MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 101–110 (out of 230)


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+101

Accession Score Description
1 RL19_PSEE4 62 50S ribosomal protein L19 OS=Pseudomonas entomophila (strain L48) GN=rplS PE=3 SV=1

+102

Accession Score Description
1 XYLE_ECOLI 62 D-xylose-proton symporter OS=Escherichia coli (strain K12) GN=xylE PE=1 SV=1

+103

Accession Score Description
1 ADHP_ECOLI 62 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+104

Accession Score Description
1 YJJK_ECOLI 61 Uncharacterized ABC transporter ATP-binding protein yjjK OS=Escherichia coli (strain K12) GN=yjjK PE=1 SV=2

+105

Accession Score Description
1 HTPX_PSEAB 60 Probable protease htpX homolog OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=htpX PE=3 SV=1

+106

Accession Score Description
1 RL23_PSEE4 58 50S ribosomal protein L23 OS=Pseudomonas entomophila (strain L48) GN=rplW PE=3 SV=1

+107

Accession Score Description
1 CYOB_PSEPU 58 Ubiquinol oxidase subunit 1 OS=Pseudomonas putida GN=cyoB PE=3 SV=1

+108

Accession Score Description
1 PUR4_PSEPK 57 Phosphoribosylformylglycinamidine synthase OS=Pseudomonas putida (strain KT2440) GN=purL PE=3 SV=1

+109

Accession Score Description
1 PPK_PSEPK 56 Polyphosphate kinase OS=Pseudomonas putida (strain KT2440) GN=ppk PE=3 SV=1

-110

Accession Score Description
1 RL17_PSE14 56 50S ribosomal protein L17 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplQ PE=3 SV=1
Score Mass Matches Sequences emPAI
110.1 RL17_PSE14 56 14571 7 (2) 6 (2) 0.35
50S ribosomal protein L17 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplQ PE=3 SV=1
+35 samesets of RL17_PSE14

-7 peptide matches (6 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
327   311.1718 620.3290 620.3282 1.37 0 18 0.2 +1Score > 42 indicates identity
Score > 24 indicates homology
R.LAFDR.T
457   347.1890 692.3634 692.3606 4.16 0 36 0.11 +1Score > 39 indicates identity R.QGGYLR.I
694   403.7253 805.4360 805.4334 3.29 0 26 0.11 +1Score > 41 indicates identity
Score > 29 indicates homology
U K.LFNDLGK.R
1256 +1 527.8331 1053.6516 1053.6434 7.85 0 46 0.00055 +1Score > 33 indicates identity
Score > 26 indicates homology
U R.VAEPLITLAK.E
1605   605.8822 1209.7498 1209.7445 4.44 1 38 0.01 +2Score > 31 indicates identity U R.RVAEPLITLAK.E
3973   853.7509 2558.2309 2558.1959 13.7 1 16 1 +1Score > 38 indicates identity
Score > 29 indicates homology
U R.AGDNAPMAYVELVDRPVGGSVEPAE.- + Oxidation (M)

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