MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 131–140 (out of 230)


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+131

Accession Score Description
1 MREB_BACSU 46 Rod shape-determining protein mreB OS=Bacillus subtilis GN=mreB PE=3 SV=3

+132

Accession Score Description
1 RP54_RHOSH 46 RNA polymerase sigma-54 factor OS=Rhodobacter sphaeroides GN=rpoN PE=3 SV=1

+133

Accession Score Description
1 PPT2_BOVIN 46 Lysosomal thioesterase PPT2 OS=Bos taurus GN=PPT2 PE=2 SV=1

+134

Accession Score Description
1 PROB_ACIBC 46 Glutamate 5-kinase OS=Acinetobacter baumannii (strain ACICU) GN=proB PE=3 SV=1

+135

Accession Score Description
1 RS10_PSEF5 45 30S ribosomal protein S10 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsJ PE=3 SV=1

-136

Accession Score Description
1 RS8_PSEP1 45 30S ribosomal protein S8 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsH PE=3 SV=1
Score Mass Matches Sequences emPAI
136.1 RS8_PSEP1 45 14023 3 (1) 3 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsH PE=3 SV=1
13 samesets of RS8_PSEP1
RS8_PSEPG 45 14023 3 (1) 3 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas putida (strain GB-1) GN=rpsH PE=3 SV=1
RS8_PSEPK 45 14023 3 (1) 3 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas putida (strain KT2440) GN=rpsH PE=3 SV=1
RS8_PSEE4 45 14024 3 (1) 3 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas entomophila (strain L48) GN=rpsH PE=3 SV=1
RS8_PSEPW 45 14039 2 (1) 2 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas putida (strain W619) GN=rpsH PE=3 SV=1
RS8_PSEA7 45 14219 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsH PE=3 SV=2
RS8_PSEA8 45 14219 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsH PE=3 SV=1
RS8_PSEAB 45 14219 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsH PE=3 SV=1
RS8_PSEAE 45 14219 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas aeruginosa GN=rpsH PE=3 SV=1
RS8_PSEF5 45 14178 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsH PE=3 SV=1
RS8_PSEFS 45 14140 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsH PE=3 SV=1
RS8_PSEMY 45 14106 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas mendocina (strain ymp) GN=rpsH PE=3 SV=1
RS8_PSEPF 45 14166 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsH PE=3 SV=1
RS8_PSEU5 45 14047 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas stutzeri (strain A1501) GN=rpsH PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
558   372.2193 742.4240 742.4225 2.08 0 24 0.86 +1Score > 41 indicates identity
Score > 36 indicates homology
U K.AVTDLPK.V
613   386.7264 771.4382 771.4351 4.05 0 21 4 +7Score > 43 indicates identity
Score > 40 indicates homology
U R.SSRPGLR.Q
1428   566.3257 1130.6368 1130.6295 6.46 0 45 0.0014 +1Score > 39 indicates identity
Score > 29 indicates homology
U R.GGLGVSIVSTNK.G

+137

Accession Score Description
1 SYFB_METLZ 45 Phenylalanyl-tRNA synthetase beta chain OS=Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) GN=pheT PE=3 SV=1

+138

Accession Score Description
1 HPRK_AZOSE 45 HPr kinase/phosphorylase OS=Azoarcus sp. (strain EbN1) GN=hprK PE=3 SV=1

+139

Accession Score Description
1 CLPX_MICAN 45 ATP-dependent Clp protease ATP-binding subunit clpX OS=Microcystis aeruginosa (strain NIES-843) GN=clpX PE=3 SV=1

+140

Accession Score Description
1 ARLY_ACIC1 45 Argininosuccinate lyase OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) GN=argH PE=3 SV=1
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