MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 230)

Page: 1 2 3 4 5 6 … 23 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 1648 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_LEGPA 425 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1599 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
5 EFTU_MYCS5 42 Elongation factor Tu OS=Mycoplasma synoviae (strain 53) GN=tuf PE=3 SV=1
3 EFTU_CARRP 146 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
4 EFTU_ANATD 54 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1484 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 907 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
3 ATPA_RICAH 176 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
2 ATPA_VEREI 178 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 PORF_PSESY 690 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1
2 PORF_PSEAE 143 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+5

Accession Score Description
Family member distances as a dendrogram 1 RPOB_PSEPG 513 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
5 FTSZ_PSEPK 129 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOC_PSEP1 418 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoC PE=3 SV=1
3 RPOC_PSEU5 335 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas stutzeri (strain A1501) GN=rpoC PE=3 SV=1
6 RPOC_BIFLD 91 DNA-directed RNA polymerase subunit beta' OS=Bifidobacterium longum (strain DJO10A) GN=rpoC PE=3 SV=1
4 RPOC_SYNAS 202 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1

+6

Accession Score Description
1 RS5_PSEE4 428 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

-7

Accession Score Description
1 RL3_PSEP1 378 50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1
Score Mass Matches Sequences emPAI
7.1 RL3_PSEP1 378 22703 21 (13) 9 (6) 1.65
50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1
4 samesets of RL3_PSEP1
RL3_PSEPG 378 22703 21 (13) 9 (6) 1.65
50S ribosomal protein L3 OS=Pseudomonas putida (strain GB-1) GN=rplC PE=3 SV=1
RL3_PSEPK 378 22703 21 (13) 9 (6) 1.65
50S ribosomal protein L3 OS=Pseudomonas putida (strain KT2440) GN=rplC PE=3 SV=2
RL3_PSEE4 378 22600 19 (13) 8 (6) 1.67
50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1
RL3_PSEPW 378 22663 19 (13) 8 (6) 1.67
50S ribosomal protein L3 OS=Pseudomonas putida (strain W619) GN=rplC PE=3 SV=1

-21 peptide matches (17 non-duplicate, 4 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
330   311.6823 621.3500 621.3486 2.30 0 16 1.7 +3Score > 38 indicates identity
Score > 30 indicates homology		 R.VTQFK.T
458   693.3949 692.3876 692.3857 2.74 0 61 0.0004 +1Score > 39 indicates identity U K.GFAGTIK.R
459   347.2014 692.3882 692.3857 3.64 0 22 1.6 +2Score > 39 indicates identity
Score > 36 indicates homology		 U K.GFAGTIK.R
501   715.4481 714.4408 714.4388 2.79 0 35 0.22 +1Score > 43 indicates identity
Score > 41 indicates homology		 U M.TIGVIGR.K
502   358.2283 714.4420 714.4388 4.51 0 26 1.6 +8Score > 41 indicates identity U M.TIGVIGR.K
662   793.4011 792.3938 792.3919 2.46 1 18 0.42 +2Score > 42 indicates identity
Score > 27 indicates homology		 R.GVWEFR.L
663   397.2043 792.3940 792.3919 2.74 1 12 0.95 +1Score > 42 indicates identity
Score > 24 indicates homology		 R.GVWEFR.L
1069   958.5339 957.5266 957.5244 2.37 1 54 0.0027 +1Score > 41 indicates identity U R.AVQVTVGER.R
1070   479.7712 957.5278 957.5244 3.65 1 47 0.014 +1Score > 41 indicates identity U R.AVQVTVGER.R
1351   367.5343 1099.5811 1099.5774 3.30 0 31 0.15 +1Score > 41 indicates identity
Score > 35 indicates homology		 U R.VTAAQAGHFAK.A
1352   550.7981 1099.5816 1099.5774 3.83 0 75 1.1e-005 +1Score > 41 indicates identity
Score > 38 indicates homology		 U R.VTAAQAGHFAK.A
1354   551.2931 1100.5716 1100.5614 9.27 0 54 0.00038 +1Score > 41 indicates identity
Score > 32 indicates homology		 U R.VTAAQAGHFAK.A + Deamidated (NQ)
1418 +1 565.3352 1128.6558 1128.6503 4.93 1 53 0.0017 +1Score > 38 indicates identity U R.VTVQSLEVVR.V
2506   825.4725 1648.9304 1648.9261 2.65 0 63 7.1e-005 +1Score > 34 indicates identity U K.GAVPGATGGDVVVRPAVK.A
2507 +1 550.6510 1648.9312 1648.9261 3.08 0 49 0.0018 +1Score > 34 indicates identity U K.GAVPGATGGDVVVRPAVK.A
2762 -2 882.4694 1762.9242 1762.9101 8.00 1 89 6.1e-008 +1Score > 39 indicates identity
Score > 29 indicates homology		 U K.AELFTAGQLVDVTGQSK.G
2763   882.4731 1762.9316 1762.9101 12.2 1 (80) 2.6e-007 +1Score > 39 indicates identity
Score > 27 indicates homology		 U K.AELFTAGQLVDVTGQSK.G
2764   882.4742 1762.9338 1762.9101 13.4 1 (76) 1e-006 +1Score > 39 indicates identity
Score > 28 indicates homology		 U K.AELFTAGQLVDVTGQSK.G
2766   588.9826 1763.9260 1763.8942 18.0 1 47 0.00023 +1Score > 39 indicates identity
Score > 23 indicates homology		 U K.AELFTAGQLVDVTGQSK.G + Deamidated (NQ)

7 subsets and intersections (50 subset proteins in total)

Score Mass Subset of
RL3_PSEF5 154 22646 7.1
50S ribosomal protein L3 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplC PE=3 SV=1
RL3_PSEFS 132 22664 7.1
50S ribosomal protein L3 OS=Pseudomonas fluorescens (strain SBW25) GN=rplC PE=3 SV=1
3 samesets of RL3_PSEFS
RL3_PSEPF 132 22603
50S ribosomal protein L3 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplC PE=3 SV=1
RL3_PSESM 132 22707
50S ribosomal protein L3 OS=Pseudomonas syringae pv. tomato GN=rplC PE=3 SV=1
RL3_PSEU2 132 22693
50S ribosomal protein L3 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplC PE=3 SV=1
RL3_PSE14 116 22707 7.1
50S ribosomal protein L3 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplC PE=3 SV=1
RL3_PSEMY 97 22536 7.1
50S ribosomal protein L3 OS=Pseudomonas mendocina (strain ymp) GN=rplC PE=3 SV=1
RL3_PSEU5 82 22835 7.1
50S ribosomal protein L3 OS=Pseudomonas stutzeri (strain A1501) GN=rplC PE=3 SV=1
RL3_ACISJ 61 23374 7.1
50S ribosomal protein L3 OS=Acidovorax sp. (strain JS42) GN=rplC PE=3 SV=1
+29 samesets of RL3_ACISJ
RL3_ACTP2 53 22369 7.1
50S ribosomal protein L3 OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=rplC PE=3 SV=1
11 samesets of RL3_ACTP2
RL3_ACTP7 53 22369
50S ribosomal protein L3 OS=Actinobacillus pleuropneumoniae serotype 7 (strain AP76) GN=rplC PE=3 SV=1
RL3_ACTPJ 53 22369
50S ribosomal protein L3 OS=Actinobacillus pleuropneumoniae serotype 3 (strain JL03) GN=rplC PE=3 SV=1
RL3_ACTSZ 53 22312
50S ribosomal protein L3 OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=rplC PE=3 SV=1
RL3_AGGAC 53 12929
50S ribosomal protein L3 (Fragment) OS=Aggregatibacter actinomycetemcomitans GN=rplC PE=3 SV=1
RL3_HAEDU 53 22295
50S ribosomal protein L3 OS=Haemophilus ducreyi GN=rplC PE=3 SV=1
RL3_HAEI8 53 22350
50S ribosomal protein L3 OS=Haemophilus influenzae (strain 86-028NP) GN=rplC PE=3 SV=1
RL3_HAEIG 53 22349
50S ribosomal protein L3 OS=Haemophilus influenzae (strain PittGG) GN=rplC PE=3 SV=1
RL3_HAEIN 53 22350
50S ribosomal protein L3 OS=Haemophilus influenzae GN=rplC PE=3 SV=1
RL3_HAEPS 53 22385
50S ribosomal protein L3 OS=Haemophilus parasuis serovar 5 (strain SH0165) GN=rplC PE=3 SV=1
RL3_MANSM 53 22326
50S ribosomal protein L3 OS=Mannheimia succiniciproducens (strain MBEL55E) GN=rplC PE=3 SV=1
RL3_PASMU 53 22369
50S ribosomal protein L3 OS=Pasteurella multocida GN=rplC PE=3 SV=1

+8

Accession Score Description
1 ARPC_PSEPU 369 Antibiotic efflux pump outer membrane protein arpC OS=Pseudomonas putida GN=arpC PE=2 SV=1

+9

Accession Score Description
1 RL2_PSEP1 329 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1

+10

Accession Score Description
Family member distances as a dendrogram 1 DLDH2_PSEPU 325 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4
2 DLDH_AZOVI 161 Dihydrolipoyl dehydrogenase OS=Azotobacter vinelandii PE=1 SV=1
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