MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Protein families 11–20 (out of 230)

Page: Previous 1 2 3 4 5 6 7 … 23 Next 

-11

Accession Score Description
1 ODB2_PSEPU 324 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1
Score Mass Matches Sequences emPAI
11.1 ODB2_PSEPU 324 45158 11 (10) 8 (7) 0.56
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

-11 peptide matches (10 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
864   441.2886 880.5626 880.5494 15.0 0 17 1.1 +2Score > 30 indicates identity U E.QVPVIGLR.R
1066   479.2728 956.5310 956.5291 2.07 1 55 0.0021 +1Score > 41 indicates identity U R.ALDAGIELR.Y
1295 +1 536.3544 1070.6942 1070.6852 8.45 0 45 0.00038 +1Score > 23 indicates identity U K.LTLLPFLVR.A
1703   419.6107 1255.8103 1255.8016 6.89 1 31 0.0054 +1Score > 23 indicates identity
Score > 21 indicates homology		 U R.GKLTLLPFLVR.A
1704   628.9134 1255.8122 1255.8016 8.46 1 32 0.0038 +1Score > 20 indicates identity U R.GKLTLLPFLVR.A
1805   657.3604 1312.7062 1312.6987 5.76 1 44 0.012 +1Score > 40 indicates identity
Score > 38 indicates homology		 U R.TDSEQVPVIGLR.R
1845   445.9196 1334.7370 1334.7306 4.75 0 72 5e-006 +1Score > 38 indicates identity
Score > 31 indicates homology		 U R.QPGDKPLASPAVR.K
2397   802.9390 1603.8634 1603.8392 15.1 0 37 0.0018 +1Score > 38 indicates identity
Score > 22 indicates homology		 U R.VVDGMDAALFIQAVR.G
3348   1055.5131 2109.0116 2109.0015 4.84 1 104 8.7e-010 +1Score > 39 indicates identity
Score > 26 indicates homology		 U R.DFPQINATYDDEAQIITR.H
3351   704.3477 2110.0213 2109.9855 17.0 1 112 2.1e-010 +1Score > 39 indicates identity
Score > 28 indicates homology		 U R.DFPQINATYDDEAQIITR.H + Deamidated (NQ)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
ODB2_PSEAE 80 45746 11.1
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas aeruginosa GN=bkdB PE=1 SV=1

+12

Accession Score Description
1 DHSA_ECOLI 318 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+13

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEE4 298 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1
2 RL4_PSE14 297 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+14

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 297 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
2 CH601_ECOK1 101 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
3 CH60_GEOLS 90 60 kDa chaperonin OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=groL PE=3 SV=1

+15

Accession Score Description
1 RS2_PSEP1 297 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

+16

Accession Score Description
1 ODO2_PSEAE 292 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+17

Accession Score Description
1 SUCC_PSEPK 257 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+18

Accession Score Description
1 EFG1_PSEPK 240 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+19

Accession Score Description
1 ARPA_PSEPU 230 Antibiotic efflux pump periplasmic linker protein arpA OS=Pseudomonas putida GN=arpA PE=2 SV=1

+20

Accession Score Description
1 RL18_PSEP1 226 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
Page: Previous 1 2 3 4 5 6 7 … 23 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/