MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 230)


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+1

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 1648 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_LEGPA 425 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1599 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
5 EFTU_MYCS5 42 Elongation factor Tu OS=Mycoplasma synoviae (strain 53) GN=tuf PE=3 SV=1
3 EFTU_CARRP 146 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
4 EFTU_ANATD 54 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1484 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 907 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
3 ATPA_RICAH 176 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
2 ATPA_VEREI 178 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 PORF_PSESY 690 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1
2 PORF_PSEAE 143 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+5

Accession Score Description
Family member distances as a dendrogram 1 RPOB_PSEPG 513 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
5 FTSZ_PSEPK 129 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOC_PSEP1 418 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoC PE=3 SV=1
3 RPOC_PSEU5 335 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas stutzeri (strain A1501) GN=rpoC PE=3 SV=1
6 RPOC_BIFLD 91 DNA-directed RNA polymerase subunit beta' OS=Bifidobacterium longum (strain DJO10A) GN=rpoC PE=3 SV=1
4 RPOC_SYNAS 202 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1

+6

Accession Score Description
1 RS5_PSEE4 428 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+7

Accession Score Description
1 RL3_PSEP1 378 50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1

+8

Accession Score Description
1 ARPC_PSEPU 369 Antibiotic efflux pump outer membrane protein arpC OS=Pseudomonas putida GN=arpC PE=2 SV=1

+9

Accession Score Description
1 RL2_PSEP1 329 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1

-10

Accession Score Description
Family member distances as a dendrogram 1 DLDH2_PSEPU 325 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4
2 DLDH_AZOVI 161 Dihydrolipoyl dehydrogenase OS=Azotobacter vinelandii PE=1 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
DLDH2_PSEPU 325 50093 21 (10) 14 (8) 0.56
Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4
DLDH_AZOVI 161 49707 6 (3) 4 (2) 0.14
Dihydrolipoyl dehydrogenase OS=Azotobacter vinelandii PE=1 SV=1

-22 peptide matches (21 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
212   487.3255 486.3182 486.3166 3.39 0 28 0.22 +1Score > 46 indicates identity
Score > 34 indicates homology
X K.AGIVK.N
219   501.3408 500.3335 500.3322 2.62 0 30 0.3 +1Score > 42 indicates identity
Score > 38 indicates homology
X X K.LLAGK.K
333   312.1740 622.3334 622.3326 1.32 0 20 0.46 +1Score > 41 indicates identity
Score > 30 indicates homology
X K.ITFDK.L
414   673.4001 672.3928 672.3806 18.1 0 28 0.91 +7Score > 43 indicates identity
Score > 40 indicates homology
U X K.QGLDIK.L
467   350.7220 699.4294 699.4279 2.21 0 24 0.88 +1Score > 41 indicates identity
Score > 36 indicates homology
U X K.AAQLGLK.T
468   700.4371 699.4298 699.4279 2.75 0 45 0.027 +1Score > 41 indicates identity U X K.AAQLGLK.T
524   727.4844 726.4771 726.4752 2.64 0 31 0.17 +1Score > 36 indicates identity U X X K.LIVAVGR.R
525   364.2462 726.4778 726.4752 3.64 0 25 0.17 +1Score > 36 indicates identity
Score > 30 indicates homology
U X X K.LIVAVGR.R
949   460.2493 918.4840 918.4811 3.25 0 35 0.11 +1Score > 42 indicates identity
Score > 38 indicates homology
U X K.ALLDSSWK.Y
1393   1120.6439 1119.6366 1119.6288 6.97 0 88 5.7e-007 +1Score > 38 indicates identity U X K.NLTGGVATLFK.A
1394   560.8265 1119.6384 1119.6288 8.60 0 47 0.00055 +1Score > 37 indicates identity
Score > 27 indicates homology
U X K.NLTGGVATLFK.A
1402 -1 561.3199 1120.6252 1120.6128 11.1 0 24 1.5 +2Score > 38 indicates identity
Score > 38 indicates homology
U X K.NLTGGVATLFK.A + Deamidated (NQ)
1403   561.3215 1120.6284 1120.6128 13.9 0 (23) 1.5 +2Score > 38 indicates identity
Score > 37 indicates homology
U X K.NLTGGVATLFK.A + Deamidated (NQ)
1515   390.2065 1167.5977 1167.5996 -1.69 0 18 0.16 +1Score > 40 indicates identity
Score > 22 indicates homology
U X K.ANGVTSIQGHGK.L
1516   584.8085 1167.6024 1167.5996 2.40 0 67 9.6e-006 +1Score > 40 indicates identity
Score > 29 indicates homology
U X K.ANGVTSIQGHGK.L
1549   591.2859 1180.5572 1180.5547 2.19 0 43 0.0048 +1Score > 41 indicates identity
Score > 32 indicates homology
U X R.AMAANDTGGFVK.V
2707   866.9929 1731.9712 1731.9560 8.82 0 38 0.0029 +1Score > 35 indicates identity
Score > 25 indicates homology
U X K.FDVVVIGAGPGGYVAAIK.A
2962   934.0498 1866.0850 1866.0727 6.60 1 81 4.4e-007 +1Score > 30 indicates identity U X X LGVIGAGVIGLELGSVWAR.L
2963   623.0397 1866.0973 1866.0727 13.2 1 59 5.5e-006 +1Score > 29 indicates identity
Score > 19 indicates homology
U X X LGVIGAGVIGLELGSVWAR.L
3320   697.4011 2089.1815 2089.1572 11.6 1 65 1.1e-006 +1Score > 32 indicates identity
Score > 18 indicates homology
U X M.SQKFDVIVIGAGPGGYVAAIK.S
3320   697.4011 2089.1815 2089.1572 11.6 1 63 1.6e-006 +2Score > 32 indicates identity
Score > 18 indicates homology
U X M.TQKFDVVVIGAGPGGYVAAIK.A
4039   882.4583 2644.3531 2644.3359 6.49 1 47 0.00016 +1Score > 37 indicates identity
Score > 22 indicates homology
U X K.AQMNYDLIPSVIYTHPEIAWVGK.T

2 subsets and intersections (6 subset proteins in total)

Score Mass Subset of
DLDH2_PSEAE 148 50362 10.1
Dihydrolipoamide dehydrogenase OS=Pseudomonas aeruginosa GN=lpdG PE=3 SV=1
1 sameset of DLDH2_PSEAE
DLDH_PSEFL 148 50348
Dihydrolipoyl dehydrogenase OS=Pseudomonas fluorescens GN=lpd PE=1 SV=3
DLDH2_ARATH 45 54237 10.1
Dihydrolipoyl dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana GN=LPD2 PE=1 SV=1
3 samesets of DLDH2_ARATH
DLDH_SOLTU 45 3910
Dihydrolipoyl dehydrogenase (Fragment) OS=Solanum tuberosum PE=1 SV=1
DLDH_TRYBB 45 50815
Dihydrolipoyl dehydrogenase OS=Trypanosoma brucei brucei PE=3 SV=1
DLDH2_BACSU 45 50632
Dihydrolipoyl dehydrogenase OS=Bacillus subtilis GN=bfmBC PE=3 SV=1

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