MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 230)

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+21

Accession Score Description
1 ARPB_PSEPU 217 Antibiotic efflux pump membrane transporter arpB OS=Pseudomonas putida GN=arpB PE=2 SV=1

+22

Accession Score Description
Family member distances as a dendrogram 1 RL5_PSEE4 215 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
2 RL5_FLAPJ 47 50S ribosomal protein L5 OS=Flavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) GN=rplE PE=3 SV=1

-23

Accession Score Description
1 RL11_PSEP1 215 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1
Score Mass Matches Sequences emPAI
23.1 RL11_PSEP1 215 14914 9 (7) 6 (6) 1.82
50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1
3 samesets of RL11_PSEP1
RL11_PSEPG 215 14914 9 (7) 6 (6) 1.82
50S ribosomal protein L11 OS=Pseudomonas putida (strain GB-1) GN=rplK PE=3 SV=1
RL11_PSEPK 215 14914 9 (7) 6 (6) 1.82
50S ribosomal protein L11 OS=Pseudomonas putida (strain KT2440) GN=rplK PE=3 SV=1
RL11_PSEPW 215 14914 9 (7) 6 (6) 1.82
50S ribosomal protein L11 OS=Pseudomonas putida (strain W619) GN=rplK PE=3 SV=1

-9 peptide matches (8 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
820   430.2148 858.4150 858.4123 3.16 1 33 0.052 +1Score > 40 indicates identity
Score > 32 indicates homology		 U R.SFTFETK.S
876   444.2476 886.4806 886.4760 5.28 1 50 0.0087 +1Score > 42 indicates identity U R.AQLEDIAK.A
946   915.5532 914.5459 914.5437 2.46 0 41 0.04 +1Score > 39 indicates identity U K.STPASVLLK.K
947   458.2817 914.5488 914.5437 5.66 0 24 0.077 +1Score > 39 indicates identity
Score > 26 indicates homology		 U K.STPASVLLK.K
2044   715.3705 1428.7264 1428.7209 3.91 0 88 2.6e-007 +1Score > 41 indicates identity
Score > 35 indicates homology		 U K.QADLTAADLDAAVR.T
2045   477.2649 1428.7729 1428.7685 3.08 0 26 0.022 +1Score > 40 indicates identity
Score > 22 indicates homology		 U K.AAGLTSGSARPNTVK.V
3433   1073.5706 2145.1266 2145.0954 14.6 1 93 7.2e-009 +1Score > 38 indicates identity
Score > 24 indicates homology		 U R.TQGQEAGLPTPVIITVYSDR.S + Deamidated (NQ)
3434 +1 716.0533 2145.1381 2145.0954 19.9 1 71 1.3e-006 +1Score > 37 indicates identity
Score > 25 indicates homology		 U R.TQGQEAGLPTPVIITVYSDR.S + Deamidated (NQ)

+11 subsets and intersections (57 subset proteins in total)

+24

Accession Score Description
1 RPOA_PSEPK 211 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+25

Accession Score Description
1 RL1_PSEE4 210 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+26

Accession Score Description
1 RS20_PSE14 201 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

+27

Accession Score Description
1 ATPG_PSEPG 200 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

+28

Accession Score Description
1 ATPF_PSEPG 193 ATP synthase subunit b OS=Pseudomonas putida (strain GB-1) GN=atpF PE=3 SV=1

+29

Accession Score Description
1 RL16_PSEP1 184 50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1

+30

Accession Score Description
1 RS16_PSEP1 179 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1
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