MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 230)


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+11

Accession Score Description
1 ODB2_PSEPU 324 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+12

Accession Score Description
1 DHSA_ECOLI 318 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+13

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEE4 298 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1
2 RL4_PSE14 297 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+14

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 297 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
2 CH601_ECOK1 101 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
3 CH60_GEOLS 90 60 kDa chaperonin OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=groL PE=3 SV=1

+15

Accession Score Description
1 RS2_PSEP1 297 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

-16

Accession Score Description
1 ODO2_PSEAE 292 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1
Score Mass Matches Sequences emPAI
16.1 ODO2_PSEAE 292 42861 18 (12) 7 (3) 0.30
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

-18 peptide matches (10 non-duplicate, 8 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
252   545.3285 544.3212 544.3221 -1.52 0 20 0.69 +1Score > 43 indicates identity
Score > 31 indicates homology
R.LIDGK.E
269   573.3618 572.3545 572.3533 2.08 1 34 0.51 +1Score > 43 indicates identity M.AIEIK.A
375   326.1716 650.3286 650.3275 1.72 1 21 0.61 +1Score > 43 indicates identity
Score > 31 indicates homology
K.DLFEK.K
376   651.3369 650.3296 650.3275 3.22 1 25 0.86 +1Score > 43 indicates identity
Score > 37 indicates homology
K.DLFEK.K
480   703.4034 702.3961 702.3912 7.05 1 41 0.091 +1Score > 43 indicates identity K.AATEALK.R
798 +3 426.7901 851.5656 851.5593 7.48 0 41 0.0011 +1Score > 24 indicates identity U R.GLVVPVLR.N
799 +1 852.5732 851.5659 851.5593 7.80 0 41 0.0011 +1Score > 24 indicates identity U R.GLVVPVLR.N
1300 +3 538.2888 1074.5630 1074.5572 5.41 0 59 0.00018 +1Score > 42 indicates identity
Score > 34 indicates homology
U R.LGFMSFFVK.A
1304 +1 1075.5811 1074.5738 1074.5572 15.4 0 49 0.00054 +1Score > 41 indicates identity
Score > 29 indicates homology
U R.LGFMSFFVK.A
3032   957.4798 1912.9450 1912.9319 6.85 1 101 1.2e-009 +1Score > 40 indicates identity
Score > 24 indicates homology
U K.APTFPESVADGTVATWHK.K

3 subsets and intersections (14 subset proteins in total)

Score Mass Subset of
ODO2_AZOVI 215 41976 16.1
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Azotobacter vinelandii GN=sucB PE=1 SV=2
ODO2_BUCAI 142 48175 16.1
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=sucB PE=3 SV=1
5 samesets of ODO2_BUCAI
ODO2_RICCN 142 42829
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Rickettsia conorii GN=sucB PE=3 SV=1
ODO2_RICFE 142 43491
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Rickettsia felis GN=sucB PE=3 SV=1
ODO2_RICPR 142 44123
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Rickettsia prowazekii GN=sucB PE=3 SV=1
ODO2_RICTY 142 43853
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Rickettsia typhi GN=sucB PE=3 SV=1
ODO2_RALEH 142 43223
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) GN=sucB PE=3 SV=1
ODO2_BOVIN 99 49284 16.1
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial OS=Bos taurus GN=DLST PE=1 SV=2
6 samesets of ODO2_BOVIN
ODO2_HUMAN 99 49041
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial OS=Homo sapiens GN=DLST PE=1 SV=3
ODO2_MOUSE 99 49306
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial OS=Mus musculus GN=Dlst PE=1 SV=1
ODO2_RAT 99 49236
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial OS=Rattus norvegicus GN=Dlst PE=1 SV=2
ODO2_PIG 99 49288
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial OS=Sus scrofa GN=DLST PE=1 SV=1
ODO2_DICDI 99 47775
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial OS=Dictyostelium discoideum GN=odhB PE=1 SV=1
ODO2_TAKRU 99 44255
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial (Fragment) OS=Takifugu rubripes GN=dlst PE=3 SV=1

+17

Accession Score Description
1 SUCC_PSEPK 257 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+18

Accession Score Description
1 EFG1_PSEPK 240 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+19

Accession Score Description
1 ARPA_PSEPU 230 Antibiotic efflux pump periplasmic linker protein arpA OS=Pseudomonas putida GN=arpA PE=2 SV=1

+20

Accession Score Description
1 RL18_PSEP1 226 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
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