MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 230)

Page: 1 2 3 4 5 6 … 23 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 1648 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_LEGPA 425 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1599 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
5 EFTU_MYCS5 42 Elongation factor Tu OS=Mycoplasma synoviae (strain 53) GN=tuf PE=3 SV=1
3 EFTU_CARRP 146 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
4 EFTU_ANATD 54 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1484 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

-3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 907 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
3 ATPA_RICAH 176 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
2 ATPA_VEREI 178 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
ATPA_PSEPK 907 55489 59 (41) 26 (18) 1.64
ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
1 sameset of ATPA_PSEPK
ATPA_PSEP1 907 55458 59 (41) 26 (18) 1.64
ATP synthase subunit alpha OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpA PE=3 SV=1
ATPA_VEREI 178 57757 12 (8) 5 (4) 0.26
ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1
ATPA_RICAH 176 56389 13 (11) 5 (5) 0.32
ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
7 samesets of ATPA_RICAH
ATPA_RICCN 176 56128 13 (11) 5 (5) 0.32
ATP synthase subunit alpha OS=Rickettsia conorii GN=atpA PE=3 SV=2
ATPA_RICFE 176 56172 13 (11) 5 (5) 0.32
ATP synthase subunit alpha OS=Rickettsia felis GN=atpA PE=3 SV=1
ATPA_RICPU 176 56160 13 (11) 5 (5) 0.32
ATP synthase subunit alpha OS=Rickettsia peacockii (strain Rustic) GN=atpA PE=3 SV=1
ATPA_RICRO 176 56195 13 (11) 5 (5) 0.32
ATP synthase subunit alpha OS=Rickettsia rickettsii (strain Iowa) GN=atpA PE=3 SV=2
ATPA_RICRS 176 56195 13 (11) 5 (5) 0.32
ATP synthase subunit alpha OS=Rickettsia rickettsii (strain Sheila Smith) GN=atpA PE=3 SV=1
ATPA_RICM5 176 56127 13 (11) 5 (5) 0.32
ATP synthase subunit alpha OS=Rickettsia massiliae (strain Mtu5) GN=atpA PE=3 SV=2
ATPA_RICB8 176 56616 13 (11) 5 (5) 0.32
ATP synthase subunit alpha OS=Rickettsia bellii (strain OSU 85-389) GN=atpA PE=3 SV=1

-64 peptide matches (43 non-duplicate, 21 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
301   301.6854 601.3562 601.3548 2.49 0 22 1.4 +4Score > 43 indicates identity
Score > 35 indicates homology		 X K.LSGGIR.T
302   602.3646 601.3573 601.3548 4.28 0 22 1 +3Score > 43 indicates identity
Score > 34 indicates homology		 X K.LSGGIR.T
310   606.2899 605.2826 605.2809 2.80 0 10 0.83 +1Score > 40 indicates identity
Score > 22 indicates homology		 U X K.ATQTW.-
353   318.1801 634.3456 634.3438 2.85 0 32 0.046 +1Score > 41 indicates identity
Score > 31 indicates homology		 U X E.LAQFR.E + Deamidated (NQ)
489   354.1963 706.3780 706.3762 2.61 0 32 0.14 +1Score > 41 indicates identity
Score > 36 indicates homology		 X X K.QAVAYR.Q
490   707.3864 706.3791 706.3762 4.13 0 25 0.75 +1Score > 40 indicates identity
Score > 36 indicates homology		 X X K.QAVAYR.Q
509   360.7031 719.3916 719.3887 4.03 1 26 0.17 +1Score > 41 indicates identity
Score > 30 indicates homology		 X R.VTELMK.Q
707   408.2351 814.4556 814.4548 0.98 1 22 1.3 +4Score > 43 indicates identity
Score > 36 indicates homology		 U X X X R.ELIIGDR.Q
708   815.4647 814.4574 814.4548 3.16 1 45 0.036 +1Score > 43 indicates identity U X X X R.ELIIGDR.Q
717   822.4489 821.4416 821.4395 2.55 0 36 0.16 +1Score > 40 indicates identity U X R.TALAQYR.E
718   411.7283 821.4420 821.4395 3.07 0 39 0.078 +1Score > 40 indicates identity U X R.TALAQYR.E
767   842.5493 841.5420 841.5385 4.17 0 43 0.014 +1Score > 37 indicates identity U X X R.QISLLLR.R
770 +2 421.7799 841.5452 841.5385 8.00 0 41 0.013 +1Score > 35 indicates identity U X X R.QISLLLR.R
806 +2 427.7725 853.5304 853.5273 3.69 1 43 0.0055 +1Score > 33 indicates identity U X R.ILEVPVGK.E
808   854.5380 853.5307 853.5273 4.01 1 45 0.0029 +1Score > 33 indicates identity U X R.ILEVPVGK.E
824   859.5016 858.4943 858.4923 2.35 0 39 0.11 +1Score > 42 indicates identity U X R.STVANIVR.K
825 +1 430.2549 858.4952 858.4923 3.42 0 39 0.093 +1Score > 42 indicates identity U X R.STVANIVR.K
898   447.7406 893.4666 893.4607 6.67 0 36 0.042 +1Score > 41 indicates identity
Score > 35 indicates homology		 U X K.FTNGAVTGK.T
899   448.2292 894.4438 894.4447 -0.95 0 39 0.041 +1Score > 41 indicates identity
Score > 37 indicates homology		 U X K.FTNGAVTGK.T + Deamidated (NQ)
900   897.5327 896.5254 896.5232 2.45 0 21 0.53 +1Score > 36 indicates identity
Score > 30 indicates homology		 U X K.VAPGVIWR.K
902 +2 449.2711 896.5276 896.5232 4.93 0 46 0.0049 +1Score > 36 indicates identity U X K.VAPGVIWR.K
940   457.7245 913.4344 913.4327 1.89 1 24 0.15 +1Score > 39 indicates identity
Score > 29 indicates homology		 U X R.DHAELMAK.I
1241   522.7797 1043.5448 1043.5434 1.42 0 37 0.063 +1Score > 42 indicates identity
Score > 37 indicates homology		 U X K.SVDAMIPVGR.G
1365 +3 553.8141 1105.6136 1105.6019 10.6 1 43 0.0019 +1Score > 39 indicates identity
Score > 28 indicates homology		 U X R.GFLIDVEVSK.I
1581 +1 599.3290 1196.6434 1196.6401 2.79 0 64 0.00017 +1Score > 39 indicates identity U X X R.VVDALGNPIDGK.G
1623 +1 609.3114 1216.6082 1216.6048 2.86 0 70 7.8e-005 +1Score > 42 indicates identity U X R.IDNLDVSSQAR.N
1764 +1 644.8574 1287.7002 1287.6856 11.3 0 53 0.0029 +1Score > 40 indicates identity U X K.TAMAIDAIINQK.D
1771 +1 647.3045 1292.5944 1292.5885 4.64 1 95 5.5e-008 +1Score > 40 indicates identity
Score > 35 indicates homology		 U X K.GDFNDEIDAGLK.A
1837   665.3377 1328.6608 1328.6572 2.74 1 94 3.9e-008 +1Score > 41 indicates identity
Score > 32 indicates homology		 U X K.GPLGNTQTDAVEK.V
1865   675.3582 1348.7018 1348.6987 2.35 1 95 9.1e-008 +1Score > 40 indicates identity
Score > 37 indicates homology		 U X R.KSVDQPVQTGYK.S
1866   450.5753 1348.7041 1348.6987 3.99 1 33 0.0092 +1Score > 40 indicates identity
Score > 25 indicates homology		 U X R.KSVDQPVQTGYK.S
2048   477.5852 1429.7338 1429.7273 4.50 1 24 0.34 +1Score > 41 indicates identity
Score > 32 indicates homology		 U X K.GRIDNLDVSSQAR.N
2049   715.8743 1429.7340 1429.7273 4.69 1 74 7e-006 +1Score > 41 indicates identity
Score > 35 indicates homology		 U X K.GRIDNLDVSSQAR.N
2050 +1 716.3784 1430.7422 1430.7365 3.99 1 78 3e-006 +1Score > 41 indicates identity
Score > 36 indicates homology		 U X R.NEGTVVSVSDGIVR.I
2058   719.3671 1436.7196 1436.7035 11.3 0 50 0.0071 +1Score > 41 indicates identity U X R.GQDALIVYDDLSK.Q + Deamidated (NQ)
2058   719.3671 1436.7196 1436.7035 11.3 1 48 0.011 +3Score > 41 indicates identity U X R.GEDALIVYDDLSK.Q
2291 +2 518.5873 1552.7401 1552.7310 5.82 1 52 0.0017 +1Score > 41 indicates identity
Score > 37 indicates homology		 U X X X R.EAYPGDVFYLHSR.L
2292   777.3774 1552.7402 1552.7310 5.93 1 86 1.5e-006 +1Score > 41 indicates identity U X X X R.EAYPGDVFYLHSR.L
2425 +1 806.9344 1611.8542 1611.8409 8.27 1 98 1e-008 +1Score > 39 indicates identity
Score > 30 indicates homology		 U X K.IGSFEQALIAFFNR.D
2426 +2 538.2935 1611.8587 1611.8409 11.0 1 78 6.9e-006 +1Score > 39 indicates identity U X K.IGSFEQALIAFFNR.D
2645   854.9288 1707.8430 1707.8315 6.75 1 82 3.4e-006 +1Score > 41 indicates identity
Score > 40 indicates homology		 U X R.DRGQDALIVYDDLSK.Q + Deamidated (NQ)
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_1_20250714114344.raw

Score > 41 indicates identity

Score > 33 indicates homology
2646 +1 570.2884 1707.8434 1707.8315 6.94 1 47 0.0023 -1Score > 41 indicates identity
Score > 33 indicates homology		 U X R.DRGQDALIVYDDLSK.Q + Deamidated (NQ)
12.7 0 18 2 2 GDFHSPVVLGRPPNSE   + Deamidated (NQ)
6.95 0 12 8.3 3 VSLNSAAIHNPNLIDE   + 2 Deamidated (NQ)
-12.9 1 11 9.9 4 ALRDMGIIDFGEPFK  
19.3 0 10 13 5 DFYNHAANILSDLSK   + Deamidated (NQ)
19.3 0 10 13 5 DFYNHAANILSDLSK   + Deamidated (NQ)
-9.75 1 10 13 7 LTMNQKSIVVNISNK   + 4 Deamidated (NQ); Oxidation (M)
2.61 0 8 17 8 TSFQMLPISNEPISK   + Deamidated (NQ); Oxidation (M)
2.61 0 8 19 9 TSFQMLPISNEPISK   + Deamidated (NQ); Oxidation (M)
4.59 1 8 20 10 IHDYDLLYSQIEAK   + Deamidated (NQ)
3339   701.3486 2101.0240 2100.9859 18.1 1 35 0.0044 +1Score > 40 indicates identity
Score > 24 indicates homology		 U X K.QYAPMSIADMALSLYAAER.G + Deamidated (NQ)

+63 subsets and intersections (1005 subset proteins in total)

+4

Accession Score Description
Family member distances as a dendrogram 1 PORF_PSESY 690 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1
2 PORF_PSEAE 143 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+5

Accession Score Description
Family member distances as a dendrogram 1 RPOB_PSEPG 513 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
5 FTSZ_PSEPK 129 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOC_PSEP1 418 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoC PE=3 SV=1
3 RPOC_PSEU5 335 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas stutzeri (strain A1501) GN=rpoC PE=3 SV=1
6 RPOC_BIFLD 91 DNA-directed RNA polymerase subunit beta' OS=Bifidobacterium longum (strain DJO10A) GN=rpoC PE=3 SV=1
4 RPOC_SYNAS 202 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1

+6

Accession Score Description
1 RS5_PSEE4 428 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+7

Accession Score Description
1 RL3_PSEP1 378 50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1

+8

Accession Score Description
1 ARPC_PSEPU 369 Antibiotic efflux pump outer membrane protein arpC OS=Pseudomonas putida GN=arpC PE=2 SV=1

+9

Accession Score Description
1 RL2_PSEP1 329 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1

+10

Accession Score Description
Family member distances as a dendrogram 1 DLDH2_PSEPU 325 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4
2 DLDH_AZOVI 161 Dihydrolipoyl dehydrogenase OS=Azotobacter vinelandii PE=1 SV=1
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