MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 181–190 (out of 230)


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-181

Accession Score Description
1 DNLJ_THETN 33 DNA ligase OS=Thermoanaerobacter tengcongensis GN=ligA PE=3 SV=1
Score Mass Matches Sequences emPAI
181.1 DNLJ_THETN 33 75202 2 (1) 1 (1) 0.03
DNA ligase OS=Thermoanaerobacter tengcongensis GN=ligA PE=3 SV=1

+2 peptide matches (1 non-duplicate, 1 duplicate)


+182

Accession Score Description
1 CARA_PSEPK 33 Carbamoyl-phosphate synthase small chain OS=Pseudomonas putida (strain KT2440) GN=carA PE=3 SV=1

+183

Accession Score Description
1 PHS_PSEAB 32 Putative pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=PA14_53000 PE=3 SV=1

+184

Accession Score Description
1 PTPC1_DANRE 32 Protein tyrosine phosphatase domain-containing protein 1 OS=Danio rerio GN=ptpdc1 PE=2 SV=1

+185

Accession Score Description
1 PNP_PSEPK 32 Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1

+186

Accession Score Description
1 LPTD_PSEPW 32 LPS-assembly protein lptD OS=Pseudomonas putida (strain W619) GN=lptD PE=3 SV=1

+187

Accession Score Description
1 RLX_META3 32 50S ribosomal protein LX OS=Methanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280) GN=rplX PE=3 SV=1

+188

Accession Score Description
1 NDC80_MOUSE 31 Kinetochore protein NDC80 homolog OS=Mus musculus GN=Ndc80 PE=2 SV=1

+189

Accession Score Description
1 ACCC_ECOLI 31 Biotin carboxylase OS=Escherichia coli (strain K12) GN=accC PE=1 SV=2

+190

Accession Score Description
1 Y084_ATV 31 Uncharacterized protein ORF84 OS=Acidianus two-tailed virus PE=4 SV=1
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