MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 131–140 (out of 230)


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+131

Accession Score Description
1 MREB_BACSU 46 Rod shape-determining protein mreB OS=Bacillus subtilis GN=mreB PE=3 SV=3

-132

Accession Score Description
1 RP54_RHOSH 46 RNA polymerase sigma-54 factor OS=Rhodobacter sphaeroides GN=rpoN PE=3 SV=1
Score Mass Matches Sequences emPAI
132.1 RP54_RHOSH 46 48126 3 (2) 2 (1) 0.10
RNA polymerase sigma-54 factor OS=Rhodobacter sphaeroides GN=rpoN PE=3 SV=1
3 samesets of RP54_RHOSH
BIOD_XYLFA 46 24124 2 (2) 1 (1) 0.20
Dethiobiotin synthetase OS=Xylella fastidiosa GN=bioD PE=3 SV=1
BIOD_XYLFM 46 24257 2 (2) 1 (1) 0.20
Dethiobiotin synthetase OS=Xylella fastidiosa (strain M12) GN=bioD PE=3 SV=1
BIOD_XYLFT 46 24236 2 (2) 1 (1) 0.20
Dethiobiotin synthetase OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) GN=bioD PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
276   585.3362 584.3289 584.3282 1.26 0 11 2 +6Score > 37 indicates identity
Score > 27 indicates homology
R.AQLPR.I + Deamidated (NQ)
737   829.4808 828.4735 828.4705 3.68 1 45 0.03 +1Score > 42 indicates identity U R.ENLALIR.S + Deamidated (NQ)
738   415.2443 828.4740 828.4705 4.31 1 43 0.043 +1Score > 42 indicates identity U R.ENLALIR.S + Deamidated (NQ)

1 subset or intersection (7 subset proteins in total)

Score Mass Subset of
PSTB2_ERWCT 45 29327 132.1
Phosphate import ATP-binding protein pstB 2 OS=Erwinia carotovora subsp. atroseptica GN=pstB2 PE=3 SV=1
6 samesets of PSTB2_ERWCT
PSTB_BURSP 45 29824
Phosphate import ATP-binding protein pstB OS=Burkholderia sp. GN=pstB PE=3 SV=1
PSTB_RALEJ 45 29899
Phosphate import ATP-binding protein pstB OS=Ralstonia eutropha (strain JMP134) GN=pstB PE=3 SV=1
PSTB2_YERPA 45 29250
Phosphate import ATP-binding protein pstB 2 OS=Yersinia pestis bv. Antiqua (strain Antiqua) GN=pstB2 PE=3 SV=1
PSTB2_YERPE 45 29250
Phosphate import ATP-binding protein pstB 2 OS=Yersinia pestis GN=pstB2 PE=3 SV=1
PSTB2_YERPN 45 29250
Phosphate import ATP-binding protein pstB 2 OS=Yersinia pestis bv. Antiqua (strain Nepal516) GN=pstB2 PE=3 SV=1
PSTB2_YERPS 45 29250
Phosphate import ATP-binding protein pstB 2 OS=Yersinia pseudotuberculosis GN=pstB2 PE=3 SV=1

+133

Accession Score Description
1 PPT2_BOVIN 46 Lysosomal thioesterase PPT2 OS=Bos taurus GN=PPT2 PE=2 SV=1

+134

Accession Score Description
1 PROB_ACIBC 46 Glutamate 5-kinase OS=Acinetobacter baumannii (strain ACICU) GN=proB PE=3 SV=1

+135

Accession Score Description
1 RS10_PSEF5 45 30S ribosomal protein S10 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsJ PE=3 SV=1

+136

Accession Score Description
1 RS8_PSEP1 45 30S ribosomal protein S8 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsH PE=3 SV=1

+137

Accession Score Description
1 SYFB_METLZ 45 Phenylalanyl-tRNA synthetase beta chain OS=Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) GN=pheT PE=3 SV=1

+138

Accession Score Description
1 HPRK_AZOSE 45 HPr kinase/phosphorylase OS=Azoarcus sp. (strain EbN1) GN=hprK PE=3 SV=1

+139

Accession Score Description
1 CLPX_MICAN 45 ATP-dependent Clp protease ATP-binding subunit clpX OS=Microcystis aeruginosa (strain NIES-843) GN=clpX PE=3 SV=1

+140

Accession Score Description
1 ARLY_ACIC1 45 Argininosuccinate lyase OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) GN=argH PE=3 SV=1
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