MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 230)

Page: Previous 1 2 3 4 5 6 7 8 … 23 Next 

-21

Accession Score Description
1 ARPB_PSEPU 217 Antibiotic efflux pump membrane transporter arpB OS=Pseudomonas putida GN=arpB PE=2 SV=1
Score Mass Matches Sequences emPAI
21.1 ARPB_PSEPU 217 112893 10 (7) 9 (6) 0.15
Antibiotic efflux pump membrane transporter arpB OS=Pseudomonas putida GN=arpB PE=2 SV=1
3 samesets of ARPB_PSEPU
MEPB_PSEPU 217 112935 10 (7) 9 (6) 0.15
Multidrug/solvent efflux pump membrane transporter mepB OS=Pseudomonas putida GN=mepB PE=1 SV=1
TTGB_PSEPK 217 112935 10 (7) 9 (6) 0.15
Probable efflux pump membrane transporter ttgB OS=Pseudomonas putida (strain KT2440) GN=ttgB PE=1 SV=1
TTGB_PSEPU 217 112907 10 (7) 9 (6) 0.15
Toluene efflux pump membrane transporter ttgB OS=Pseudomonas putida GN=ttgB PE=2 SV=2

-10 peptide matches (10 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
764   421.7436 841.4726 841.4698 3.42 0 29 0.22 +1Score > 38 indicates identity
Score > 35 indicates homology		 R.IWLDPAK.L
1246   523.8008 1045.5870 1045.5808 5.97 0 18 0.18 +1Score > 40 indicates identity
Score > 23 indicates homology		 U K.FQLTPVDVK.T
1328   544.2642 1086.5138 1086.5036 9.46 0 28 0.12 +1Score > 40 indicates identity
Score > 31 indicates homology		 U K.GGFFGWFNR.N
1461   573.3132 1144.6118 1144.6088 2.68 0 48 0.00092 +1Score > 41 indicates identity
Score > 30 indicates homology		 U K.LATGANALDTAK.A
1550   394.5345 1180.5817 1180.5778 3.29 0 28 0.019 +1Score > 41 indicates identity
Score > 23 indicates homology		 U R.AQQHFFTFR.D
3188   1010.0805 2018.1464 2018.1160 15.1 1 70 7.8e-007 +1Score > 32 indicates identity
Score > 22 indicates homology		 U K.LNLATPLLPQEVQQQGIR.V + Deamidated (NQ)
3189   673.7239 2018.1499 2018.1160 16.8 1 69 1e-006 +1Score > 31 indicates identity
Score > 21 indicates homology		 U K.LNLATPLLPQEVQQQGIR.V + Deamidated (NQ)
4137   919.8059 2756.3959 2756.3617 12.4 1 35 0.00069 +1Score > 37 indicates identity
Score > 16 indicates homology		 U K.ILSAVRPNGLNDEPQYQLTIDDER.A + Deamidated (NQ)
4398   1032.5490 3094.6252 3094.5822 13.9 0 59 0.00017 +1Score > 34 indicates identity U R.ASALGVTIADINNTLSIALGASYVNDFIDR.G + Deamidated (NQ)
4457   1059.2621 3174.7645 3174.7248 12.5 0 58 6.7e-005 +1Score > 29 indicates identity U K.TAVAAQNVQVSSGQLGGLPALPGTQLNATIIGK.T + Deamidated (NQ)

1 subset or intersection (2 subset proteins in total)

Score Mass Subset of
ACRF_ECOLI 48 111554 21.1
Acriflavine resistance protein F OS=Escherichia coli (strain K12) GN=acrF PE=3 SV=2
1 sameset of ACRF_ECOLI
MEXB_PSEAE 48 112890
Multidrug resistance protein mexB OS=Pseudomonas aeruginosa GN=mexB PE=1 SV=2

+22

Accession Score Description
Family member distances as a dendrogram 1 RL5_PSEE4 215 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
2 RL5_FLAPJ 47 50S ribosomal protein L5 OS=Flavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) GN=rplE PE=3 SV=1

+23

Accession Score Description
1 RL11_PSEP1 215 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1

+24

Accession Score Description
1 RPOA_PSEPK 211 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+25

Accession Score Description
1 RL1_PSEE4 210 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+26

Accession Score Description
1 RS20_PSE14 201 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

+27

Accession Score Description
1 ATPG_PSEPG 200 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

+28

Accession Score Description
1 ATPF_PSEPG 193 ATP synthase subunit b OS=Pseudomonas putida (strain GB-1) GN=atpF PE=3 SV=1

+29

Accession Score Description
1 RL16_PSEP1 184 50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1

+30

Accession Score Description
1 RS16_PSEP1 179 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1
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