MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 230)

Page: Previous 1 2 3 4 5 6 7 8 9 … 23 Next 

+31

Accession Score Description
1 OXAA_PSEPK 175 Inner membrane protein oxaA OS=Pseudomonas putida (strain KT2440) GN=oxaA PE=3 SV=1

-32

Accession Score Description
Family member distances as a dendrogram 1 RS3_PSEE4 173 30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1
2 RS3_NITOC 80 30S ribosomal protein S3 OS=Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) GN=rpsC PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
RS3_PSEE4 173 25709 28 (8) 11 (5) 0.83
30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1
3 samesets of RS3_PSEE4
RS3_PSEP1 173 25709 28 (8) 11 (5) 0.83
30S ribosomal protein S3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsC PE=3 SV=1
RS3_PSEPK 173 25709 28 (8) 11 (5) 0.83
30S ribosomal protein S3 OS=Pseudomonas putida (strain KT2440) GN=rpsC PE=3 SV=2
RS3_PSEU5 173 25730 28 (8) 11 (5) 0.83
30S ribosomal protein S3 OS=Pseudomonas stutzeri (strain A1501) GN=rpsC PE=3 SV=1
RS3_NITOC 80 24932 11 (4) 5 (3) 0.43
30S ribosomal protein S3 OS=Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) GN=rpsC PE=3 SV=1

-29 peptide matches (23 non-duplicate, 6 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
239   529.3723 528.3650 528.3635 2.84 0 18 0.57 +1Score > 43 indicates identity
Score > 28 indicates homology		 X X R.LGIVK  
384   330.1959 658.3772 658.3762 1.56 0 30 0.35 +1Score > 44 indicates identity
Score > 38 indicates homology		 U X K.IQVSGR.L
386   659.3857 658.3784 658.3762 3.35 0 36 0.056 +1Score > 42 indicates identity
Score > 36 indicates homology		 U X K.IQVSGR.L
454   692.4158 691.4085 691.4057 4.04 0 19 0.81 +1Score > 37 indicates identity
Score > 31 indicates homology		 X X K.VWIFK.G
456 +1 346.7126 691.4106 691.4057 7.11 0 20 0.9 +1Score > 37 indicates identity
Score > 32 indicates homology		 X X K.VWIFK.G
579   377.6804 753.3462 753.3446 2.22 1 31 0.14 +1Score > 39 indicates identity
Score > 35 indicates homology		 X R.TEWYR.E
580   754.3537 753.3464 753.3446 2.46 1 21 1.4 +2Score > 39 indicates identity
Score > 35 indicates homology		 X R.TEWYR.E
646   786.4484 785.4411 785.4395 2.03 1 34 0.4 +1Score > 43 indicates identity X R.LGGAEIAR.T
647 +1 393.7281 785.4416 785.4395 2.70 1 32 0.27 +1Score > 43 indicates identity
Score > 39 indicates homology		 X R.LGGAEIAR.T
652   789.4056 788.3983 788.3963 2.58 0 24 1.4 +2Score > 44 indicates identity
Score > 38 indicates homology		 X R.AVQNAMR.I
653   395.2068 788.3990 788.3963 3.50 0 16 1.2 +2Score > 44 indicates identity
Score > 30 indicates homology		 X R.AVQNAMR.I
665   795.3896 794.3823 794.3810 1.65 1 45 0.028 +1Score > 42 indicates identity U X R.EYLQDK.L
666   398.1987 794.3828 794.3810 2.31 1 23 0.95 +1Score > 41 indicates identity
Score > 35 indicates homology		 U X R.EYLQDK.L
752   418.2626 834.5106 834.5076 3.69 0 33 0.053 +1Score > 33 indicates identity
Score > 33 indicates homology		 U X X R.VPLHTLR.A
1738 +1 320.1830 1276.7029 1276.7000 2.27 0 23 0.078 +1Score > 39 indicates identity
Score > 25 indicates homology		 U X R.IDIHRPAQTAR.I
1741   426.5754 1276.7044 1276.7000 3.43 0 18 0.25 +1Score > 38 indicates identity
Score > 24 indicates homology		 U X R.IDIHRPAQTAR.I
1742   639.3599 1276.7052 1276.7000 4.11 0 26 0.3 +1Score > 38 indicates identity
Score > 33 indicates homology		 U X R.IDIHRPAQTAR.I
2127 +2 369.7334 1474.9045 1474.8984 4.15 0 9 1 +1Score > 26 indicates identity
Score > 22 indicates homology		 U X X R.ITIHTARPGIVIGK.K
2128   738.4596 1474.9046 1474.8984 4.25 0 31 0.02 +1Score > 26 indicates identity U X X R.ITIHTARPGIVIGK.K
2129   492.6426 1474.9060 1474.8984 5.15 0 22 0.037 +1Score > 25 indicates identity
Score > 20 indicates homology		 U X X R.ITIHTARPGIVIGK.K
2525   551.2980 1650.8722 1650.8585 8.26 1 80 2.2e-006 +1Score > 39 indicates identity
Score > 36 indicates homology		 U X R.MMGIPVHINIQEIR.K + Deamidated (NQ)
3583   1116.0438 2230.0730 2230.0430 13.5 1 97 1.4e-009 +1Score > 39 indicates identity
Score > 20 indicates homology		 U X R.ADIDYNTYEAHTTYGVIGVK.V + Deamidated (NQ)
3584 +1 744.3653 2230.0741 2230.0430 13.9 1 61 9.7e-006 +1Score > 39 indicates identity
Score > 24 indicates homology		 U X R.ADIDYNTYEAHTTYGVIGVK.V + Deamidated (NQ)

8 subsets and intersections (477 subset proteins in total)

Score Mass Subset of
CR019_BOVIN 45 31176 32.1
Uncharacterized protein C18orf19 homolog OS=Bos taurus PE=2 SV=1
1 sameset of CR019_BOVIN
CSK2C_DROME 45 25349
Casein kinase II subunit beta' OS=Drosophila melanogaster GN=CkIIbeta2 PE=1 SV=1
RS3_PSE14 41 25737 32.1
30S ribosomal protein S3 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsC PE=3 SV=1
7 samesets of RS3_PSE14
RS3_PSEA7 41 25822
30S ribosomal protein S3 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsC PE=3 SV=1
RS3_PSEAB 41 25822
30S ribosomal protein S3 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsC PE=3 SV=1
RS3_PSEAE 41 25822
30S ribosomal protein S3 OS=Pseudomonas aeruginosa GN=rpsC PE=3 SV=1
RS3_PSEF5 41 25676
30S ribosomal protein S3 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsC PE=3 SV=1
RS3_PSEPF 41 25737
30S ribosomal protein S3 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsC PE=3 SV=1
RS3_PSESM 41 25737
30S ribosomal protein S3 OS=Pseudomonas syringae pv. tomato GN=rpsC PE=3 SV=1
RS3_PSEU2 41 25737
30S ribosomal protein S3 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsC PE=3 SV=1
RR3_CYACA 36 23417 32.1
30S ribosomal protein S3, chloroplastic OS=Cyanidium caldarium GN=rps3 PE=3 SV=1
13 samesets of RR3_CYACA
RR3_GRATL 36 25097
30S ribosomal protein S3, chloroplastic OS=Gracilaria tenuistipitata var. liui GN=rps3 PE=3 SV=1
RR3_ODOSI 36 23967
30S ribosomal protein S3, chloroplastic OS=Odontella sinensis GN=rps3 PE=3 SV=1
RR3_PHATR 36 24098
30S ribosomal protein S3, chloroplastic OS=Phaeodactylum tricornutum GN=rps3 PE=3 SV=1
RR3_PORPU 36 25673
30S ribosomal protein S3, chloroplastic OS=Porphyra purpurea GN=rps3 PE=3 SV=1
RR3_PORYE 36 25742
30S ribosomal protein S3, chloroplastic OS=Porphyra yezoensis GN=rps3 PE=3 SV=1
RR3_GRATE 36 24125
30S ribosomal protein S3, chloroplastic OS=Gracilaria tenuistipitata GN=rps3 PE=3 SV=1
RS3_TRIEI 36 27334
30S ribosomal protein S3 OS=Trichodesmium erythraeum (strain IMS101) GN=rpsC PE=3 SV=1
RR3_CYAME 36 23326
30S ribosomal protein S3, chloroplastic OS=Cyanidioschyzon merolae GN=rps3 PE=3 SV=1
RR3_EMIHU 36 24586
30S ribosomal protein S3, chloroplastic OS=Emiliania huxleyi GN=rps3 PE=3 SV=1
RR3_OLTVI 36 27193
30S ribosomal protein S3, chloroplastic OS=Oltmannsiellopsis viridis GN=rps3 PE=3 SV=1
RR3_SCEOB 36 81957
30S ribosomal protein S3, chloroplastic OS=Scenedesmus obliquus GN=rps3 PE=3 SV=1
RS3_PORG3 36 27667
30S ribosomal protein S3 OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / JCM 12257) GN=rpsC PE=3 SV=1
RS3_PORGI 36 27667
30S ribosomal protein S3 OS=Porphyromonas gingivalis GN=rpsC PE=3 SV=1
RS3_PSEMY 36 25525 32.1
30S ribosomal protein S3 OS=Pseudomonas mendocina (strain ymp) GN=rpsC PE=3 SV=1
RS3_CHLAB 35 24611 32.1
30S ribosomal protein S3 OS=Chlamydophila abortus GN=rpsC PE=3 SV=1
16 samesets of RS3_CHLAB
RS3_CHLPN 35 24740
30S ribosomal protein S3 OS=Chlamydia pneumoniae GN=rpsC PE=3 SV=2
RS3_CHLCV 35 24718
30S ribosomal protein S3 OS=Chlamydophila caviae GN=rpsC PE=3 SV=1
RS3_CHLFF 35 24681
30S ribosomal protein S3 OS=Chlamydophila felis (strain Fe/C-56) GN=rpsC PE=3 SV=1
RS3_MICAN 35 27473
30S ribosomal protein S3 OS=Microcystis aeruginosa (strain NIES-843) GN=rpsC PE=3 SV=1
RS3_SYNP2 35 27171
30S ribosomal protein S3 OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) GN=rpsC PE=3 SV=1
RS3_SYNY3 35 27188
30S ribosomal protein S3 OS=Synechocystis sp. (strain PCC 6803) GN=rpsC PE=1 SV=3
RS3_CLAM3 35 29536
30S ribosomal protein S3 OS=Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382) GN=rpsC PE=3 SV=1
RS3_CLAMS 35 29536
30S ribosomal protein S3 OS=Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / JCM 9667) GN=rpsC PE=3 SV=1
RS3_SYNPX 35 27090
30S ribosomal protein S3 OS=Synechococcus sp. (strain WH8102) GN=rpsC PE=3 SV=1
RS3_SYNSC 35 27122
30S ribosomal protein S3 OS=Synechococcus sp. (strain CC9605) GN=rpsC PE=3 SV=1
RS3_NOSP7 35 30087
30S ribosomal protein S3 OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) GN=rpsC PE=3 SV=1
RS3_LEIXX 35 28469
30S ribosomal protein S3 OS=Leifsonia xyli subsp. xyli GN=rpsC PE=3 SV=1
RS3_CYAP8 35 27627
30S ribosomal protein S3 OS=Cyanothece sp. (strain PCC 8801) GN=rpsC PE=3 SV=1
RS3_SYNPW 35 27283
30S ribosomal protein S3 OS=Synechococcus sp. (strain WH7803) GN=rpsC PE=3 SV=1
RS3_SYNS3 35 27261
30S ribosomal protein S3 OS=Synechococcus sp. (strain CC9311) GN=rpsC PE=3 SV=1
RS3_SYNS9 35 27262
30S ribosomal protein S3 OS=Synechococcus sp. (strain CC9902) GN=rpsC PE=3 SV=1
RR3_AMBTC 33 25353 32.1
30S ribosomal protein S3, chloroplastic OS=Amborella trichopoda GN=rps3 PE=3 SV=1
+425 samesets of RR3_AMBTC
RS3_CHRSD 33 26097 32.1
30S ribosomal protein S3 OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpsC PE=3 SV=1
2 samesets of RS3_CHRSD
RS3_HAHCH 33 25467
30S ribosomal protein S3 OS=Hahella chejuensis (strain KCTC 2396) GN=rpsC PE=3 SV=1
RS3_MARAV 33 25498
30S ribosomal protein S3 OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=rpsC PE=3 SV=1
RS3_FRATF 30 24862 32.1
30S ribosomal protein S3 OS=Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA) GN=rpsC PE=3 SV=1
5 samesets of RS3_FRATF
RS3_FRATH 30 24862
30S ribosomal protein S3 OS=Francisella tularensis subsp. holarctica (strain LVS) GN=rpsC PE=3 SV=1
RS3_FRATM 30 24848
30S ribosomal protein S3 OS=Francisella tularensis subsp. mediasiatica (strain FSC147) GN=rpsC PE=3 SV=1
RS3_FRATN 30 24792
30S ribosomal protein S3 OS=Francisella tularensis subsp. novicida (strain U112) GN=rpsC PE=3 SV=1
RS3_FRATO 30 24862
30S ribosomal protein S3 OS=Francisella tularensis subsp. holarctica (strain OSU18) GN=rpsC PE=3 SV=1
RS3_FRATW 30 24862
30S ribosomal protein S3 OS=Francisella tularensis subsp. tularensis (strain WY96-3418) GN=rpsC PE=3 SV=1

+33

Accession Score Description
1 ODO1_AZOVI 172 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

+34

Accession Score Description
1 RS11_PSEE4 169 30S ribosomal protein S11 OS=Pseudomonas entomophila (strain L48) GN=rpsK PE=3 SV=1

+35

Accession Score Description
Family member distances as a dendrogram 1 RS9_PSEE4 161 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1
2 RS9_PSE14 90 30S ribosomal protein S9 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsI PE=3 SV=1

+36

Accession Score Description
1 RISB_PSEPG 159 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

+37

Accession Score Description
1 CYOA_PSEPU 155 Ubiquinol oxidase subunit 2 OS=Pseudomonas putida GN=cyoA PE=3 SV=1

+38

Accession Score Description
1 AROG_ECOLI 155 Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive OS=Escherichia coli (strain K12) GN=aroG PE=1 SV=1

+39

Accession Score Description
1 RL28_PSEP1 150 50S ribosomal protein L28 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpmB PE=3 SV=1

+40

Accession Score Description
1 DBHB_PSEAE 148 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3
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