MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 230)

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+21

Accession Score Description
1 ARPB_PSEPU 217 Antibiotic efflux pump membrane transporter arpB OS=Pseudomonas putida GN=arpB PE=2 SV=1

-22

Accession Score Description
Family member distances as a dendrogram 1 RL5_PSEE4 215 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
2 RL5_FLAPJ 47 50S ribosomal protein L5 OS=Flavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) GN=rplE PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
RL5_PSEE4 215 20385 18 (10) 10 (5) 0.92
50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
4 samesets of RL5_PSEE4
RL5_PSEP1 215 20316 18 (10) 10 (5) 0.92
50S ribosomal protein L5 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplE PE=3 SV=1
RL5_PSEPG 215 20316 18 (10) 10 (5) 0.92
50S ribosomal protein L5 OS=Pseudomonas putida (strain GB-1) GN=rplE PE=3 SV=1
RL5_PSEPK 215 20316 18 (10) 10 (5) 0.92
50S ribosomal protein L5 OS=Pseudomonas putida (strain KT2440) GN=rplE PE=3 SV=1
RL5_PSEPW 215 20399 18 (10) 10 (5) 0.92
50S ribosomal protein L5 OS=Pseudomonas putida (strain W619) GN=rplE PE=3 SV=1
RL5_FLAPJ 47 20315 2 (2) 2 (2) 0.24
50S ribosomal protein L5 OS=Flavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) GN=rplE PE=3 SV=1

-19 peptide matches (13 non-duplicate, 6 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
272   581.2704 580.2631 580.2605 4.47 0 19 2.3 +6Score > 40 indicates identity
Score > 35 indicates homology		 X K.SFDGR.G
331   311.6825 621.3504 621.3486 2.97 0 18 1.7 +3Score > 38 indicates identity
Score > 33 indicates homology		 X K.SIAGFK.V
393   666.3480 665.3407 665.3384 3.43 0 42 0.052 +4Score > 41 indicates identity U X R.DFSGIK.A
425   340.6805 679.3464 679.3442 3.32 1 17 1.5 +3Score > 40 indicates identity
Score > 31 indicates homology		 X K.FPFRN.-
573   375.7307 749.4468 749.4436 4.39 1 24 0.3 +1Score > 36 indicates identity
Score > 31 indicates homology		 X R.KSIAGFK.V
865   882.5818 881.5745 881.5698 5.35 0 2 1 +4Score > 30 indicates identity
Score > 15 indicates homology		 U X R.LLAISLPR.V
867 +3 441.7954 881.5762 881.5698 7.30 0 50 0.00034 +1Score > 28 indicates identity U X R.LLAISLPR.V
935   304.8425 911.5057 911.5018 4.30 1 28 0.078 +1Score > 38 indicates identity
Score > 30 indicates homology		 U X R.AFKFPFR.N
1099   487.2276 972.4406 972.4375 3.25 1 45 0.012 +1Score > 39 indicates identity U X X MYEFLDR.L
1510 +2 581.3317 1160.6488 1160.6401 7.53 0 52 0.0029 +1Score > 39 indicates identity U X R.GLDITLTTTAR.S
1809   658.8898 1315.7650 1315.7612 2.90 0 53 0.00023 +1Score > 35 indicates identity
Score > 29 indicates homology		 U X K.ITGQKPVVTFAR.K
1810 +1 439.5958 1315.7656 1315.7612 3.30 0 54 6.6e-005 +1Score > 35 indicates identity
Score > 25 indicates homology		 U X K.ITGQKPVVTFAR.K
2052   716.3847 1430.7548 1430.7439 7.65 1 78 9.9e-006 +1Score > 40 indicates identity U X K.ITLNMGLGEAIGDK.K

5 subsets and intersections (152 subset proteins in total)

Score Mass Subset of
RL5_PSEMY 97 20437 22.1
50S ribosomal protein L5 OS=Pseudomonas mendocina (strain ymp) GN=rplE PE=3 SV=1
1 sameset of RL5_PSEMY
RL5_PSEU5 97 20384
50S ribosomal protein L5 OS=Pseudomonas stutzeri (strain A1501) GN=rplE PE=3 SV=1
RL5_PSE14 84 20460 22.1
50S ribosomal protein L5 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplE PE=3 SV=1
4 samesets of RL5_PSE14
RL5_PSEF5 84 20474
50S ribosomal protein L5 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplE PE=3 SV=1
RL5_PSEPF 84 20474
50S ribosomal protein L5 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplE PE=3 SV=1
RL5_PSESM 84 20407
50S ribosomal protein L5 OS=Pseudomonas syringae pv. tomato GN=rplE PE=3 SV=1
RL5_PSEU2 84 20460
50S ribosomal protein L5 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplE PE=3 SV=1
RL5_AZOVD 58 20433 22.1
50S ribosomal protein L5 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplE PE=3 SV=1
4 samesets of RL5_AZOVD
RL5_PSEA7 58 20393
50S ribosomal protein L5 OS=Pseudomonas aeruginosa (strain PA7) GN=rplE PE=3 SV=1
RL5_PSEA8 58 20379
50S ribosomal protein L5 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplE PE=3 SV=1
RL5_PSEAB 58 20379
50S ribosomal protein L5 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplE PE=3 SV=1
RL5_PSEAE 58 20379
50S ribosomal protein L5 OS=Pseudomonas aeruginosa GN=rplE PE=3 SV=1
ATPD_CLOAB 45 20781 22.1
ATP synthase subunit delta OS=Clostridium acetobutylicum GN=atpH PE=3 SV=1
+119 samesets of ATPD_CLOAB
AROC_CAMJ8 42 0 22.2
description
19 samesets of AROC_CAMJ8
RBM25_HUMAN 42 0
description
RBM25_MOUSE 42 0
description
AROC_CAMJD 42 0
description
AROC_CAMJE 42 0
description
AROC_CAMJJ 42 0
description
AROC_CAMJR 42 0
description
DEF_THETH 42 0
description
GFT_ALLCE 42 0
description
GGR_THEAC 42 0
description
GGR_THEVO 42 0
description
MPGP_PYRAB 42 0
description
MRAW_CAMJD 42 0
description
MURG_PORGI 42 0
description
GSTO1_HUMAN 42 0
description
GSTO1_RAT 42 0
description
DEF_THET2 42 0
description
MURG_PORG3 42 0
description
DEF_THET8 42 0
description
YGP9_YEAST 42 0
description

+23

Accession Score Description
1 RL11_PSEP1 215 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1

+24

Accession Score Description
1 RPOA_PSEPK 211 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+25

Accession Score Description
1 RL1_PSEE4 210 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+26

Accession Score Description
1 RS20_PSE14 201 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

+27

Accession Score Description
1 ATPG_PSEPG 200 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

+28

Accession Score Description
1 ATPF_PSEPG 193 ATP synthase subunit b OS=Pseudomonas putida (strain GB-1) GN=atpF PE=3 SV=1

+29

Accession Score Description
1 RL16_PSEP1 184 50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1

+30

Accession Score Description
1 RS16_PSEP1 179 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1
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