MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 sp |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 22:50:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODB2_PSEPU | 324 | Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSA_ECOLI | 318 | Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL4_PSEE4 | 298 | 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1 | |
| 2 | RL4_PSE14 | 297 | 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1 | |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_PSEPK | 297 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 | |
| 2 | CH601_ECOK1 | 101 | 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1 | |
| 3 | CH60_GEOLS | 90 | 60 kDa chaperonin OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=groL PE=3 SV=1 | |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS2_PSEP1 | 297 | 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODO2_PSEAE | 292 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCC_PSEPK | 257 | Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 17.1 | SUCC_PSEPK |
257 | 41499 | 13 (9) | 12 (8) | 0.54 | |
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1 | |||||||
| 3 samesets of SUCC_PSEPK | |||||||
| SUCC_PSEP1 |
257 | 41499 | 13 (9) | 12 (8) | 0.54 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=sucC PE=3 SV=1 | |||||||
| SUCC_PSEPW |
257 | 41472 | 12 (9) | 11 (8) | 0.54 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain W619) GN=sucC PE=3 SV=1 | |||||||
| SUCC_PSEE4 |
257 | 41472 | 12 (9) | 11 (8) | 0.54 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas entomophila (strain L48) GN=sucC PE=3 SV=1 | |||||||
13 peptide matches (12 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 176 |
431.2636 | 430.2563 | 430.2540 | 5.44 | 0 | 37 | 0.11 | 1Score > 40 indicates identity Score > 40 indicates homology |
K.AGGVK.L | ||
| 396 |
334.7272 | 667.4398 | 667.4381 | 2.60 | 0 | 31 | 0.032 | 1Score > 29 indicates identity |
U | K.VPVVVR.L | |
| 567 |
374.2431 | 746.4716 | 746.4691 | 3.47 | 0 | 34 | 0.041 | 1Score > 33 indicates identity |
U | K.IFVGLAK.L | |
| 1127 |
496.2735 | 990.5324 | 990.5287 | 3.80 | 0 | 36 | 0.0043 | 1Score > 41 indicates identity Score > 25 indicates homology |
U | K.AFAAQWLGK.N | |
| 1153 |
501.2767 | 1000.5388 | 1000.5342 | 4.67 | 1 | 30 | 0.21 | 1Score > 42 indicates identity Score > 36 indicates homology |
U | K.IGGNEWVVK.A | |
| 1439 |
567.8148 | 1133.6150 | 1133.6081 | 6.16 | 1 | 14 | 0.5 | 1Score > 40 indicates identity Score > 24 indicates homology |
K.ELYLGAVVDR.S | ||
| 1762 |
430.1913 | 1287.5521 | 1287.5480 | 3.16 | 0 | 21 | 0.1 | 1Score > 37 indicates identity Score > 23 indicates homology |
U | K.TFHDPSQDDAR.E | |
| 1870 |
676.3734 | 1350.7322 | 1350.7183 | 10.3 | 1 | 73 | 1.8e-006 | 1Score > 38 indicates identity Score > 28 indicates homology |
U | K.QLFAEYGLPVSK.G | |
| 2329 |
785.4303 | 1568.8460 | 1568.8311 | 9.53 | 0 | 67 | 8.5e-006 | 1Score > 38 indicates identity Score > 29 indicates homology |
U | K.ATIDPLVGAQPFQGR.E | |
| 2608 |
563.9954 | 1688.9644 | 1688.9349 | 17.5 | 1 | 36 | 0.00098 | 1Score > 34 indicates identity Score > 18 indicates homology |
U | K.DHDLALLEVNPLVIK.A + Deamidated (NQ) | |
| 2720 |
868.4416 | 1734.8686 | 1734.8424 | 15.1 | 0 | 67 | 4.2e-006 | 1Score > 40 indicates identity Score > 26 indicates homology |
U | K.NLVTYQTDANGQPVSK.I + Deamidated (NQ) | |
| 3842 |
1 |
805.1207 | 2412.3403 | 2412.3111 | 12.1 | 1 | 65 | 8.8e-007 | 1Score > 32 indicates identity Score > 17 indicates homology |
U | K.VLAESGLNIIAATSLTDAAQQVVK.A + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| SUCC_PSEPG |
198 | 41413 | 17.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain GB-1) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEA7 |
187 | 41772 | 17.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain PA7) GN=sucC PE=3 SV=1 | |||||
| 3 samesets of SUCC_PSEA7 | |||||
| SUCC_PSEAB |
187 | 41830 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEAE |
187 | 41802 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa GN=sucC PE=3 SV=2 | |||||
| SUCC_PSEA8 |
187 | 41802 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain LESB58) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEF5 |
184 | 41599 | 17.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=sucC PE=3 SV=1 | |||||
| 1 sameset of SUCC_PSEF5 | |||||
| SUCC_PSEFS |
184 | 41547 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain SBW25) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSE14 |
181 | 41494 | 17.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=sucC PE=3 SV=1 | |||||
| 2 samesets of SUCC_PSE14 | |||||
| SUCC_PSESM |
181 | 41472 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. tomato GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEU2 |
181 | 41442 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. syringae (strain B728a) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEPF |
155 | 41527 | 17.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain Pf0-1) GN=sucC PE=3 SV=1 | |||||
| SUCC_MARAV |
76 | 41495 | 17.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=sucC PE=3 SV=1 | |||||
| 2 samesets of SUCC_MARAV | |||||
| SUCC_PSEU5 |
76 | 41761 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas stutzeri (strain A1501) GN=sucC PE=3 SV=1 | |||||
| SUCC_SACD2 |
76 | 41524 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEMY |
73 | 41759 | 17.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas mendocina (strain ymp) GN=sucC PE=3 SV=1 | |||||
| 1 sameset of SUCC_PSEMY | |||||
| SUCC_TERTT |
73 | 41703 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=sucC PE=3 SV=1 | |||||
| SUCC_VIBC3 |
67 | 41658 | 17.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=sucC PE=3 SV=1 | |||||
| 2 samesets of SUCC_VIBC3 | |||||
| SUCC_VIBCH |
67 | 41658 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae GN=sucC PE=3 SV=1 | |||||
| SUCC_VIBCM |
67 | 41658 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae serotype O1 (strain M66-2) GN=sucC PE=3 SV=1 | |||||
| SUCC_ALCBS |
34 | 41662 | 17.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=sucC PE=3 SV=1 | |||||
| PARB2_DEIRA |
31 | 32872 | 17.1 | ||
| Probable chromosome 2-partitioning protein parB OS=Deinococcus radiodurans GN=parB2 PE=3 SV=1 | |||||
| 46 samesets of PARB2_DEIRA |
|||||
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFG1_PSEPK | 240 | Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARPA_PSEPU | 230 | Antibiotic efflux pump periplasmic linker protein arpA OS=Pseudomonas putida GN=arpA PE=2 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL18_PSEP1 | 226 | 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1 |
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