MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 161–170 (out of 230)

Page: Previous 1 …12 13 14 15 16 17 18 19 20 21 22 … 23 Next 

+161

Accession Score Description
1 CBPM_PSEPK 38 Chaperone modulatory protein cbpM OS=Pseudomonas putida (strain KT2440) GN=cbpM PE=3 SV=1

+162

Accession Score Description
1 RRP3_CANGA 38 ATP-dependent rRNA helicase RRP3 OS=Candida glabrata GN=RRP3 PE=3 SV=1

+163

Accession Score Description
1 FKBY_HAEIN 38 Probable FKBP-type peptidyl-prolyl cis-trans isomerase OS=Haemophilus influenzae GN=HI0574 PE=1 SV=1

+164

Accession Score Description
1 RAPG_BACSU 37 Response regulator aspartate phosphatase G OS=Bacillus subtilis GN=rapG PE=2 SV=1

+165

Accession Score Description
1 XYLB_ECOLI 37 Xylulose kinase OS=Escherichia coli (strain K12) GN=xylB PE=1 SV=1

+166

Accession Score Description
1 EFP_PSEPG 37 Elongation factor P OS=Pseudomonas putida (strain GB-1) GN=efp PE=3 SV=1

+167

Accession Score Description
1 PIFA_ECOLI 37 Phage T7 exclusion protein OS=Escherichia coli (strain K12) GN=pifA PE=4 SV=2

+168

Accession Score Description
1 RL15_PSEE4 37 50S ribosomal protein L15 OS=Pseudomonas entomophila (strain L48) GN=rplO PE=3 SV=1

+169

Accession Score Description
1 HEX_VIBVU 36 Beta-hexosaminidase OS=Vibrio vulnificus GN=hex PE=3 SV=1

-170

Accession Score Description
1 RS1_PSEAE 36 30S ribosomal protein S1 OS=Pseudomonas aeruginosa GN=rpsA PE=3 SV=1
Score Mass Matches Sequences emPAI
170.1 RS1_PSEAE 36 61946 9 (3) 7 (1) 0.04
30S ribosomal protein S1 OS=Pseudomonas aeruginosa GN=rpsA PE=3 SV=1
7 samesets of RS1_PSEAE
RS1_BUCAI 36 62849 7 (3) 5 (1) 0.04
30S ribosomal protein S1 OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=rpsA PE=3 SV=1
RS1_BUCAP 36 62605 7 (3) 5 (1) 0.04
30S ribosomal protein S1 OS=Buchnera aphidicola subsp. Schizaphis graminum GN=rpsA PE=3 SV=1
RS1_ECO57 36 61235 7 (3) 5 (1) 0.04
30S ribosomal protein S1 OS=Escherichia coli O157:H7 GN=rpsA PE=3 SV=1
RS1_ECOL6 36 61235 7 (3) 5 (1) 0.04
30S ribosomal protein S1 OS=Escherichia coli O6 GN=rpsA PE=3 SV=1
RS1_ECOLI 36 61235 7 (3) 5 (1) 0.04
30S ribosomal protein S1 OS=Escherichia coli (strain K12) GN=rpsA PE=1 SV=1
RS1_PROSP 36 41702 7 (3) 5 (1) 0.06
30S ribosomal protein S1 (Fragment) OS=Providencia sp. GN=rpsA PE=3 SV=1
RS1_SHIFL 36 61235 7 (3) 5 (1) 0.04
30S ribosomal protein S1 OS=Shigella flexneri GN=rpsA PE=3 SV=1

-9 peptide matches (7 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
287   587.3529 586.3456 586.3439 3.01 0 15 0.39 +1Score > 44 indicates identity
Score > 23 indicates homology		 U K.GVINGK.V
323   308.7061 615.3976 615.3956 3.40 0 19 0.51 +1Score > 42 indicates identity
Score > 29 indicates homology		 R.VSLGLK.Q
349   315.7139 629.4132 629.4112 3.26 0 27 0.49 +1Score > 38 indicates identity
Score > 36 indicates homology		 R.ISLGIK.Q
648   394.2258 786.4370 786.4348 2.86 0 25 0.59 +1Score > 44 indicates identity
Score > 35 indicates homology		 R.NNVVVSR.R
1435   567.8008 1133.5870 1133.5829 3.62 0 28 0.21 +1Score > 42 indicates identity
Score > 34 indicates homology		 U K.GGFTVDVNGIR.A
2246 +2 509.3059 1524.8959 1524.8777 11.9 0 26 0.01 +1Score > 33 indicates identity
Score > 19 indicates homology		 U R.AFLPGSLVDVRPVR.D
4209   946.4799 2836.4179 2836.3742 15.4 0 6 1 +1Score > 37 indicates identity
Score > 18 indicates homology		 K.NLTDYGAFVDLGGVDGLLHITDMAWK.R + Oxidation (M)
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