MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 191–200 (out of 230)


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+191

Accession Score Description
1 SYR_EUBR3 30 Arginyl-tRNA synthetase OS=Eubacterium rectale (strain ATCC 33656 / VPI 0990) GN=argS PE=3 SV=1

+192

Accession Score Description
1 ARCC_PSEAE 30 Carbamate kinase OS=Pseudomonas aeruginosa GN=arcC PE=3 SV=1

-193

Accession Score Description
1 ODO2_BUCAP 30 description
Score Mass Matches Sequences emPAI
193.1 ODO2_BUCAP 30 0 2 (1) 2 (1) 0.05
description
+90 samesets of ODO2_BUCAP

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
252   545.3285 544.3212 544.3221 -1.52 0 20 0.69 +1Score > 43 indicates identity
Score > 31 indicates homology
R.LIDGK.E
462   348.1724 694.3302 694.3286 2.42 1 30 0.052 +1Score > 41 indicates identity
Score > 30 indicates homology
U K.IYENR.I + Deamidated (NQ)

+194

Accession Score Description
1 MURC_MYCBO 30 UDP-N-acetylmuramate--L-alanine ligase OS=Mycobacterium bovis GN=murC PE=3 SV=1

+195

Accession Score Description
1 BIOD_ALTMD 30 Dethiobiotin synthetase OS=Alteromonas macleodii (strain DSM 17117 / Deep ecotype) GN=bioD PE=3 SV=1

+196

Accession Score Description
1 OTC_YEAST 29 Ornithine carbamoyltransferase OS=Saccharomyces cerevisiae GN=ARG3 PE=1 SV=1

+197

Accession Score Description
1 ALKD_PSEPU 29 2-dehydro-3-deoxy-phosphogluconate aldolase OS=Pseudomonas putida GN=eda PE=1 SV=2

+198

Accession Score Description
1 MQO1_PSEPK 29 Probable malate:quinone oxidoreductase 1 OS=Pseudomonas putida (strain KT2440) GN=mqo1 PE=3 SV=1

+199

Accession Score Description
1 OXAA_RUTMC 29 Inner membrane protein oxaA OS=Ruthia magnifica subsp. Calyptogena magnifica GN=oxaA PE=3 SV=1

+200

Accession Score Description
1 RPOB_SULAC 29 DNA-directed RNA polymerase subunit B OS=Sulfolobus acidocaldarius GN=rpoB PE=3 SV=2
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