MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 230)

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+11

Accession Score Description
1 ODB2_PSEPU 324 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+12

Accession Score Description
1 DHSA_ECOLI 318 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+13

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEE4 298 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1
2 RL4_PSE14 297 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+14

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 297 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
2 CH601_ECOK1 101 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
3 CH60_GEOLS 90 60 kDa chaperonin OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=groL PE=3 SV=1

-15

Accession Score Description
1 RS2_PSEP1 297 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1
Score Mass Matches Sequences emPAI
15.1 RS2_PSEP1 297 27063 31 (19) 11 (7) 2.16
30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1
1 sameset of RS2_PSEP1
RS2_PSEPK 297 27063 31 (19) 11 (7) 2.16
30S ribosomal protein S2 OS=Pseudomonas putida (strain KT2440) GN=rpsB PE=3 SV=1

-31 peptide matches (23 non-duplicate, 8 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
487   354.1801 706.3456 706.3438 2.56 0 15 0.81 +1Score > 41 indicates identity
Score > 27 indicates homology		 R.YWNPK.M
488   707.3535 706.3462 706.3438 3.37 0 28 0.14 +1Score > 40 indicates identity
Score > 32 indicates homology		 R.YWNPK.M
520   363.7015 725.3884 725.3860 3.31 0 14 1.2 +3Score > 37 indicates identity
Score > 28 indicates homology		 K.YIFGAR.N
521   726.3965 725.3892 725.3860 4.38 0 21 0.54 +1Score > 37 indicates identity
Score > 31 indicates homology		 K.YIFGAR.N
542   367.6849 733.3552 733.3541 1.57 0 10 4.7 +8Score > 43 indicates identity
Score > 29 indicates homology		 U M.SQVNMR.D
628 +3 389.2484 776.4822 776.4796 3.37 0 43 0.0054 +1Score > 33 indicates identity U K.ILFVGTK.R
630   777.4896 776.4823 776.4796 3.47 0 53 0.00053 +1Score > 33 indicates identity U K.ILFVGTK.R
815 +1 429.2466 856.4786 856.4766 2.35 1 41 0.051 +1Score > 40 indicates identity U K.IVAEQAAR.C
816   857.4860 856.4787 856.4766 2.43 1 64 0.00027 +1Score > 40 indicates identity U K.IVAEQAAR.C
821   430.2484 858.4822 858.4810 1.40 1 42 0.048 +1Score > 42 indicates identity
Score > 42 indicates homology		 U R.IAITEANK.L
822   859.4901 858.4828 858.4810 2.07 1 59 0.0014 +1Score > 43 indicates identity U R.IAITEANK.L
1086 +2 322.5318 964.5736 964.5705 3.14 1 27 0.24 +1Score > 34 indicates identity
Score > 34 indicates homology		 U K.IHIVNLEK.T
1087   483.2946 964.5746 964.5705 4.26 1 18 0.24 +1Score > 34 indicates identity
Score > 24 indicates homology		 U K.IHIVNLEK.T
1372   370.5255 1108.5547 1108.5526 1.83 0 38 0.0074 +1Score > 41 indicates identity
Score > 29 indicates homology		 U K.AGVHFGHQTR.Y
1373   555.2849 1108.5552 1108.5526 2.35 0 18 0.16 +1Score > 41 indicates identity
Score > 22 indicates homology		 U K.AGVHFGHQTR.Y
1556   591.8077 1181.6008 1181.5903 8.93 0 31 0.013 +1Score > 40 indicates identity
Score > 25 indicates homology		 U R.WLGGMLTNYK.T
2491   547.2792 1638.8158 1638.8076 5.00 1 82 3.9e-006 +1Score > 40 indicates identity U K.TLPMFNDALSFVER.L
2492 +1 820.4160 1638.8174 1638.8076 6.03 1 62 2.6e-005 +1Score > 40 indicates identity
Score > 28 indicates homology		 U K.TLPMFNDALSFVER.L
2495   820.9069 1639.7992 1639.7916 4.68 1 17 0.054 +1Score > 40 indicates identity
Score > 17 indicates homology		 U K.TLPMFNDALSFVER.L + Deamidated (NQ)
2532   828.4158 1654.8170 1654.8025 8.80 1 25 0.013 +1Score > 40 indicates identity
Score > 18 indicates homology		 U K.TLPMFNDALSFVER.L + Oxidation (M)
4400 +1 1032.8754 3095.6044 3095.5663 12.3 1 68 2.9e-005 +1Score > 35 indicates identity U K.LGIPVIGVVDTNSSPEGVDYIIPGNDDAIR.A + Deamidated (NQ)
4401   774.9089 3095.6065 3095.5663 13.0 1 37 0.033 +1Score > 35 indicates identity U K.LGIPVIGVVDTNSSPEGVDYIIPGNDDAIR.A + Deamidated (NQ)
4402   1032.8773 3095.6101 3095.5663 14.1 1 44 0.0057 +1Score > 35 indicates identity U K.LGIPVIGVVDTNSSPEGVDYIIPGNDDAIR.A + Deamidated (NQ)

+19 subsets and intersections (401 subset proteins in total)

+16

Accession Score Description
1 ODO2_PSEAE 292 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+17

Accession Score Description
1 SUCC_PSEPK 257 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+18

Accession Score Description
1 EFG1_PSEPK 240 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+19

Accession Score Description
1 ARPA_PSEPU 230 Antibiotic efflux pump periplasmic linker protein arpA OS=Pseudomonas putida GN=arpA PE=2 SV=1

+20

Accession Score Description
1 RL18_PSEP1 226 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
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