MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 230)

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+21

Accession Score Description
1 ARPB_PSEPU 217 Antibiotic efflux pump membrane transporter arpB OS=Pseudomonas putida GN=arpB PE=2 SV=1

+22

Accession Score Description
Family member distances as a dendrogram 1 RL5_PSEE4 215 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
2 RL5_FLAPJ 47 50S ribosomal protein L5 OS=Flavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) GN=rplE PE=3 SV=1

+23

Accession Score Description
1 RL11_PSEP1 215 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1

+24

Accession Score Description
1 RPOA_PSEPK 211 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+25

Accession Score Description
1 RL1_PSEE4 210 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+26

Accession Score Description
1 RS20_PSE14 201 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

+27

Accession Score Description
1 ATPG_PSEPG 200 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

+28

Accession Score Description
1 ATPF_PSEPG 193 ATP synthase subunit b OS=Pseudomonas putida (strain GB-1) GN=atpF PE=3 SV=1

+29

Accession Score Description
1 RL16_PSEP1 184 50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1

-30

Accession Score Description
1 RS16_PSEP1 179 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1
Score Mass Matches Sequences emPAI
30.1 RS16_PSEP1 179 9183 11 (7) 5 (4) 2.24
30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1
2 samesets of RS16_PSEP1
RS16_PSEPG 179 9183 11 (7) 5 (4) 2.24
30S ribosomal protein S16 OS=Pseudomonas putida (strain GB-1) GN=rpsP PE=3 SV=1
RS16_PSEPK 179 9183 11 (7) 5 (4) 2.24
30S ribosomal protein S16 OS=Pseudomonas putida (strain KT2440) GN=rpsP PE=3 SV=1

-11 peptide matches (8 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
406   336.2270 670.4394 670.4377 2.54 0 10 1.1 +4Score > 37 indicates identity
Score > 23 indicates homology		 U R.VAQLLK.E
408   671.4483 670.4410 670.4377 4.89 0 41 0.02 +2Score > 37 indicates identity U R.VAQLLK.E
773 +1 423.2283 844.4420 844.4403 2.12 1 53 0.0056 +1Score > 43 indicates identity U K.LSVNQER.V
774   845.4495 844.4422 844.4403 2.32 1 35 0.15 +1Score > 43 indicates identity
Score > 39 indicates homology		 U K.LSVNQER.V
2018 +2 710.3951 1418.7756 1418.7558 14.0 1 53 0.00017 +1Score > 39 indicates identity
Score > 28 indicates homology		 U R.VGFFNPIAAGAEVK.L
2450   405.7280 1618.8829 1618.8580 15.4 1 7 0.94 +1Score > 37 indicates identity
Score > 20 indicates homology		 U K.KRPFYHLTVTNSR.N + Deamidated (NQ)
2452   811.4037 1620.7928 1620.7896 2.00 1 81 5.2e-006 +1Score > 41 indicates identity U R.VTYWLSQGAQPSER.V
2453   541.2729 1620.7969 1620.7896 4.48 1 63 0.00034 +1Score > 41 indicates identity U R.VTYWLSQGAQPSER.V

5 subsets and intersections (31 subset proteins in total)

Score Mass Subset of
RS16_PSEE4 117 9199 30.1
30S ribosomal protein S16 OS=Pseudomonas entomophila (strain L48) GN=rpsP PE=3 SV=1
RS16_PSEPW 114 9155 30.1
30S ribosomal protein S16 OS=Pseudomonas putida (strain W619) GN=rpsP PE=3 SV=1
RS16_PSEF5 54 9230 30.1
30S ribosomal protein S16 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsP PE=3 SV=1
2 samesets of RS16_PSEF5
RS16_PSEPF 54 9230
30S ribosomal protein S16 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsP PE=3 SV=1
RS16_PSEU5 54 9195
30S ribosomal protein S16 OS=Pseudomonas stutzeri (strain A1501) GN=rpsP PE=3 SV=1
RS16_PSEFS 53 9202 30.1
30S ribosomal protein S16 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsP PE=3 SV=1
ATPO_CALJA 41 0 30.1
description
+24 samesets of ATPO_CALJA
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