MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 111–120 (out of 230)


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+111

Accession Score Description
1 TRYP_PIG 55 Trypsin OS=Sus scrofa PE=1 SV=1

+112

Accession Score Description
1 TOLQ_PSEAE 54 Protein tolQ OS=Pseudomonas aeruginosa GN=tolQ PE=3 SV=1

+113

Accession Score Description
1 RL17_CUPTR 52 50S ribosomal protein L17 OS=Cupriavidus taiwanensis (strain R1 / LMG 19424) GN=rplQ PE=3 SV=1

+114

Accession Score Description
1 PUR5_RHOFD 52 Phosphoribosylformylglycinamidine cyclo-ligase OS=Rhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118) GN=purM PE=3 SV=1

+115

Accession Score Description
1 HMW2_MYCGE 52 Cytadherence high molecular weight protein 2 OS=Mycoplasma genitalium GN=hmw2 PE=3 SV=1

+116

Accession Score Description
1 ANT3_HUMAN 52 Antithrombin-III OS=Homo sapiens GN=SERPINC1 PE=1 SV=1

+117

Accession Score Description
1 MOAC_METB6 51 Probable molybdenum cofactor biosynthesis protein C OS=Methanoregula boonei (strain 6A8) GN=moaC PE=3 SV=1

+118

Accession Score Description
1 6PGL_PSEPU 51 6-phosphogluconolactonase OS=Pseudomonas putida GN=pgl PE=3 SV=1

-119

Accession Score Description
1 RECA_PSEP1 48 Protein recA OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=recA PE=3 SV=1
Score Mass Matches Sequences emPAI
119.1 RECA_PSEP1 48 37636 2 (2) 2 (2) 0.13
Protein recA OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=recA PE=3 SV=1
2 samesets of RECA_PSEP1
RECA_PSEPG 48 37595 2 (2) 2 (2) 0.13
Protein recA OS=Pseudomonas putida (strain GB-1) GN=recA PE=3 SV=1
RECA_PSEPK 48 37636 2 (2) 2 (2) 0.13
Protein recA OS=Pseudomonas putida (strain KT2440) GN=recA PE=3 SV=1

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1376   556.8273 1111.6400 1111.6349 4.61 1 51 0.0024 +1Score > 38 indicates identity U R.ALAAALGQIER.Q
3615   750.4437 2248.3093 2248.2678 18.4 0 23 0.0081 +1Score > 28 indicates identity
Score > 15 indicates homology
U R.QGIPAISTGSLGLDIALGIGGLPK.G + Deamidated (NQ)

1 subset or intersection (15 subset proteins in total)

Score Mass Subset of
RECA_PSEFL 51 37725 119.1
Protein recA OS=Pseudomonas fluorescens GN=recA PE=3 SV=1
14 samesets of RECA_PSEFL
RECA_PSEFS 51 37613
Protein recA OS=Pseudomonas fluorescens (strain SBW25) GN=recA PE=3 SV=1
RECA_PSEPF 51 37539
Protein recA OS=Pseudomonas fluorescens (strain Pf0-1) GN=recA PE=3 SV=1
RECA_PSESM 51 37818
Protein recA OS=Pseudomonas syringae pv. tomato GN=recA PE=3 SV=1
RECA_PSETO 51 37717
Protein recA OS=Pseudomonas tolaasii GN=recA PE=3 SV=1
RECA_PSEU2 51 37892
Protein recA OS=Pseudomonas syringae pv. syringae (strain B728a) GN=recA PE=3 SV=1
RECA_PSEF5 51 37554
Protein recA OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=recA PE=3 SV=1
RECA_PSE14 51 37848
Protein recA OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=recA PE=3 SV=1
RECA_PSEAB 51 36970
Protein recA OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=recA PE=3 SV=1
RECA_PSEAE 51 36970
Protein recA OS=Pseudomonas aeruginosa GN=recA PE=3 SV=1
RECA_PSEPU 51 37623
Protein recA OS=Pseudomonas putida GN=recA PE=3 SV=1
RECA_PSEPW 51 37662
Protein recA OS=Pseudomonas putida (strain W619) GN=recA PE=3 SV=1
RECA_PSEE4 51 37664
Protein recA OS=Pseudomonas entomophila (strain L48) GN=recA PE=3 SV=1
RECA_PSEA7 51 36970
Protein recA OS=Pseudomonas aeruginosa (strain PA7) GN=recA PE=3 SV=1
RECA_PSEA8 51 36970
Protein recA OS=Pseudomonas aeruginosa (strain LESB58) GN=recA PE=3 SV=1

+120

Accession Score Description
1 PRIB_HAEIE 49 Primosomal replication protein n OS=Haemophilus influenzae (strain PittEE) GN=priB PE=3 SV=1
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