MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 201–210 (out of 230)


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+201

Accession Score Description
1 NUOCD_PSESM 29 NADH-quinone oxidoreductase subunit C/D OS=Pseudomonas syringae pv. tomato GN=nuoC PE=3 SV=1

+202

Accession Score Description
1 ZIPA_PSEPG 29 Cell division protein zipA homolog OS=Pseudomonas putida (strain GB-1) GN=zipA PE=3 SV=1

-203

Accession Score Description
1 Y018_CAUSK 28 Putative NADH dehydrogenase/NAD(P)H nitroreductase Caul_0018 OS=Caulobacter sp. (strain K31) GN=Caul_0018 PE=3 SV=1
Score Mass Matches Sequences emPAI
203.1 Y018_CAUSK 28 20954 2 (1) 2 (1) 0.11
Putative NADH dehydrogenase/NAD(P)H nitroreductase Caul_0018 OS=Caulobacter sp. (strain K31) GN=Caul_0018 PE=3 SV=1

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2173   749.3975 1496.7804 1496.7623 12.1 0 28 0.014 +1Score > 40 indicates identity
Score > 22 indicates homology
U R.NSSLQGGYFILAAR.A + Deamidated (NQ)
2952   932.4613 1862.9080 1862.8720 19.3 0 1 29 +6Score > 40 indicates identity
Score > 28 indicates homology
U R.ALGLDVGPMSGFDNAGVDK.E + Deamidated (NQ)

+204

Accession Score Description
1 F16A2_ACAM1 28 Fructose-1,6-bisphosphatase class 1 2 OS=Acaryochloris marina (strain MBIC 11017) GN=fbp2 PE=3 SV=1

+205

Accession Score Description
1 RL25_LARHH 27 50S ribosomal protein L25 OS=Laribacter hongkongensis (strain HLHK9) GN=rplY PE=3 SV=1

+206

Accession Score Description
1 END4_THEVO 27 Probable endonuclease 4 OS=Thermoplasma volcanium GN=nfo PE=3 SV=1

+207

Accession Score Description
1 PYRH_CAMC1 26 Uridylate kinase OS=Campylobacter concisus (strain 13826) GN=pyrH PE=3 SV=1

+208

Accession Score Description
1 EX7L_CHLT2 26 Exodeoxyribonuclease 7 large subunit OS=Chlamydia trachomatis (strain L2/434/Bu / ATCC VR-902B) GN=xseA PE=3 SV=1

+209

Accession Score Description
1 MUTL_ECOBW 26 DNA mismatch repair protein mutL OS=Escherichia coli (strain K12 / BW2952) GN=mutL PE=3 SV=1

+210

Accession Score Description
1 RS12_AZOVD 26 30S ribosomal protein S12 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsL PE=3 SV=1
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