MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 230)

Page: Previous 1 2 3 4 5 6 7 8 … 23 Next 

+21

Accession Score Description
1 ARPB_PSEPU 217 Antibiotic efflux pump membrane transporter arpB OS=Pseudomonas putida GN=arpB PE=2 SV=1

+22

Accession Score Description
Family member distances as a dendrogram 1 RL5_PSEE4 215 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
2 RL5_FLAPJ 47 50S ribosomal protein L5 OS=Flavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) GN=rplE PE=3 SV=1

+23

Accession Score Description
1 RL11_PSEP1 215 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1

-24

Accession Score Description
1 RPOA_PSEPK 211 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1
Score Mass Matches Sequences emPAI
24.1 RPOA_PSEPK 211 36721 12 (10) 8 (7) 0.84
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1
2 samesets of RPOA_PSEPK
RPOA_PSEP1 211 36721 12 (10) 8 (7) 0.84
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoA PE=3 SV=1
RPOA_PSEE4 211 36721 12 (10) 8 (7) 0.84
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas entomophila (strain L48) GN=rpoA PE=3 SV=1

-12 peptide matches (11 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1092 +1 485.7845 969.5544 969.5495 5.14 1 41 0.033 +1Score > 39 indicates identity U K.ITLEPLER.G
1219   517.7868 1033.5590 1033.5556 3.32 1 38 0.021 +1Score > 40 indicates identity
Score > 34 indicates homology		 U R.IAYVVENAR.V
1449   570.8093 1139.6040 1139.5975 5.76 0 39 0.0033 +1Score > 39 indicates identity
Score > 26 indicates homology		 U R.LDNWPPASLK.K
1451   571.8083 1141.6020 1141.5992 2.46 0 36 0.029 +1Score > 40 indicates identity
Score > 33 indicates homology		 U R.GFGHTLGNALR.R
1452   381.5413 1141.6021 1141.5992 2.48 0 41 0.0048 +1Score > 40 indicates identity
Score > 30 indicates homology		 U R.GFGHTLGNALR.R
1658   616.8403 1231.6660 1231.6561 8.09 0 59 9.4e-005 +1Score > 40 indicates identity
Score > 32 indicates homology		 U R.LQLDASFSPVR.R
1694   417.5680 1249.6822 1249.6779 3.42 0 35 0.0046 +1Score > 39 indicates identity
Score > 24 indicates homology		 U R.HIDVQVVSPTR.A
1695   625.8488 1249.6830 1249.6779 4.12 0 64 4.3e-005 +1Score > 39 indicates identity
Score > 33 indicates homology		 U R.HIDVQVVSPTR.A
1956   463.5959 1387.7659 1387.7572 6.26 1 12 1.2 +2Score > 38 indicates identity
Score > 25 indicates homology		 U R.LQLDASFSPVRR.I
2323   523.2775 1566.8107 1566.8042 4.15 1 75 4.4e-006 +1Score > 40 indicates identity
Score > 34 indicates homology		 U K.AENIYYIGDLIQR.T
2324   784.4152 1566.8158 1566.8042 7.45 1 64 1.2e-005 +1Score > 39 indicates identity
Score > 27 indicates homology		 U K.AENIYYIGDLIQR.T

8 subsets and intersections (186 subset proteins in total)

Score Mass Subset of
RPOA_PSE14 184 36765 24.1
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpoA PE=3 SV=1
5 samesets of RPOA_PSE14
RPOA_PSEPF 184 36723
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpoA PE=3 SV=1
RPOA_PSEPU 184 36695
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida GN=rpoA PE=3 SV=1
RPOA_PSESM 184 36765
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. tomato GN=rpoA PE=3 SV=1
RPOA_PSEU2 184 36765
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpoA PE=3 SV=1
RPOA_PSEF5 184 36721
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpoA PE=3 SV=1
RPOA_PSEU5 179 36620 24.1
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas stutzeri (strain A1501) GN=rpoA PE=3 SV=1
RPOA_PSEA7 166 36741 24.1
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa (strain PA7) GN=rpoA PE=3 SV=1
3 samesets of RPOA_PSEA7
RPOA_PSEAB 166 36741
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpoA PE=3 SV=1
RPOA_PSEAE 166 36741
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa GN=rpoA PE=3 SV=2
RPOA_PSEMY 166 36661
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas mendocina (strain ymp) GN=rpoA PE=3 SV=1
RPOA_SACD2 151 36391 24.1
DNA-directed RNA polymerase subunit alpha OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpoA PE=3 SV=1
RPOA_AZOSB 123 35767 24.1
DNA-directed RNA polymerase subunit alpha OS=Azoarcus sp. (strain BH72) GN=rpoA PE=3 SV=1
5 samesets of RPOA_AZOSB
RPOA_AZOSE 123 35666
DNA-directed RNA polymerase subunit alpha OS=Azoarcus sp. (strain EbN1) GN=rpoA PE=3 SV=1
RPOA_CELJU 123 36326
DNA-directed RNA polymerase subunit alpha OS=Cellvibrio japonicus (strain Ueda107) GN=rpoA PE=3 SV=1
RPOA_CHRSD 123 37113
DNA-directed RNA polymerase subunit alpha OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpoA PE=3 SV=1
RPOA_LEPCP 123 36343
DNA-directed RNA polymerase subunit alpha OS=Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) GN=rpoA PE=3 SV=1
RPOA_THISH 123 36890
DNA-directed RNA polymerase subunit alpha OS=Thioalkalivibrio sp. (strain HL-EbGR7) GN=rpoA PE=3 SV=1
RPOA_ACIAC 109 36282 24.1
DNA-directed RNA polymerase subunit alpha OS=Acidovorax avenae subsp. citrulli (strain AAC00-1) GN=rpoA PE=3 SV=1
+52 samesets of RPOA_ACIAC
RPOA_BLOFL 50 37420 24.1
DNA-directed RNA polymerase subunit alpha OS=Blochmannia floridanus GN=rpoA PE=3 SV=1
3 samesets of RPOA_BLOFL
RPOA_WIGBR 50 37133
DNA-directed RNA polymerase subunit alpha OS=Wigglesworthia glossinidia brevipalpis GN=rpoA PE=3 SV=1
RPOA_BUCBP 50 37453
DNA-directed RNA polymerase subunit alpha OS=Buchnera aphidicola subsp. Baizongia pistaciae GN=rpoA PE=3 SV=1
RPOA_BUCCC 50 37214
DNA-directed RNA polymerase subunit alpha OS=Buchnera aphidicola subsp. Cinara cedri GN=rpoA PE=3 SV=1
RPOA1_PSYIN 45 36427 24.1
DNA-directed RNA polymerase subunit alpha 1 OS=Psychromonas ingrahamii (strain 37) GN=rpoA1 PE=3 SV=1
+110 samesets of RPOA1_PSYIN

+25

Accession Score Description
1 RL1_PSEE4 210 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+26

Accession Score Description
1 RS20_PSE14 201 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

+27

Accession Score Description
1 ATPG_PSEPG 200 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

+28

Accession Score Description
1 ATPF_PSEPG 193 ATP synthase subunit b OS=Pseudomonas putida (strain GB-1) GN=atpF PE=3 SV=1

+29

Accession Score Description
1 RL16_PSEP1 184 50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1

+30

Accession Score Description
1 RS16_PSEP1 179 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1
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