MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 sp |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 22:50:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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21| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARPB_PSEPU | 217 | Antibiotic efflux pump membrane transporter arpB OS=Pseudomonas putida GN=arpB PE=2 SV=1 |
22| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL5_PSEE4 | 215 | 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1 | |
| 2 | RL5_FLAPJ | 47 | 50S ribosomal protein L5 OS=Flavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) GN=rplE PE=3 SV=1 | |
23| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL11_PSEP1 | 215 | 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1 |
24| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOA_PSEPK | 211 | DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1 |
25| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL1_PSEE4 | 210 | 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 25.1 | RL1_PSEE4 |
210 | 24208 | 4 (4) | 3 (3) | 0.44 | |
| 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1 | |||||||
| 3 samesets of RL1_PSEE4 | |||||||
| RL1_PSEP1 |
210 | 24236 | 4 (4) | 3 (3) | 0.44 | ||
| 50S ribosomal protein L1 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplA PE=3 SV=1 | |||||||
| RL1_PSEPG |
210 | 24180 | 4 (4) | 3 (3) | 0.44 | ||
| 50S ribosomal protein L1 OS=Pseudomonas putida (strain GB-1) GN=rplA PE=3 SV=1 | |||||||
| RL1_PSEPK |
210 | 24236 | 4 (4) | 3 (3) | 0.44 | ||
| 50S ribosomal protein L1 OS=Pseudomonas putida (strain KT2440) GN=rplA PE=3 SV=1 | |||||||
4 peptide matches (4 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1635 |
611.8700 | 1221.7254 | 1221.7194 | 4.97 | 0 | 50 | 9.8e-005 | 1Score > 33 indicates identity Score > 23 indicates homology |
U | R.VVGQLGQVLGPR.G | |
| 2824 |
897.4581 | 1792.9016 | 1792.8996 | 1.16 | 1 | 43 | 0.00019 | 1Score > 40 indicates identity Score > 19 indicates homology |
U | K.FVESYDIAVNLGVDPR.K | |
| 2992 |
629.3372 | 1884.9898 | 1884.9694 | 10.8 | 1 | 89 | 9.6e-008 | 1Score > 38 indicates identity Score > 32 indicates homology |
U | R.VAVFTQGPAAEAALAAGADR.V | |
| 2993 |
944.0002 | 1885.9858 | 1885.9534 | 17.2 | 1 | 105 | 1.9e-009 | 1Score > 39 indicates identity Score > 30 indicates homology |
U | R.VAVFTQGPAAEAALAAGADR.V + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| RL1_PSE14 |
188 | 24120 | 25.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplA PE=3 SV=1 | |||||
| 4 samesets of RL1_PSE14 | |||||
| RL1_PSEF5 |
188 | 24047 | |||
| 50S ribosomal protein L1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplA PE=3 SV=1 | |||||
| RL1_PSEPW |
188 | 24112 | |||
| 50S ribosomal protein L1 OS=Pseudomonas putida (strain W619) GN=rplA PE=3 SV=1 | |||||
| RL1_PSESM |
188 | 24163 | |||
| 50S ribosomal protein L1 OS=Pseudomonas syringae pv. tomato GN=rplA PE=3 SV=1 | |||||
| RL1_PSEU2 |
188 | 24176 | |||
| 50S ribosomal protein L1 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplA PE=3 SV=1 | |||||
| RL1_AZOVD |
163 | 24230 | 25.1 | ||
| 50S ribosomal protein L1 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplA PE=3 SV=1 | |||||
| 2 samesets of RL1_AZOVD | |||||
| RL1_PSEFS |
163 | 24061 | |||
| 50S ribosomal protein L1 OS=Pseudomonas fluorescens (strain SBW25) GN=rplA PE=3 SV=1 | |||||
| RL1_PSEPF |
163 | 24193 | |||
| 50S ribosomal protein L1 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplA PE=3 SV=1 | |||||
| RL1_ACTP2 |
50 | 23932 | 25.1 | ||
| 50S ribosomal protein L1 OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=rplA PE=3 SV=1 | |||||
| 60 samesets of RL1_ACTP2 |
|||||
26| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS20_PSE14 | 201 | 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1 |
27| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPG_PSEPG | 200 | ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1 |
28| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPF_PSEPG | 193 | ATP synthase subunit b OS=Pseudomonas putida (strain GB-1) GN=atpF PE=3 SV=1 |
29| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL16_PSEP1 | 184 | 50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1 |
30| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS16_PSEP1 | 179 | 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1 |
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