PE=3 SV=1
RS11_PSEA7 133 13735
30S ribosomal protein S11 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsK PE=3 SV=1
RS11_PSEA8 133 13735
30S ribosomal protein S11 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsK PE=3 SV=1
RS11_PSEAB 133 13735
30S ribosomal protein S11 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsK PE=3 SV=1
RS11_PSEAE 133 13735
30S ribosomal protein S11 OS=Pseudomonas aeruginosa GN=rpsK PE=3 SV=1
RS11_PSEF5 133 13735
30S ribosomal protein S11 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsK PE=3 SV=1
RS11_PSEFS 133 13721
30S ribosomal protein S11 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsK PE=3 SV=1
RS11_PSEPF 133 13721
30S ribosomal protein S11 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsK PE=3 SV=1
RS11_PSESM 133 13721
30S ribosomal protein S11 OS=Pseudomonas syringae pv. tomato GN=rpsK PE=3 SV=1
RS11_PSEU2 133 13721
30S ribosomal protein S11 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsK PE=3 SV=1
RS11_BLOFL 96 14138 34.1
30S ribosomal protein S11 OS=Blochmannia floridanus GN=rpsK PE=3 SV=1
RS11_AERHH 90 13883 34.1
30S ribosomal protein S11 OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) GN=rpsK PE=3 SV=1
+31 samesets of RS11_AERHH
RS11_BLOPB 67 14096 34.1
30S ribosomal protein S11 OS=Blochmannia pennsylvanicus (strain BPEN) GN=rpsK PE=3 SV=1
3 samesets of RS11_BLOPB
RS11_PSYIN 67 13857
30S ribosomal protein S11 OS=Psychromonas ingrahamii (strain 37) GN=rpsK1 PE=3 SV=1
RS11_BUCAP 67 14201
30S ribosomal protein S11 OS=Buchnera aphidicola subsp. Schizaphis graminum GN=rpsK PE=3 SV=1
RS11_DICNV 67 13973
30S ribosomal protein S11 OS=Dichelobacter nodosus (strain VCS1703A) GN=rpsK PE=3 SV=1
RS11_WIGBR 63 0 34.1
description
RS11_SACD2 54 13955 34.1
30S ribosomal protein S11 OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpsK PE=3 SV=1
1 sameset of RS11_SACD2
RS11_TERTT 54 13913
30S ribosomal protein S11 OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=rpsK PE=3 SV=1

+35

Accession Score Description
Family member distances as a dendrogram 1 RS9_PSEE4 161 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1
2 RS9_PSE14 90 30S ribosomal protein S9 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsI PE=3 SV=1

+36

Accession Score Description
1 RISB_PSEPG 159 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

+37

Accession Score Description
1 CYOA_PSEPU 155 Ubiquinol oxidase subunit 2 OS=Pseudomonas putida GN=cyoA PE=3 SV=1

+38

Accession Score Description
1 AROG_ECOLI 155 Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive OS=Escherichia coli (strain K12) GN=aroG PE=1 SV=1

+39

Accession Score Description
1 RL28_PSEP1 150 50S ribosomal protein L28 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpmB PE=3 SV=1

+40

Accession Score Description
1 DBHB_PSEAE 148 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3
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