MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 230)

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+11

Accession Score Description
1 ODB2_PSEPU 324 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+12

Accession Score Description
1 DHSA_ECOLI 318 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+13

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEE4 298 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1
2 RL4_PSE14 297 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

-14

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 297 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
2 CH601_ECOK1 101 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
3 CH60_GEOLS 90 60 kDa chaperonin OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=groL PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
CH60_PSEPK 297 56765 29 (15) 20 (11) 0.67
60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
CH601_ECOK1 101 57464 18 (5) 9 (4) 0.22
60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
+27 samesets of CH601_ECOK1
CH60_GEOLS 90 58655 7 (4) 5 (3) 0.17
60 kDa chaperonin OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=groL PE=3 SV=1

-38 peptide matches (31 non-duplicate, 7 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
212   487.3255 486.3182 486.3166 3.39 0 18 1.9 +6Score > 46 indicates identity
Score > 34 indicates homology		 X X K.VAAVK.A
277   586.3574 585.3501 585.3486 2.60 0 36 0.12 +1Score > 39 indicates identity U X K.ATLGPK.G
301   301.6854 601.3562 601.3547 2.51 0 22 1.4 +4Score > 43 indicates identity
Score > 35 indicates homology		 X X K.ISNIR.E
302   602.3646 601.3573 601.3547 4.30 0 22 1 +3Score > 43 indicates identity
Score > 34 indicates homology		 X X K.ISNIR.E
361   643.4159 642.4086 642.4064 3.39 0 40 0.081 +1Score > 41 indicates identity U X R.NVVLAK.S
365   322.7088 643.4030 643.4017 2.11 1 9 1.7 +8Score > 44 indicates identity
Score > 24 indicates homology		 X X R.VKQIR.A + Deamidated (NQ)
377   326.6575 651.3004 651.2976 4.31 0 16 0.67 +1Score > 40 indicates identity
Score > 27 indicates homology		 X K.FGDSAR.K
421   338.1859 674.3572 674.3599 -3.95 0 28 0.76 +1Score > 43 indicates identity
Score > 39 indicates homology		 U X X K.DGVSVAK.E
422   675.3698 674.3625 674.3599 3.88 0 44 0.04 +1Score > 43 indicates identity U X X K.DGVSVAK.E
508   360.1780 718.3414 718.3398 2.23 0 29 0.49 +1Score > 43 indicates identity
Score > 38 indicates homology		 X X X K.APGFGDR.R
609   386.2227 770.4308 770.4286 2.87 1 26 0.61 +1Score > 39 indicates identity
Score > 37 indicates homology		 U X R.AVESPLR.Q
728   827.5371 826.5298 826.5276 2.65 0 42 0.02 +1Score > 37 indicates identity U X X K.LAGGVAVIK.V
730   414.2726 826.5306 826.5276 3.65 0 19 0.92 +1Score > 36 indicates identity
Score > 31 indicates homology		 U X X K.LAGGVAVIK.V
958   921.5068 920.4995 920.4967 3.03 0 52 0.0039 +1Score > 40 indicates identity U X X K.SFGAPTITK.D
959   461.2576 920.5006 920.4967 4.25 0 33 0.14 +1Score > 40 indicates identity
Score > 37 indicates homology		 U X X K.SFGAPTITK.D
975 +1 464.3014 926.5882 926.5800 8.87 0 54 0.00054 +1Score > 34 indicates identity U X R.ALAAIIDLK.G
1096 +1 486.7919 971.5692 971.5651 4.24 1 55 0.0017 +1Score > 40 indicates identity U X K.ATAAVVAELK.N
1173   337.8465 1010.5177 1010.5145 3.14 1 43 0.0026 +1Score > 40 indicates identity
Score > 29 indicates homology		 U X X X R.VEDALHATR.A
1174   506.2665 1010.5184 1010.5145 3.91 1 63 0.00023 +1Score > 40 indicates identity U X X X R.VEDALHATR.A
1827   661.8278 1321.6410 1321.6336 5.62 1 35 0.012 +1Score > 41 indicates identity
Score > 28 indicates homology		 U X K.DAFENMGAQLVK.E
1836   664.8887 1327.7628 1327.7534 7.15 0 57 0.0002 +1Score > 37 indicates identity
Score > 32 indicates homology		 U X K.MLVGVNVLADAVK.A
1944   693.3956 1384.7766 1384.7860 -6.78 1 3 1.9 +5Score > 38 indicates identity
Score > 19 indicates homology		 U X K.MLRGVNVLADAVK.V
2009   707.3121 1412.6096 1412.6056 2.89 1 52 0.0022 +1Score > 38 indicates identity U X R.AQIEDTTSDYDR.E
2223   757.3872 1512.7598 1512.7532 4.38 1 76 1.1e-005 +1Score > 40 indicates identity
Score > 39 indicates homology		 U X K.GDNEDQNVGIALLR.R
2224   505.2607 1512.7603 1512.7532 4.66 1 52 0.0026 +1Score > 40 indicates identity
Score > 38 indicates homology		 U X K.GDNEDQNVGIALLR.R
2354 +2 790.9595 1579.9044 1579.9297 -16.0 1 16 3.4 +2Score > 34 indicates identity X R.GVNVLADAVKVTLGPK.G
2355   527.6426 1579.9060 1579.9297 -15.0 1 7 7.1 +7Score > 34 indicates identity
Score > 28 indicates homology		 X R.GVNVLADAVKVTLGPK.G
2433   807.9001 1613.7856 1613.7897 -2.49 0 67 4e-006 +1Score > 41 indicates identity
Score > 25 indicates homology		 U X R.QITANAGDEPSVVADK.V
2919   614.6603 1840.9591 1840.9530 3.27 1 39 0.00076 +1Score > 39 indicates identity
Score > 20 indicates homology		 U X R.QITANAGDEPSVVADKVK.Q
3832 +1 801.4170 2401.2292 2401.1973 13.3 1 20 0.07 +1Score > 38 indicates identity
Score > 21 indicates homology		 X K.ANDAAGDGTTTATVLAQAIVNEGLK.A + Deamidated (NQ)
3833 +2 801.4227 2401.2463 2401.2336 5.26 1 48 0.0047 +1Score > 37 indicates identity U X K.ANDAAGDGTTTATVLAQAIITEGLK.A

+31 subsets and intersections (669 subset proteins in total)

+15

Accession Score Description
1 RS2_PSEP1 297 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

+16

Accession Score Description
1 ODO2_PSEAE 292 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+17

Accession Score Description
1 SUCC_PSEPK 257 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+18

Accession Score Description
1 EFG1_PSEPK 240 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+19

Accession Score Description
1 ARPA_PSEPU 230 Antibiotic efflux pump periplasmic linker protein arpA OS=Pseudomonas putida GN=arpA PE=2 SV=1

+20

Accession Score Description
1 RL18_PSEP1 226 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
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