MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 230)


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+11

Accession Score Description
1 ODB2_PSEPU 324 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+12

Accession Score Description
1 DHSA_ECOLI 318 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+13

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEE4 298 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1
2 RL4_PSE14 297 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+14

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 297 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
2 CH601_ECOK1 101 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
3 CH60_GEOLS 90 60 kDa chaperonin OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=groL PE=3 SV=1

+15

Accession Score Description
1 RS2_PSEP1 297 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

+16

Accession Score Description
1 ODO2_PSEAE 292 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+17

Accession Score Description
1 SUCC_PSEPK 257 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+18

Accession Score Description
1 EFG1_PSEPK 240 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+19

Accession Score Description
1 ARPA_PSEPU 230 Antibiotic efflux pump periplasmic linker protein arpA OS=Pseudomonas putida GN=arpA PE=2 SV=1

-20

Accession Score Description
1 RL18_PSEP1 226 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
Score Mass Matches Sequences emPAI
20.1 RL18_PSEP1 226 12677 7 (5) 5 (4) 1.38
50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
3 samesets of RL18_PSEP1
RL18_PSEPG 226 12677 7 (5) 5 (4) 1.38
50S ribosomal protein L18 OS=Pseudomonas putida (strain GB-1) GN=rplR PE=3 SV=1
RL18_PSEPK 226 12677 7 (5) 5 (4) 1.38
50S ribosomal protein L18 OS=Pseudomonas putida (strain KT2440) GN=rplR PE=3 SV=1
RL18_PSEPW 226 12665 7 (5) 5 (4) 1.38
50S ribosomal protein L18 OS=Pseudomonas putida (strain W619) GN=rplR PE=3 SV=1

-7 peptide matches (7 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1160   1004.5444 1003.5371 1003.5550 -17.8 0 17 8.1 +6Score > 41 indicates identity
Score > 38 indicates homology
K.VLASASTLDK.D
1161   502.7768 1003.5390 1003.5550 -15.9 0 23 0.43 +1Score > 41 indicates identity
Score > 32 indicates homology
K.VLASASTLDK.D
1389   560.7926 1119.5706 1119.5673 3.01 0 61 0.00017 +1Score > 42 indicates identity
Score > 36 indicates homology
U K.AAGVSQVAFDR.S
1431   567.2758 1132.5370 1132.5360 0.90 0 75 2.4e-005 +1Score > 41 indicates identity U R.DGATGNIDAATK.V
1957   694.8934 1387.7722 1387.7671 3.73 1 104 1.5e-008 +1Score > 38 indicates identity U K.VLASASTLDKDLR.D
2609   564.2859 1689.8359 1689.8322 2.17 0 44 0.0006 +1Score > 41 indicates identity
Score > 24 indicates homology
U R.SSQHIYAQVISADGSK.V
2610   845.9254 1689.8362 1689.8322 2.40 0 74 7.4e-007 +1Score > 41 indicates identity
Score > 25 indicates homology
U R.SSQHIYAQVISADGSK.V

4 subsets and intersections (13 subset proteins in total)

Score Mass Subset of
RL18_PSE14 159 12653 20.1
50S ribosomal protein L18 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplR PE=3 SV=1
3 samesets of RL18_PSE14
RL18_PSEE4 159 12651
50S ribosomal protein L18 OS=Pseudomonas entomophila (strain L48) GN=rplR PE=3 SV=1
RL18_PSESM 159 12653
50S ribosomal protein L18 OS=Pseudomonas syringae pv. tomato GN=rplR PE=3 SV=1
RL18_PSEU2 159 12653
50S ribosomal protein L18 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplR PE=3 SV=1
RL18_PSEA7 104 12711 20.1
50S ribosomal protein L18 OS=Pseudomonas aeruginosa (strain PA7) GN=rplR PE=3 SV=1
4 samesets of RL18_PSEA7
RL18_PSEA8 104 12711
50S ribosomal protein L18 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplR PE=3 SV=1
RL18_PSEAB 104 12711
50S ribosomal protein L18 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplR PE=3 SV=1
RL18_PSEAE 104 12711
50S ribosomal protein L18 OS=Pseudomonas aeruginosa GN=rplR PE=3 SV=1
RL18_TERTT 104 12760
50S ribosomal protein L18 OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=rplR PE=3 SV=1
RL18_PSEF5 97 12692 20.1
50S ribosomal protein L18 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplR PE=3 SV=1
2 samesets of RL18_PSEF5
RL18_PSEFS 97 12708
50S ribosomal protein L18 OS=Pseudomonas fluorescens (strain SBW25) GN=rplR PE=3 SV=1
RL18_PSEPF 97 12692
50S ribosomal protein L18 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplR PE=3 SV=1
RL18_PSEMY 93 12636 20.1
50S ribosomal protein L18 OS=Pseudomonas mendocina (strain ymp) GN=rplR PE=3 SV=1

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