MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 230)

Page: 1 2 3 4 5 6 … 23 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 1648 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_LEGPA 425 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1

-2

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1599 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
5 EFTU_MYCS5 42 Elongation factor Tu OS=Mycoplasma synoviae (strain 53) GN=tuf PE=3 SV=1
3 EFTU_CARRP 146 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
4 EFTU_ANATD 54 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1484 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
EFTU2_PSEPK 1599 43793 98 (70) 18 (15) 4.97
Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
1 sameset of EFTU2_PSEPK
EFTU_PSEE4 1599 43793 98 (70) 18 (15) 4.97
Elongation factor Tu OS=Pseudomonas entomophila (strain L48) GN=tuf1 PE=3 SV=1
EFTU1_PSEPK 1484 43810 92 (66) 18 (15) 4.67
Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
3 samesets of EFTU1_PSEPK
EFTU_PSEPG 1484 43810 92 (66) 18 (15) 4.67
Elongation factor Tu OS=Pseudomonas putida (strain GB-1) GN=tuf1 PE=3 SV=1
EFTU_PSEPW 1484 43810 92 (66) 18 (15) 4.67
Elongation factor Tu OS=Pseudomonas putida (strain W619) GN=tuf1 PE=3 SV=1
EFTU_PSEP1 1484 43810 92 (66) 18 (15) 4.67
Elongation factor Tu OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=tuf PE=3 SV=1
EFTU_CARRP 146 44439 13 (7) 2 (2) 0.16
Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
EFTU_ANATD 54 44208 10 (2) 4 (2) 0.11
Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
1 sameset of EFTU_ANATD
EFTU_CALS8 54 44218 10 (2) 4 (2) 0.11
Elongation factor Tu OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) GN=tuf PE=3 SV=1
EFTU_MYCS5 42 43317 7 (2) 3 (2) 0.11
Elongation factor Tu OS=Mycoplasma synoviae (strain 53) GN=tuf PE=3 SV=1

-118 peptide matches (55 non-duplicate, 63 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 5 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 5 Peptide
289   589.3196 588.3123 588.3119 0.79 0 43 0.029 +1Score > 46 indicates identity
Score > 40 indicates homology		 U X E.NVNLK.V + 2 Deamidated (NQ)
448 +1 345.2011 688.3876 688.3868 1.22 0 32 0.11 +1Score > 43 indicates identity
Score > 35 indicates homology		 X X X R.GTVVTGR  
451 +1 689.3964 688.3891 688.3868 3.36 0 28 0.67 +1Score > 43 indicates identity
Score > 39 indicates homology		 X X X R.GTVVTGR  
471   702.3801 701.3728 701.3708 2.90 1 35 0.11 +1Score > 45 indicates identity
Score > 38 indicates homology		 X X K.LLDEGR.A
472 +1 351.6937 701.3728 701.3708 2.94 1 27 0.31 +1Score > 45 indicates identity
Score > 34 indicates homology		 X X K.LLDEGR.A
622 +3 388.7091 775.4036 775.4017 2.49 0 24 0.07 +1Score > 42 indicates identity
Score > 25 indicates homology		 U X X X X R.HTPFFK  
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_1_20250714114344.raw

Score > 42 indicates identity

Score > 26 indicates homology
624   776.4114 775.4041 775.4017 3.10 0 32 0.014 -1Score > 42 indicates identity
Score > 26 indicates homology		 U X X X X R.HTPFFK  
-1.25 0 11 1.5 2 HVVMFK   + Oxidation (M)
18.5 1 10 2 3 AGKPEMK   + Oxidation (M)
-1.23 0 10 2.1 4 HTMFLK  
8.32 0 10 2.1 5 HTSYIR  
18.5 0 8 3.1 6 QQAGMLL   + Oxidation (M)
18.4 1 8 3.1 7 KAGMPTGV   + Oxidation (M)
18.5 1 8 3.3 8 QMLQEK  
-5.56 0 8 3.6 9 IMMLPR   + Oxidation (M)
-5.56 0 8 3.6 9 MLIMPR   + Oxidation (M)
686 +2 401.2473 800.4800 800.4756 5.54 0 44 0.0051 +1Score > 42 indicates identity
Score > 33 indicates homology		 U X X R.TVGAGVVAK.I
687 +1 801.4874 800.4801 800.4756 5.63 0 51 0.0064 +1Score > 42 indicates identity U X X R.TVGAGVVAK.I
834   867.5073 866.5000 866.4974 3.06 1 54 0.00096 +1Score > 37 indicates identity U X X R.EHILLSR.Q
835 +2 434.2573 866.5000 866.4974 3.08 1 44 0.011 +1Score > 37 indicates identity U X X R.EHILLSR.Q
1038 +1 947.5552 946.5479 946.5447 3.36 0 51 0.00055 +1Score > 38 indicates identity
Score > 31 indicates homology		 U X X K.TTLTAALTR.V
1039 +2 474.2820 946.5494 946.5447 4.97 0 81 9e-007 +1Score > 38 indicates identity
Score > 33 indicates homology		 U X X K.TTLTAALTR.V
1164 +1 503.2565 1004.4984 1004.4961 2.37 1 47 0.018 +1Score > 42 indicates identity U X X K.TIAMEDGLR.F
1193   511.2544 1020.4942 1020.4910 3.20 1 39 0.003 +1Score > 42 indicates identity
Score > 26 indicates homology		 U X X K.TIAMEDGLR.F + Oxidation (M)
1331   544.7812 1087.5478 1087.5444 3.15 1 101 7e-008 +1Score > 42 indicates identity U X X R.AGENCGVLLR.G
1480 +1 1156.6298 1155.6225 1155.6176 4.29 1 67 0.00011 +1Score > 40 indicates identity U X X K.FTAEVYVLSK.E
1482 +8 578.8196 1155.6246 1155.6176 6.13 1 49 0.00088 +1Score > 40 indicates identity
Score > 31 indicates homology		 U X X K.FTAEVYVLSK.E
1660 +2 617.3128 1232.6110 1232.6091 1.60 0 28 0.046 +1Score > 42 indicates identity
Score > 28 indicates homology		 U X X K.GYRPQFYFR.T
1663 +3 411.8792 1232.6158 1232.6091 5.44 0 33 0.016 +1Score > 42 indicates identity
Score > 27 indicates homology		 U X X K.GYRPQFYFR.T
1675   413.5777 1237.7113 1237.7030 6.66 1 61 0.00014 +1Score > 35 indicates identity U X X R.VQDPLEIVGLR.D
1676 +4 619.8635 1237.7124 1237.7030 7.61 1 69 2.6e-005 +1Score > 35 indicates identity U X X R.VQDPLEIVGLR.D
2120   492.6282 1474.8628 1474.8548 5.41 0 41 0.0075 +1Score > 32 indicates identity U X X R.QVGVPYIVVFLNK.A
2123 +3 738.4399 1474.8652 1474.8548 7.09 0 69 1.2e-005 +1Score > 32 indicates identity U X X R.QVGVPYIVVFLNK.A
2259   766.9502 1531.8858 1531.8763 6.26 0 37 0.00049 +1Score > 33 indicates identity
Score > 17 indicates homology		 U X R.QVNVPYIVVFLNK.V
2342 +2 315.7942 1573.9346 1573.9304 2.67 0 25 0.16 +1Score > 30 indicates identity U X X R.GQVLVKPGSVKPHTK.F
2343   787.9747 1573.9348 1573.9304 2.81 0 72 3.5e-006 +1Score > 30 indicates identity U X X R.GQVLVKPGSVKPHTK.F
2343   787.9747 1573.9348 1573.9304 2.82 0 40 0.0052 +2Score > 30 indicates identity U X R.GQVLAKPGTIKPHTK.F
2344 +1 394.4910 1573.9349 1573.9304 2.84 0 37 0.00065 +1Score > 30 indicates identity
Score > 17 indicates homology		 U X X R.GQVLVKPGSVKPHTK.F
2344   394.4910 1573.9349 1573.9304 2.85 0 2 1.7 +2Score > 30 indicates identity
Score > 17 indicates homology		 U X R.GQVLAKPGTIKPHTK.F
2345 +1 525.6525 1573.9357 1573.9304 3.34 0 57 0.00011 +1Score > 30 indicates identity U X X R.GQVLVKPGSVKPHTK.F
2345 +1 525.6525 1573.9357 1573.9304 3.35 0 15 1.5 +2Score > 30 indicates identity U X R.GQVLAKPGTIKPHTK.F
2440 +4 807.9468 1613.8790 1613.8665 7.80 1 80 7.9e-007 +1Score > 38 indicates identity
Score > 31 indicates homology		 U X K.LVETLDAYIPEPVR.A
2441 +1 538.9677 1613.8813 1613.8665 9.18 1 38 0.0045 +1Score > 38 indicates identity
Score > 27 indicates homology		 U X K.LVETLDAYIPEPVR.A
2463 +2 815.9435 1629.8724 1629.8614 6.79 1 92 2e-007 +1Score > 39 indicates identity
Score > 38 indicates homology		 U X K.LVETLDSYIPEPVR.A
2465   544.3011 1629.8815 1629.8614 12.3 1 32 0.037 +1Score > 39 indicates identity
Score > 30 indicates homology		 U X K.LVETLDSYIPEPVR.A
2772   883.9711 1765.9276 1765.9224 2.98 0 83 2.1e-006 +1Score > 39 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T
2773 +2 354.1929 1765.9281 1765.9224 3.24 0 27 0.48 +1Score > 38 indicates identity
Score > 36 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T
2776 +1 442.4898 1765.9301 1765.9224 4.36 0 48 0.0061 +1Score > 38 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T
2777   589.6522 1765.9348 1765.9224 7.01 0 44 0.015 +1Score > 38 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T
2779 +2 442.7401 1766.9313 1766.9064 14.1 0 44 0.00045 +1Score > 39 indicates identity
Score > 23 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2780   589.9844 1766.9314 1766.9064 14.1 0 55 5.6e-005 +1Score > 39 indicates identity
Score > 25 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2781   354.3936 1766.9316 1766.9064 14.3 0 28 0.21 +1Score > 39 indicates identity
Score > 34 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2783   442.9528 1767.7821 1767.7787 1.89 0 8 0.37 +1Score > 39 indicates identity
Score > 16 indicates homology		 X X X X R.HYAHVDCPGHADYVK.N
2815   894.9700 1787.9254 1787.9166 4.95 1 86 1.1e-007 +1Score > 40 indicates identity
Score > 29 indicates homology		 U X R.GITINTAHVEYNSTIR.H
2820   597.3161 1788.9265 1788.9006 14.5 1 47 0.00031 +1Score > 40 indicates identity
Score > 25 indicates homology		 U X R.GITINTAHVEYNSTIR.H + Deamidated (NQ)
2839   601.3135 1800.9187 1800.9118 3.80 1 67 0.00011 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H
2840 +1 901.4675 1800.9204 1800.9118 4.78 1 106 1.5e-008 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H
2846 +1 601.6489 1801.9249 1801.8958 16.1 1 50 0.005 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H + Deamidated (NQ)
3408   712.3768 2134.1086 2134.0769 14.9 1 83 1.7e-006 +1Score > 38 indicates identity U X X R.AIDQPFLMPIEDVFSISGR.G
3410 +1 1068.5546 2135.0946 2135.0609 15.8 1 88 6.7e-008 +1Score > 39 indicates identity
Score > 29 indicates homology		 U X X R.AIDQPFLMPIEDVFSISGR.G + Deamidated (NQ)
3412   712.7077 2135.1013 2135.0609 18.9 1 83 1.4e-007 +1Score > 38 indicates identity
Score > 27 indicates homology		 U X X R.AIDQPFLMPIEDVFSISGR.G + Deamidated (NQ)
3451 +1 1076.5529 2151.0912 2151.0558 16.5 1 77 7e-007 +1Score > 39 indicates identity
Score > 28 indicates homology		 U X X R.AIDQPFLMPIEDVFSISGR.G + Deamidated (NQ); Oxidation (M)
3524 +1 1097.0646 2192.1146 2192.1551 -18.5 1 44 0.00039 +1Score > 39 indicates identity
Score > 23 indicates homology		 U X R.IIDKPFLMPIEDVFSISGR.G + Oxidation (M)
3525 +5 731.7125 2192.1157 2192.1551 -18.0 1 43 0.0005 +1Score > 39 indicates identity
Score > 23 indicates homology		 U X R.IIDKPFLMPIEDVFSISGR.G + Oxidation (M)

+45 subsets and intersections (638 subset proteins in total)

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 907 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
3 ATPA_RICAH 176 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
2 ATPA_VEREI 178 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 PORF_PSESY 690 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1
2 PORF_PSEAE 143 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+5

Accession Score Description
Family member distances as a dendrogram 1 RPOB_PSEPG 513 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
5 FTSZ_PSEPK 129 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOC_PSEP1 418 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoC PE=3 SV=1
3 RPOC_PSEU5 335 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas stutzeri (strain A1501) GN=rpoC PE=3 SV=1
6 RPOC_BIFLD 91 DNA-directed RNA polymerase subunit beta' OS=Bifidobacterium longum (strain DJO10A) GN=rpoC PE=3 SV=1
4 RPOC_SYNAS 202 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1

+6

Accession Score Description
1 RS5_PSEE4 428 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+7

Accession Score Description
1 RL3_PSEP1 378 50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1

+8

Accession Score Description
1 ARPC_PSEPU 369 Antibiotic efflux pump outer membrane protein arpC OS=Pseudomonas putida GN=arpC PE=2 SV=1

+9

Accession Score Description
1 RL2_PSEP1 329 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1

+10

Accession Score Description
Family member distances as a dendrogram 1 DLDH2_PSEPU 325 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4
2 DLDH_AZOVI 161 Dihydrolipoyl dehydrogenase OS=Azotobacter vinelandii PE=1 SV=1
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