MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 230)

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+11

Accession Score Description
1 ODB2_PSEPU 324 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+12

Accession Score Description
1 DHSA_ECOLI 318 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+13

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEE4 298 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1
2 RL4_PSE14 297 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+14

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 297 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
2 CH601_ECOK1 101 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
3 CH60_GEOLS 90 60 kDa chaperonin OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=groL PE=3 SV=1

+15

Accession Score Description
1 RS2_PSEP1 297 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

+16

Accession Score Description
1 ODO2_PSEAE 292 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+17

Accession Score Description
1 SUCC_PSEPK 257 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+18

Accession Score Description
1 EFG1_PSEPK 240 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

-19

Accession Score Description
1 ARPA_PSEPU 230 Antibiotic efflux pump periplasmic linker protein arpA OS=Pseudomonas putida GN=arpA PE=2 SV=1
Score Mass Matches Sequences emPAI
19.1 ARPA_PSEPU 230 40366 11 (6) 10 (5) 0.39
Antibiotic efflux pump periplasmic linker protein arpA OS=Pseudomonas putida GN=arpA PE=2 SV=1
1 sameset of ARPA_PSEPU
TTGA_PSEPU 230 41281 11 (6) 10 (5) 0.38
Toluene efflux pump periplasmic linker protein ttgA OS=Pseudomonas putida GN=ttgA PE=2 SV=1

-11 peptide matches (11 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
584   379.7310 757.4474 757.4446 3.75 0 26 0.15 +3Score > 43 indicates identity
Score > 30 indicates homology		 U K.ANLLATR.S
701   406.7540 811.4934 811.4916 2.31 0 47 0.0045 +1Score > 36 indicates identity U K.VLAPISGR.I
1186   509.2655 1016.5164 1016.5138 2.58 1 24 0.45 +1Score > 42 indicates identity
Score > 33 indicates homology		 U R.DLESGQLQK.A
1426   566.2876 1130.5606 1130.5819 -18.8 1 24 1.7 +2Score > 42 indicates identity
Score > 39 indicates homology		 U K.QLIDEQAVSK.Q + Deamidated (NQ)
1851   670.8647 1339.7148 1339.7096 3.94 1 61 4.5e-005 +1Score > 39 indicates identity
Score > 30 indicates homology		 U K.GAPTALVVNQENK.V
2266   512.9841 1535.9305 1535.9035 17.6 1 7 1.1 +2Score > 29 indicates identity
Score > 20 indicates homology		 U R.VAEVRPQVNGIILK.R + Deamidated (NQ)
2597   840.4648 1678.9150 1678.9115 2.14 0 68 5e-006 +1Score > 37 indicates identity
Score > 27 indicates homology		 U K.AGVNANAILAPQQGVTR.D
3060   645.3377 1932.9913 1932.9792 6.22 1 58 0.00071 +1Score > 39 indicates identity U K.AGNNAASVQLVLEDGSLFK.Q + Deamidated (NQ)
3061   967.5037 1932.9928 1932.9792 7.04 1 98 3.4e-009 +1Score > 39 indicates identity
Score > 26 indicates homology		 U K.AGNNAASVQLVLEDGSLFK.Q + Deamidated (NQ)
3406   534.2875 2133.1209 2133.0830 17.8 0 48 0.00017 +1Score > 38 indicates identity
Score > 23 indicates homology		 U R.ALFPNPDHTLLPGMFVHAR.L + Deamidated (NQ)
3531   1097.5460 2193.0774 2193.0953 -8.15 1 5 7.1 +4Score > 39 indicates identity
Score > 26 indicates homology		 U E.GQQLYQIDPAVYEATLANAK.A + Deamidated (NQ)

1 subset or intersection (2 subset proteins in total)

Score Mass Subset of
MEPA_PSEPU 220 41282 19.1
Multidrug/solvent efflux pump periplasmic linker protein mepA OS=Pseudomonas putida GN=mepA PE=1 SV=1
1 sameset of MEPA_PSEPU
TTGA_PSEPK 220 41282
Probable efflux pump periplasmic linker ttgA OS=Pseudomonas putida (strain KT2440) GN=ttgA PE=1 SV=1

+20

Accession Score Description
1 RL18_PSEP1 226 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
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