MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 sp |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 22:50:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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21| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARPB_PSEPU | 217 | Antibiotic efflux pump membrane transporter arpB OS=Pseudomonas putida GN=arpB PE=2 SV=1 |
22| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL5_PSEE4 | 215 | 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1 | |
| 2 | RL5_FLAPJ | 47 | 50S ribosomal protein L5 OS=Flavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) GN=rplE PE=3 SV=1 | |
23| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL11_PSEP1 | 215 | 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1 |
24| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOA_PSEPK | 211 | DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1 |
25| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL1_PSEE4 | 210 | 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1 |
26| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS20_PSE14 | 201 | 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1 |
27| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPG_PSEPG | 200 | ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1 |
28| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPF_PSEPG | 193 | ATP synthase subunit b OS=Pseudomonas putida (strain GB-1) GN=atpF PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 28.1 | ATPF_PSEPG |
193 | 16945 | 8 (7) | 4 (3) | 0.92 | |
| ATP synthase subunit b OS=Pseudomonas putida (strain GB-1) GN=atpF PE=3 SV=1 | |||||||
| 4 samesets of ATPF_PSEPG | |||||||
| ATPF_PSEPK |
193 | 16945 | 8 (7) | 4 (3) | 0.92 | ||
| ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1 | |||||||
| ATPF_PSEPW |
193 | 16945 | 8 (7) | 4 (3) | 0.92 | ||
| ATP synthase subunit b OS=Pseudomonas putida (strain W619) GN=atpF PE=3 SV=1 | |||||||
| ATPF_PSEP1 |
193 | 16959 | 8 (7) | 4 (3) | 0.92 | ||
| ATP synthase subunit b OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpF PE=3 SV=1 | |||||||
| ATPF_PSEE4 |
193 | 17016 | 8 (7) | 4 (3) | 0.92 | ||
| ATP synthase subunit b OS=Pseudomonas entomophila (strain L48) GN=atpF PE=3 SV=1 | |||||||
8 peptide matches (7 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 228 |
516.3041 | 515.2968 | 515.2955 | 2.60 | 1 | 11 | 1.4 | 2Score > 47 indicates identity Score > 25 indicates homology |
K.LAAEI.- | ||
| 1181 |
508.2646 | 1014.5146 | 1014.5094 | 5.17 | 0 | 50 | 0.0084 | 1Score > 41 indicates identity |
U | K.IADGLDAANR.A | |
| 1520 |
1170.6503 | 1169.6430 | 1169.6404 | 2.22 | 1 | 99 | 6.1e-008 | 1Score > 40 indicates identity |
U | R.AQVGALAVGGAEK.I | |
| 1521 |
1 |
585.8293 | 1169.6440 | 1169.6404 | 3.10 | 1 | 58 | 0.00088 | 1Score > 40 indicates identity |
U | R.AQVGALAVGGAEK.I |
| 2858 |
602.9988 | 1805.9746 | 1805.9635 | 6.11 | 1 | 54 | 1.9e-005 | 1Score > 37 indicates identity Score > 20 indicates homology |
U | K.ILGATIDQNAHAELVNK.L | |
| 2860 |
603.3317 | 1806.9733 | 1806.9475 | 14.2 | 1 | 52 | 0.0018 | 1Score > 37 indicates identity |
U | K.ILGATIDQNAHAELVNK.L + Deamidated (NQ) | |
| 2861 |
603.3320 | 1806.9742 | 1806.9475 | 14.7 | 1 | 45 | 0.0013 | 1Score > 37 indicates identity Score > 28 indicates homology |
U | K.ILGATIDQNAHAELVNK.L + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| ATPF_PSE14 |
95 | 16988 | 28.1 | ||
| ATP synthase subunit b OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpF PE=3 SV=1 | |||||
| 3 samesets of ATPF_PSE14 | |||||
| ATPF_PSESM |
95 | 17018 | |||
| ATP synthase subunit b OS=Pseudomonas syringae pv. tomato GN=atpF PE=3 SV=1 | |||||
| ATPF_PSEU2 |
95 | 16988 | |||
| ATP synthase subunit b OS=Pseudomonas syringae pv. syringae (strain B728a) GN=atpF PE=3 SV=1 | |||||
| ATPF_PSEF5 |
95 | 16981 | |||
| ATP synthase subunit b OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=atpF PE=3 SV=1 | |||||
| ATPF_PSEA7 |
50 | 17003 | 28.1 | ||
| ATP synthase subunit b OS=Pseudomonas aeruginosa (strain PA7) GN=atpF PE=3 SV=1 | |||||
| 2 samesets of ATPF_PSEA7 | |||||
| ATPF_PSEAB |
50 | 17003 | |||
| ATP synthase subunit b OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=atpF PE=3 SV=1 | |||||
| ATPF_PSEAE |
50 | 17003 | |||
| ATP synthase subunit b OS=Pseudomonas aeruginosa GN=atpF PE=3 SV=1 | |||||
29| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL16_PSEP1 | 184 | 50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1 |
30| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS16_PSEP1 | 179 | 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1 |
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