MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 230)

Page: Previous 1 2 3 4 5 6 7 … 23 Next 

+11

Accession Score Description
1 ODB2_PSEPU 324 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+12

Accession Score Description
1 DHSA_ECOLI 318 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

-13

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEE4 298 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1
2 RL4_PSE14 297 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
RL4_PSEE4 298 21825 14 (9) 7 (5) 1.03
50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1
4 samesets of RL4_PSEE4
RL4_PSEP1 298 21798 14 (9) 7 (5) 1.04
50S ribosomal protein L4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplD PE=3 SV=1
RL4_PSEPG 298 21798 14 (9) 7 (5) 1.04
50S ribosomal protein L4 OS=Pseudomonas putida (strain GB-1) GN=rplD PE=3 SV=1
RL4_PSEPK 298 21798 14 (9) 7 (5) 1.04
50S ribosomal protein L4 OS=Pseudomonas putida (strain KT2440) GN=rplD PE=3 SV=1
RL4_PSEPW 298 21798 14 (9) 7 (5) 1.04
50S ribosomal protein L4 OS=Pseudomonas putida (strain W619) GN=rplD PE=3 SV=1
RL4_PSE14 297 21901 13 (9) 6 (5) 1.03
50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1
2 samesets of RL4_PSE14
RL4_PSESM 297 21928 13 (9) 6 (5) 1.03
50S ribosomal protein L4 OS=Pseudomonas syringae pv. tomato GN=rplD PE=3 SV=1
RL4_PSEU2 297 21928 13 (9) 6 (5) 1.03
50S ribosomal protein L4 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplD PE=3 SV=1

-16 peptide matches (12 non-duplicate, 4 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
339   314.6826 627.3506 627.3493 2.19 0 21 0.23 +1Score > 34 indicates identity
Score > 27 indicates homology		 X R.GPIWR.G
912   900.5561 899.5488 899.5440 5.37 1 44 0.017 +1Score > 38 indicates identity U X X R.SILAELVR  
915 +2 450.7831 899.5516 899.5440 8.51 1 52 0.002 +1Score > 37 indicates identity U X X R.SILAELVR  
984   465.3068 928.5990 928.5957 3.59 0 45 0.0023 +1Score > 31 indicates identity U X X K.VLITVSAVK.K
1043   475.2637 948.5128 948.5141 -1.33 0 23 0.095 +1Score > 41 indicates identity
Score > 26 indicates homology		 U X X R.NLPHVDVR.D
1044   317.1792 948.5158 948.5141 1.75 0 26 0.036 +1Score > 40 indicates identity
Score > 24 indicates homology		 U X X R.NLPHVDVR.D
2012 +2 707.9053 1413.7960 1413.7868 6.56 1 92 6.8e-008 +1Score > 38 indicates identity
Score > 33 indicates homology		 U X X R.LVVVQDFAVEAPK.T
2451   810.9124 1619.8102 1619.8043 3.70 1 71 1.3e-006 +1Score > 41 indicates identity
Score > 25 indicates homology		 U X R.DVQGSDPVSLIAYEK.V
2454   811.4175 1620.8204 1620.7883 19.9 1 54 8.2e-005 +1Score > 40 indicates identity
Score > 25 indicates homology		 U X R.DVQGSDPVSLIAYEK.V + Deamidated (NQ)
3117   660.9818 1979.9236 1979.9411 -8.85 1 4 2.2 +6Score > 39 indicates identity
Score > 20 indicates homology		 U X X E.FNETLVHQAVVAYMAGGR.Q + 2 Deamidated (NQ); Oxidation (M)
4042   883.4759 2647.4059 2647.3891 6.34 0 70 1.5e-005 +1Score > 35 indicates identity U X K.LTGMGLTDVLIVSDAVDQNLYLAAR.N
4046   883.8078 2648.4016 2648.3731 10.7 0 65 1.2e-005 +1Score > 35 indicates identity
Score > 28 indicates homology		 U X K.LTGMGLTDVLIVSDAVDQNLYLAAR.N + Deamidated (NQ)

4 subsets and intersections (10 subset proteins in total)

Score Mass Subset of
RL4_PSEMY 127 21543 13.1
50S ribosomal protein L4 OS=Pseudomonas mendocina (strain ymp) GN=rplD PE=3 SV=1
RL4_PSEF5 69 21936 13.1
50S ribosomal protein L4 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplD PE=3 SV=1
3 samesets of RL4_PSEF5
RL4_PSEU5 69 21685
50S ribosomal protein L4 OS=Pseudomonas stutzeri (strain A1501) GN=rplD PE=3 SV=1
RL4_PSEFS 69 21987
50S ribosomal protein L4 OS=Pseudomonas fluorescens (strain SBW25) GN=rplD PE=3 SV=1
RL4_PSEPF 69 22005
50S ribosomal protein L4 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplD PE=3 SV=1
RL4_PSEA7 58 21627 13.1
50S ribosomal protein L4 OS=Pseudomonas aeruginosa (strain PA7) GN=rplD PE=3 SV=1
3 samesets of RL4_PSEA7
RL4_PSEA8 58 21600
50S ribosomal protein L4 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplD PE=3 SV=1
RL4_PSEAB 58 21627
50S ribosomal protein L4 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplD PE=3 SV=1
RL4_PSEAE 58 21627
50S ribosomal protein L4 OS=Pseudomonas aeruginosa GN=rplD PE=3 SV=1
RL4_AZOVD 26 21688 13.1
50S ribosomal protein L4 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplD PE=3 SV=1

+14

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 297 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
2 CH601_ECOK1 101 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
3 CH60_GEOLS 90 60 kDa chaperonin OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=groL PE=3 SV=1

+15

Accession Score Description
1 RS2_PSEP1 297 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

+16

Accession Score Description
1 ODO2_PSEAE 292 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+17

Accession Score Description
1 SUCC_PSEPK 257 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+18

Accession Score Description
1 EFG1_PSEPK 240 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+19

Accession Score Description
1 ARPA_PSEPU 230 Antibiotic efflux pump periplasmic linker protein arpA OS=Pseudomonas putida GN=arpA PE=2 SV=1

+20

Accession Score Description
1 RL18_PSEP1 226 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
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