MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 141–150 (out of 230)


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+141

Accession Score Description
1 SYGB_SHEHH 44 Glycyl-tRNA synthetase beta subunit OS=Shewanella halifaxensis (strain HAW-EB4) GN=glyS PE=3 SV=1

+142

Accession Score Description
1 CDC48_YEAST 43 Cell division control protein 48 OS=Saccharomyces cerevisiae GN=CDC48 PE=1 SV=3

+143

Accession Score Description
1 DNAK_KLEP3 43 Chaperone protein dnaK OS=Klebsiella pneumoniae (strain 342) GN=dnaK PE=2 SV=1

+144

Accession Score Description
1 ARGJ_OCEIH 43 Arginine biosynthesis bifunctional protein argJ OS=Oceanobacillus iheyensis GN=argJ PE=3 SV=1

+145

Accession Score Description
1 RAVA_ECOBW 42 ATPase ravA OS=Escherichia coli (strain K12 / BW2952) GN=ravA PE=3 SV=1

+146

Accession Score Description
1 RL24_PSEE4 42 50S ribosomal protein L24 OS=Pseudomonas entomophila (strain L48) GN=rplX PE=3 SV=1

+147

Accession Score Description
1 CAPB_PSEFR 42 Cold shock protein capB OS=Pseudomonas fragi GN=capB PE=1 SV=1

+148

Accession Score Description
1 FMT_STRPI 42 Methionyl-tRNA formyltransferase OS=Streptococcus pneumoniae (strain Hungary19A-6) GN=fmt PE=3 SV=1

-149

Accession Score Description
1 EFR3_CHAGB 42 Protein EFR3 OS=Chaetomium globosum GN=EFR3 PE=3 SV=1
Score Mass Matches Sequences emPAI
149.1 EFR3_CHAGB 42 130046 2 (1) 2 (1) 0.02
Protein EFR3 OS=Chaetomium globosum GN=EFR3 PE=3 SV=1
1 sameset of EFR3_CHAGB
Y2850_MYCTU 42 67117 2 (1) 2 (1) 0.03
Uncharacterized protein Rv2850c/MT2916 OS=Mycobacterium tuberculosis GN=Rv2850c PE=3 SV=1

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
466   350.2299 698.4452 698.4439 1.94 0 42 0.021 +1Score > 37 indicates identity U K.LGGALLR.R
728   827.5371 826.5298 826.5388 -10.9 1 30 0.29 +7Score > 37 indicates identity U E.KLGGALLR.R

+150

Accession Score Description
1 LEU3_CHRVO 41 3-isopropylmalate dehydrogenase OS=Chromobacterium violaceum GN=leuB PE=3 SV=1
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