MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 sp |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 22:50:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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21| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARPB_PSEPU | 217 | Antibiotic efflux pump membrane transporter arpB OS=Pseudomonas putida GN=arpB PE=2 SV=1 |
22| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL5_PSEE4 | 215 | 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1 | |
| 2 | RL5_FLAPJ | 47 | 50S ribosomal protein L5 OS=Flavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) GN=rplE PE=3 SV=1 | |
23| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL11_PSEP1 | 215 | 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1 |
24| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOA_PSEPK | 211 | DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1 |
25| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL1_PSEE4 | 210 | 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1 |
26| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS20_PSE14 | 201 | 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1 |
27| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPG_PSEPG | 200 | ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 27.1 | ATPG_PSEPG |
200 | 31653 | 13 (10) | 10 (9) | 1.02 | |
| ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1 | |||||||
| 2 samesets of ATPG_PSEPG | |||||||
| ATPG_PSEPK |
200 | 31558 | 13 (10) | 10 (9) | 1.03 | ||
| ATP synthase gamma chain OS=Pseudomonas putida (strain KT2440) GN=atpG PE=3 SV=1 | |||||||
| ATPG_PSEP1 |
200 | 31558 | 13 (10) | 10 (9) | 1.03 | ||
| ATP synthase gamma chain OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpG PE=3 SV=1 | |||||||
13 peptide matches (11 non-duplicate, 2 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 243 |
531.3516 | 530.3443 | 530.3428 | 2.92 | 0 | 29 | 0.31 | 1Score > 38 indicates identity Score > 37 indicates homology |
K.IASIK.S | ||
| 566 |
373.7261 | 745.4376 | 745.4334 | 5.71 | 0 | 44 | 0.007 | 1Score > 39 indicates identity Score > 35 indicates homology |
U | R.LSVVSNK.F | |
| 1192 |
510.7921 | 1019.5696 | 1019.5651 | 4.45 | 1 | 32 | 0.039 | 1Score > 39 indicates identity Score > 30 indicates homology |
U | K.ELQLIYNK.A | |
| 1245 |
523.2935 | 1044.5724 | 1044.5638 | 8.33 | 1 | 30 | 0.03 | 1Score > 41 indicates identity Score > 27 indicates homology |
U | K.ELLDGLMVR.Y | |
| 1277 |
533.7819 | 1065.5492 | 1065.5455 | 3.54 | 0 | 61 | 0.00022 | 1Score > 40 indicates identity Score > 37 indicates homology |
U | R.AGYIVVSSDR.G | |
| 1426 |
566.2876 | 1130.5606 | 1130.5567 | 3.45 | 0 | 40 | 0.045 | 1Score > 42 indicates identity Score > 39 indicates homology |
U | K.NATDNAGDLIK.E | |
| 1474 |
576.7941 | 1151.5736 | 1151.5645 | 7.96 | 0 | 64 | 0.00028 | 1Score > 41 indicates identity Score > 41 indicates homology |
U | K.VMLDAYLDGR.I | |
| 2333 |
525.2427 | 1572.7063 | 1572.6997 | 4.16 | 0 | 22 | 0.041 | 1Score > 39 indicates identity Score > 21 indicates homology |
U | K.HHWDYLYEPDAK.E | |
| 2359 |
791.4130 | 1580.8114 | 1580.8059 | 3.50 | 1 | 69 | 9.5e-006 | 1Score > 40 indicates identity Score > 31 indicates homology |
U | R.QVIGHLANANPEYR.H | |
| 2360 |
1 |
527.9451 | 1580.8135 | 1580.8059 | 4.78 | 1 | 48 | 9.8e-005 | 1Score > 40 indicates identity Score > 20 indicates homology |
U | R.QVIGHLANANPEYR.H |
| 4120 |
1 |
913.1668 | 2736.4786 | 2736.4334 | 16.5 | 1 | 64 | 5.8e-006 | 1Score > 33 indicates identity Score > 24 indicates homology |
U | R.IFGGNVVAAISHLGEEPSINDLIGSVK.V + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| ATPG_PSEE4 |
196 | 31602 | 27.1 | ||
| ATP synthase gamma chain OS=Pseudomonas entomophila (strain L48) GN=atpG PE=3 SV=1 | |||||
| 1 sameset of ATPG_PSEE4 | |||||
| ATPG_PSEPW |
196 | 31545 | |||
| ATP synthase gamma chain OS=Pseudomonas putida (strain W619) GN=atpG PE=3 SV=1 | |||||
| ATPG_PSE14 |
131 | 31458 | 27.1 | ||
| ATP synthase gamma chain OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpG PE=3 SV=1 | |||||
| 2 samesets of ATPG_PSE14 | |||||
| ATPG_PSEPF |
131 | 31518 | |||
| ATP synthase gamma chain OS=Pseudomonas fluorescens (strain Pf0-1) GN=atpG PE=3 SV=1 | |||||
| ATPG_PSEU2 |
131 | 31458 | |||
| ATP synthase gamma chain OS=Pseudomonas syringae pv. syringae (strain B728a) GN=atpG PE=3 SV=1 | |||||
| ATPG_PSEF5 |
106 | 31532 | 27.1 | ||
| ATP synthase gamma chain OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=atpG PE=3 SV=1 | |||||
| 1 sameset of ATPG_PSEF5 | |||||
| ATPG_PSEFS |
106 | 31488 | |||
| ATP synthase gamma chain OS=Pseudomonas fluorescens (strain SBW25) GN=atpG PE=3 SV=1 | |||||
| ATPG_PSEA7 |
91 | 31704 | 27.1 | ||
| ATP synthase gamma chain OS=Pseudomonas aeruginosa (strain PA7) GN=atpG PE=3 SV=1 | |||||
| 3 samesets of ATPG_PSEA7 | |||||
| ATPG_PSEAB |
91 | 31704 | |||
| ATP synthase gamma chain OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=atpG PE=3 SV=1 | |||||
| ATPG_PSEAE |
91 | 31704 | |||
| ATP synthase gamma chain OS=Pseudomonas aeruginosa GN=atpG PE=3 SV=1 | |||||
| ATPG_PSEA8 |
91 | 31704 | |||
| ATP synthase gamma chain OS=Pseudomonas aeruginosa (strain LESB58) GN=atpG PE=3 SV=1 | |||||
| ATPG_PSESM |
67 | 31429 | 27.1 | ||
| ATP synthase gamma chain OS=Pseudomonas syringae pv. tomato GN=atpG PE=3 SV=1 | |||||
| ATPG_PSEMY |
40 | 31680 | 27.1 | ||
| ATP synthase gamma chain OS=Pseudomonas mendocina (strain ymp) GN=atpG PE=3 SV=1 | |||||
| ATPG_BUCAP |
32 | 33471 | 27.1 | ||
| ATP synthase gamma chain OS=Buchnera aphidicola subsp. Schizaphis graminum GN=atpG PE=3 SV=1 | |||||
| 2 samesets of ATPG_BUCAP | |||||
| ATPG_BUCMH |
32 | 33728 | |||
| ATP synthase gamma chain OS=Buchnera aphidicola subsp. Melaphis rhois GN=atpG PE=3 SV=1 | |||||
| ATPG_METCA |
32 | 31756 | |||
| ATP synthase gamma chain OS=Methylococcus capsulatus GN=atpG PE=3 SV=1 | |||||
| ATPG_TERTT |
30 | 31677 | 27.1 | ||
| ATP synthase gamma chain OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=atpG PE=3 SV=1 | |||||
28| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPF_PSEPG | 193 | ATP synthase subunit b OS=Pseudomonas putida (strain GB-1) GN=atpF PE=3 SV=1 |
29| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL16_PSEP1 | 184 | 50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1 |
30| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS16_PSEP1 | 179 | 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1 |
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