MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 121–130 (out of 230)

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+121

Accession Score Description
1 HPPD_PSEUJ 49 4-hydroxyphenylpyruvate dioxygenase OS=Pseudomonas sp. (strain P.J. 874) GN=hpd PE=1 SV=1

-122

Accession Score Description
1 RS13_PSE14 49 30S ribosomal protein S13 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsM PE=3 SV=1
Score Mass Matches Sequences emPAI
122.1 RS13_PSE14 49 13475 2 (2) 2 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsM PE=3 SV=1
10 samesets of RS13_PSE14
RS13_PSEE4 49 13362 3 (2) 3 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas entomophila (strain L48) GN=rpsM PE=3 SV=1
RS13_PSEP1 49 13362 3 (2) 3 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsM PE=3 SV=1
RS13_PSEPG 49 13362 3 (2) 3 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas putida (strain GB-1) GN=rpsM PE=3 SV=1
RS13_PSEPK 49 13362 3 (2) 3 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas putida (strain KT2440) GN=rpsM PE=3 SV=1
RS13_PSEPW 49 13348 3 (2) 3 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas putida (strain W619) GN=rpsM PE=3 SV=1
RS13_PSEF5 49 13445 2 (2) 2 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsM PE=3 SV=1
RS13_PSEFS 49 13446 2 (2) 2 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsM PE=3 SV=1
RS13_PSEPF 49 13475 2 (2) 2 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsM PE=3 SV=1
RS13_PSESM 49 13475 2 (2) 2 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas syringae pv. tomato GN=rpsM PE=3 SV=1
RS13_PSEU2 49 13475 2 (2) 2 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsM PE=3 SV=1

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
998   469.7342 937.4538 937.4505 3.55 1 37 0.044 +1Score > 41 indicates identity
Score > 36 indicates homology		 U K.FTTEGDLR.R
1235   520.7924 1039.5702 1039.5662 3.90 0 49 0.0044 +1Score > 38 indicates identity U R.IAGVNIPDNK.H

2 subsets and intersections (23 subset proteins in total)

Score Mass Subset of
RS13_PSEMY 49 13354 122.1
30S ribosomal protein S13 OS=Pseudomonas mendocina (strain ymp) GN=rpsM PE=3 SV=1
+21 samesets of RS13_PSEMY
RS13_MYCMO 37 14001 122.1
30S ribosomal protein S13 OS=Mycoplasma mobile GN=rpsM PE=3 SV=1

+123

Accession Score Description
1 GSA_ARTS2 49 Glutamate-1-semialdehyde 2,1-aminomutase OS=Arthrobacter sp. (strain FB24) GN=hemL PE=3 SV=1

+124

Accession Score Description
1 DNAB_MYCPN 48 Replicative DNA helicase OS=Mycoplasma pneumoniae GN=dnaB PE=1 SV=1

+125

Accession Score Description
1 HEMN_PSEAE 48 Oxygen-independent coproporphyrinogen-III oxidase OS=Pseudomonas aeruginosa GN=hemN PE=3 SV=2

+126

Accession Score Description
1 RS9_METCA 47 30S ribosomal protein S9 OS=Methylococcus capsulatus GN=rpsI PE=3 SV=1

+127

Accession Score Description
1 NFRA_STAAB 47 NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain bovine RF122 / ET3-1) GN=nfrA PE=3 SV=1

+128

Accession Score Description
1 IST2_YEAST 47 Increased sodium tolerance protein 2 OS=Saccharomyces cerevisiae GN=IST2 PE=1 SV=1

+129

Accession Score Description
1 CLPC_CHLTR 46 Probable ATP-dependent Clp protease ATP-binding subunit OS=Chlamydia trachomatis GN=clpC PE=3 SV=1

+130

Accession Score Description
1 Y4430_MYCUA 46 Putative S-adenosyl-L-methionine-dependent methyltransferase MUL_4430 OS=Mycobacterium ulcerans (strain Agy99) GN=MUL_4430 PE=3 SV=1
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