MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 230)


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+11

Accession Score Description
1 ODB2_PSEPU 324 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

-12

Accession Score Description
1 DHSA_ECOLI 318 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1
Score Mass Matches Sequences emPAI
12.1 DHSA_ECOLI 318 65008 9 (6) 4 (3) 0.15
Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1
3 samesets of DHSA_ECOLI
DHSA_ECOL6 318 65008 9 (6) 4 (3) 0.15
Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli O6 GN=sdhA PE=3 SV=1
DHSA_ECO57 318 65008 9 (6) 4 (3) 0.15
Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli O157:H7 GN=sdhA PE=3 SV=1
DHSA_SALTY 318 65048 9 (6) 4 (3) 0.15
Succinate dehydrogenase flavoprotein subunit OS=Salmonella typhimurium GN=sdhA PE=3 SV=1

-9 peptide matches (7 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
261   559.3227 558.3154 558.3126 5.13 0 26 0.72 +1Score > 45 indicates identity
Score > 37 indicates homology
R.DVVAR.S
1100   487.2756 972.5366 972.5352 1.44 0 38 0.059 +1Score > 42 indicates identity
Score > 39 indicates homology
U R.ATVLATGGAGR.I
1101   973.5499 972.5426 972.5352 7.58 0 50 0.008 +1Score > 42 indicates identity U R.ATVLATGGAGR.I
1749   427.5641 1279.6705 1279.6673 2.47 0 7 1.1 +2Score > 40 indicates identity
Score > 20 indicates homology
U R.IYQRPFGGQSK.N
1750   640.8430 1279.6714 1279.6673 3.23 0 57 0.00015 +1Score > 40 indicates identity
Score > 31 indicates homology
U R.IYQRPFGGQSK.N
2089   487.2839 1458.8299 1458.8195 7.13 0 74 8e-006 +1Score > 36 indicates identity U R.LGGNSLLDLVVFGR.A
2090 +2 730.4236 1458.8326 1458.8195 9.03 0 114 7.4e-010 +1Score > 36 indicates identity U R.LGGNSLLDLVVFGR.A

+13

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEE4 298 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1
2 RL4_PSE14 297 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+14

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 297 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
2 CH601_ECOK1 101 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
3 CH60_GEOLS 90 60 kDa chaperonin OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=groL PE=3 SV=1

+15

Accession Score Description
1 RS2_PSEP1 297 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

+16

Accession Score Description
1 ODO2_PSEAE 292 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+17

Accession Score Description
1 SUCC_PSEPK 257 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+18

Accession Score Description
1 EFG1_PSEPK 240 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+19

Accession Score Description
1 ARPA_PSEPU 230 Antibiotic efflux pump periplasmic linker protein arpA OS=Pseudomonas putida GN=arpA PE=2 SV=1

+20

Accession Score Description
1 RL18_PSEP1 226 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
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