MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 230)

Page: 1 2 3 4 5 6 … 23 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 1648 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_LEGPA 425 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1599 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
5 EFTU_MYCS5 42 Elongation factor Tu OS=Mycoplasma synoviae (strain 53) GN=tuf PE=3 SV=1
3 EFTU_CARRP 146 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
4 EFTU_ANATD 54 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1484 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 907 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
3 ATPA_RICAH 176 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
2 ATPA_VEREI 178 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 PORF_PSESY 690 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1
2 PORF_PSEAE 143 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+5

Accession Score Description
Family member distances as a dendrogram 1 RPOB_PSEPG 513 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
5 FTSZ_PSEPK 129 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOC_PSEP1 418 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoC PE=3 SV=1
3 RPOC_PSEU5 335 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas stutzeri (strain A1501) GN=rpoC PE=3 SV=1
6 RPOC_BIFLD 91 DNA-directed RNA polymerase subunit beta' OS=Bifidobacterium longum (strain DJO10A) GN=rpoC PE=3 SV=1
4 RPOC_SYNAS 202 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1

-6

Accession Score Description
1 RS5_PSEE4 428 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1
Score Mass Matches Sequences emPAI
6.1 RS5_PSEE4 428 17700 16 (10) 6 (4) 0.87
30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1
4 samesets of RS5_PSEE4
RS5_PSEP1 428 17712 16 (10) 6 (4) 0.86
30S ribosomal protein S5 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsE PE=3 SV=1
RS5_PSEPG 428 17712 16 (10) 6 (4) 0.86
30S ribosomal protein S5 OS=Pseudomonas putida (strain GB-1) GN=rpsE PE=3 SV=1
RS5_PSEPK 428 17712 16 (10) 6 (4) 0.86
30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1
RS5_PSEPW 428 17712 16 (10) 6 (4) 0.86
30S ribosomal protein S5 OS=Pseudomonas putida (strain W619) GN=rpsE PE=3 SV=1

-16 peptide matches (10 non-duplicate, 6 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
246   535.3007 534.2934 534.2914 3.71 0 12 0.39 +1Score > 41 indicates identity
Score > 20 indicates homology		 R.VGFGR.G
526 -1 364.7251 727.4356 727.4341 2.17 0 37 0.07 +1Score > 39 indicates identity
Score > 38 indicates homology		 K.LVQVNR.V
528   364.7255 727.4364 727.4341 3.27 0 (29) 0.15 +1Score > 39 indicates identity
Score > 33 indicates homology		 K.LVQVNR.V
527   728.4432 727.4359 727.4341 2.55 0 30 0.16 +1Score > 39 indicates identity
Score > 35 indicates homology		 K.LVQVNR.V
811   855.4952 854.4879 854.4861 2.08 1 57 0.00064 +1Score > 37 indicates identity U R.EVPAAIQK.A
812   428.2514 854.4882 854.4861 2.46 1 20 0.57 +1Score > 37 indicates identity
Score > 30 indicates homology		 U R.EVPAAIQK.A
1117   982.5229 981.5156 981.5131 2.56 0 75 1.3e-005 +1Score > 39 indicates identity U K.GTTLQYATK.A
1118   491.7656 981.5166 981.5131 3.61 0 38 0.0068 +1Score > 39 indicates identity
Score > 29 indicates homology		 U K.GTTLQYATK.A
2002   470.9525 1409.8357 1409.8242 8.14 1 3 2.3 +7Score > 33 indicates identity
Score > 19 indicates homology		 U R.AVLEVAGVQNVLAK.C
2004 +2 705.9265 1409.8384 1409.8242 10.1 1 75 1.8e-006 +1Score > 33 indicates identity
Score > 30 indicates homology		 U R.AVLEVAGVQNVLAK.C
2104 +3 734.9117 1467.8088 1467.7974 7.82 0 104 7.6e-009 +1Score > 38 indicates identity
Score > 36 indicates homology		 U R.IFTFTALTVVGDGK.G

5 subsets and intersections (124 subset proteins in total)

Score Mass Subset of
RS5_AZOVD 382 17763 6.1
30S ribosomal protein S5 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsE PE=3 SV=1
7 samesets of RS5_AZOVD
RS5_PSE14 382 17881
30S ribosomal protein S5 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsE PE=3 SV=1
RS5_PSEF5 382 17699
30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsE PE=3 SV=1
RS5_PSEFS 382 17732
30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsE PE=3 SV=1
RS5_PSEMY 382 17708
30S ribosomal protein S5 OS=Pseudomonas mendocina (strain ymp) GN=rpsE PE=3 SV=1
RS5_PSEPF 382 17769
30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsE PE=3 SV=1
RS5_PSESM 382 17778
30S ribosomal protein S5 OS=Pseudomonas syringae pv. tomato GN=rpsE PE=3 SV=1
RS5_PSEU2 382 17778
30S ribosomal protein S5 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsE PE=3 SV=1
RS5_PSEU5 285 17649 6.1
30S ribosomal protein S5 OS=Pseudomonas stutzeri (strain A1501) GN=rpsE PE=3 SV=1
RS5_ALCBS 150 17601 6.1
30S ribosomal protein S5 OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=rpsE PE=3 SV=1
4 samesets of RS5_ALCBS
RS5_PSEA7 150 17671
30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsE PE=3 SV=1
RS5_PSEA8 150 17671
30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsE PE=3 SV=1
RS5_PSEAB 150 17671
30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsE PE=3 SV=1
RS5_PSEAE 150 17671
30S ribosomal protein S5 OS=Pseudomonas aeruginosa GN=rpsE PE=3 SV=1
RS5_HAHCH 129 17639 6.1
30S ribosomal protein S5 OS=Hahella chejuensis (strain KCTC 2396) GN=rpsE PE=3 SV=1
RS5_ACTP2 57 17463 6.1
30S ribosomal protein S5 OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=rpsE PE=3 SV=1
+108 samesets of RS5_ACTP2

+7

Accession Score Description
1 RL3_PSEP1 378 50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1

+8

Accession Score Description
1 ARPC_PSEPU 369 Antibiotic efflux pump outer membrane protein arpC OS=Pseudomonas putida GN=arpC PE=2 SV=1

+9

Accession Score Description
1 RL2_PSEP1 329 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1

+10

Accession Score Description
Family member distances as a dendrogram 1 DLDH2_PSEPU 325 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4
2 DLDH_AZOVI 161 Dihydrolipoyl dehydrogenase OS=Azotobacter vinelandii PE=1 SV=1
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